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Re: Time to shake? [Re: FarmerEric]
    #26922726 - 09/07/20 09:43 AM (3 years, 4 months ago)

Quote:

FarmerEric said:




Any wisdom for my plates? They're supposed to be wrapped in parafilm during incubation right?

And since this one is near the edge should I isolate and move to a new plate?





That is mold bro.

Yes wrap with Parafilm or Clingwrap works too.


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Re: Time to shake? [Re: Tormato]
    #26922733 - 09/07/20 09:48 AM (3 years, 4 months ago)

It’s all the same mold too which is weird.

Maybe the place you got your spores had a big outbreak of it that contaminated a lot of their stuff.  If it was your sterile technique I think there would be more satellite colonies on there.


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Edited by A.k.a (09/07/20 09:49 AM)


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Re: Time to shake? [Re: A.k.a]
    #26922846 - 09/07/20 11:19 AM (3 years, 4 months ago)

Quote:

A.k.a said:
It’s all the same mold too which is weird.

Maybe the place you got your spores had a big outbreak of it that contaminated a lot of their stuff.  If it was your sterile technique I think there would be more satellite colonies on there.




It's so damn strange bc the growth started on a spot with spores. The prints, swabs, and syringes all from different sources. WBS was cooked for 90min @15psi that should kill any spores right? I'm so confused too and a little deflated.



Do all of my plates look like mold or just the wild gt? And being wild, could that be the cause of the mold?


Edited by FarmerEric (09/07/20 11:22 AM)


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Re: Time to shake? [Re: FarmerEric]
    #26922912 - 09/07/20 12:02 PM (3 years, 4 months ago)

Also, I keep the plates and jars around 75-78 Fahrenheit  is that OK?


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Re: Time to shake? [Re: FarmerEric]
    #26922918 - 09/07/20 12:03 PM (3 years, 4 months ago)

Did you vent the pc and not start the timer until it was at 15psi?

If the spores are all from different places then the molds probably on your end. Even if it’s where the spores are it’s just mixed in and grows quicker than cube spores.


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Re: Time to shake? [Re: A.k.a]
    #26922980 - 09/07/20 12:36 PM (3 years, 4 months ago)

The green areas actually look like cube myc to me, I'd transfer them and see what happens


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Re: Time to shake? [Re: A.k.a]
    #26922981 - 09/07/20 12:36 PM (3 years, 4 months ago)

Quote:

Did you vent the pc and not start the timer until it was at 15psi?

If the spores are all from different places then the molds probably on your end. Even if it’s where the spores are it’s just mixed in and grows quicker than cube spores.






I have an AA so I purged it for the first 5 Mins of steam as directed then closed the valve, back to pressure (15psi 250 degrees) and purged again for 5 minutes as directed, closed it and brought it back to pressure then began the timer.


Edited by FarmerEric (09/07/20 12:38 PM)


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Re: Time to shake? [Re: sporecap]
    #26922985 - 09/07/20 12:37 PM (3 years, 4 months ago)

Quote:

sporecap said:
The green areas actually look like cube myc to me, I'd transfer them and see what happens





Will do as I have a few plates. BUT are there any extra precautions to take? And can I do it now?


Edited by FarmerEric (09/07/20 12:39 PM)


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Re: Time to shake? [Re: FarmerEric]
    #26922989 - 09/07/20 12:39 PM (3 years, 4 months ago)

So far it seems to me that the mold you see is coming from the syringe, since
it's also growing on your plates. You can do a control plate, just leave it empty but wrap it and store it like the others you used for your transfers. If this one stays clean, you know your technique is good.


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Re: Time to shake? [Re: sporecap]
    #26923017 - 09/07/20 12:54 PM (3 years, 4 months ago)

Quote:

sporecap said:
So far it seems to me that the mold you see is coming from the syringe, since
it's also growing on your plates. You can do a control plate, just leave it empty but wrap it and store it like the others you used for your transfers. If this one stays clean, you know your technique is good.



Plates are not from syringes. Jars are from syringe.

When you say "leave it empty" for the plate do you mean unwrap, open it in the SAB and then seal not as if I actually did inoculate?


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Re: Time to shake? [Re: FarmerEric]
    #26923268 - 09/07/20 03:16 PM (3 years, 4 months ago)

Yes, take a Petri dish with agar in the SAB, open it, do all the movements you would do when inocculating it.
But don't put any spores in. Then seal as normal and store with your other plates.
If your technique is steril, this plate will stay clean with no growth.
If it contams, you know your technique is the problem (high sporeload in the air, too much movemnts in the SAB, agar not properly sterilized, etc)


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