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Offlinesimonbelmont
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How are these looking?
    #26921682 - 09/06/20 06:10 PM (3 years, 4 months ago)

Check out these grain bags any signs of contams? Looks like yellow fluid but I think it's moisture from the grains.



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OfflineForresterM
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Re: How are these looking? [Re: simonbelmont]
    #26921738 - 09/06/20 06:37 PM (3 years, 4 months ago)

Looks like bacteria


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinesimonbelmont
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Re: How are these looking? [Re: Forrester]
    #26921773 - 09/06/20 06:57 PM (3 years, 4 months ago)

Hmmm I thought it would be fluid from grains building up or I've also read yellow is an indicator of the mycelium getting old and producing new defences against bacteria.


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OnlineA.k.aM
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Re: How are these looking? [Re: simonbelmont]
    #26921869 - 09/06/20 07:47 PM (3 years, 4 months ago)

Super bacterial. Also looks like the grain is way too wet.

How was it prepped?? Wbs has to soak a long time to get that wet.

I bet once the grain prep is better they’ll do really well next time.


Edited by A.k.a (09/06/20 07:48 PM)


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Offlinesimonbelmont
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Re: How are these looking? [Re: A.k.a]
    #26921885 - 09/06/20 07:55 PM (3 years, 4 months ago)

Ah shoot, so no way I could move these to a mono tub and get anything viable? Yeah my grain was not very well prepared I have some that are much better, but I have only spore syringes. I tried doing some agar plates but the turned out absolutely terrible even following pasty's method to a tee they are essentially plates of contamination and nothing else.


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Re: How are these looking? [Re: simonbelmont]
    #26922014 - 09/06/20 09:05 PM (3 years, 4 months ago)

When the grains are blotchy and pressed up against the walls like this, it is bacteria almost for sure. Now I had some jars that were basically fully colonized where perhaps 1 grain was blotchy like this, I spawned to bulk and they are roaring along nicely but it seems you have a much more significant bacterial problem than even my worst jar. If bacteria are in there who knows about other mold spores. Sure you can put them to bulk, transfer only the colonized grains, spawn to pasturized bulk away from your other stuff and see what happens.


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Offlinesimonbelmont
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Re: How are these looking? [Re: mescalinechemist]
    #26922044 - 09/06/20 09:24 PM (3 years, 4 months ago)

It's awful because they all have a really nice brick feel and last week when I mixed them up through the gas exchange patch I could smell pure mushroom and was so excited. Oh well I'll give it a go and if it doesn't work start again thank you all for the feedback.


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OfflineForresterM
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Re: How are these looking? [Re: A.k.a]
    #26922328 - 09/07/20 02:39 AM (3 years, 4 months ago)

Quote:

mescalinechemist said:
When the grains are blotchy and pressed up against the walls like this, it is bacteria almost for sure.




That's a good way to describe what to look for.  Especially if the liquid around the grains is cloudy or off color.

Quote:

simonbelmont said:
Hmmm I thought it would be fluid from grains building up or I've also read yellow is an indicator of the mycelium getting old and producing new defences against bacteria.




This is true.  They are producing defenses against all the bacteria that is in there :wink:



You'll have a better chance with better grain prep, but the best ides is gonna be to keep the bacteria out in the first place, not just give it a slightly shittier habitat.

The fact that the spore syringe > agar produced nothing but contamination makes me wanna suggest you maybe try a different spore syringe, and take it to agar first this time, until you get a clean culture.  Otherwise you're just wasting all this time hoping for luck.  You don't wanna do that when you can do it right :thumbup:


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OnlineA.k.aM
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Re: How are these looking? [Re: Forrester]
    #26922593 - 09/07/20 08:10 AM (3 years, 4 months ago)

Is the bag in the first pic fully colonized??

If it is then you could definitely throw some coir on top of it and fruit it that way, or even spawn a tub with 1:1 coir. My experience with bacterial spawn has been that it’ll fruit ok as long as the grain got totally colonized, no big bare spots.

If it stays partially done like your other pics then probably won’t do well. If they stall I’d mix them up and if they manage to recover a little try and top fruit them.


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LAGM2020


Edited by A.k.a (09/07/20 08:11 AM)


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