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OfflinePinkAlpha
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How long does the entire agar process take?
    #26921664 - 09/06/20 05:59 PM (3 years, 4 months ago)

I'm a little confused with how long it takes, from the TEKs I've read.

So, you inoculate the agar with spores from an inoculation loop, then wait, what, 8 weeks? Then take a slice of healthy mycelium from that dish, repeat, another 8 weeks? So a total of 16 weeks? Help me understand.


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OfflineYumyumyumyum
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Re: How long does the entire agar process take? [Re: PinkAlpha]
    #26921672 - 09/06/20 06:04 PM (3 years, 4 months ago)

Lots of conditions with that. I have not done any transfers but I have done a lot of plates both plastic and glass. Some can go pretty quickly within a few weeks others stall. I found when humidity was involved it made a mess and contaminated the plates. I am no expert but I think they 2x mass every 18-24h? I have had place I stored away for a few months that I thought we total gone but ended up growing mic. Plate is still contaminated but it has a giant monster mushroom web of a bloom that I might put to dish.


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InvisiblecoAsTal
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Re: How long does the entire agar process take? [Re: PinkAlpha]
    #26921689 - 09/06/20 06:12 PM (3 years, 4 months ago)

Quote:

PinkAlpha said:
I'm a little confused with how long it takes, from the TEKs I've read.

So, you inoculate the agar with spores from an inoculation loop, then wait, what, 8 weeks? Then take a slice of healthy mycelium from that dish, repeat, another 8 weeks? So a total of 16 weeks? Help me understand.




No way brother--

You can see mycelial growth on a plate anywhere from several days later to a couple weeks, depending on the mushroom type (and the vitality of the spores).

Then, for a transfer you take that clean uncontammed and good-growing sector slice out of the original, put it on a second plate, and often within 24-48 hours you will see the mycelium spreading onto the new plate surface.

It would only take 8 weeks to get growth if it was 45 degrees in your room...
:themoreyouknow:


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OfflinePinkAlpha
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Re: How long does the entire agar process take? [Re: coAsTal]
    #26921704 - 09/06/20 06:18 PM (3 years, 4 months ago)

I appreciate the help, but I'm still very new to this, so let me probe you for some more details.

A. How many times will I have to transfer? Only once? So spore print ---> agar, then transfer ONCE into a new plate, then drop that plate into some grain?

B. How long do I have to wait after initially innoculating agar from a spore print? Just a few days, then transfer what's there? What am I looking for to transfer?

C. How long does it take for my new plate, the one i created from the original, to be ready for agar to grain?


Edited by PinkAlpha (09/06/20 06:21 PM)


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InvisiblecoAsTal
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Re: How long does the entire agar process take? [Re: PinkAlpha]
    #26921721 - 09/06/20 06:27 PM (3 years, 4 months ago)

Quote:

PinkAlpha said:
I appreciate the help, but I'm still very new to this, so let me probe you for some more details.

A. How many times will I have to transfer? Only once? So spore print ---> agar, then transfer ONCE into a new plate, then drop that plate into some grain?




The general goal of agar is to get clean strong mycelium separated from any contamination.
IF you have clean agar, and do not introduce contamination onto the plates while you make them or nock them up, then often one transfer is enough.
You'll read people that do many, many transfers for different (good) reasons-- but the bottom line is that you can drop a clean wedge into grain as soon as you are 100% positive that it is clean and free of bacteria, mold, or other garbage that is often present on a print plate. (Prints are very rarely clean)
Quote:

PinkAlpha said:
B. How long do I have to wait after initially innoculating agar from a spore print? Just a few days, then transfer what's there? What am I looking for to transfer?




Here I will refer you back to the thousands of great threads already present in the forums-- use the resources that are here-- there are pictorials, video's, and miles of description explaining your questions. Remember-- in the end you want clean strong growing mycelium on a plate-- that's the most basic function of agar plates-- isolating the good mycelium from contaminates.

:cheers::goodluck:


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OfflineA.k.aM
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Re: How long does the entire agar process take? [Re: PinkAlpha] * 1
    #26921725 - 09/06/20 06:29 PM (3 years, 4 months ago)

lol change weeks to days maybe.

You streak spores and they usually germinate in 3-5 days. Then you can take a transfer and it usually takes 2-3 weeks for the entire plate to be covered in myc. After the first transfer you can take another one like 2 days later if you want.

For example I made this transfer on 8/31, so it’s about six days old. Plenty of growth to make another transfer but if I want to drop it into jars it’ll need a few days. They also seem to grow quicker as the culture gets bigger.



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InvisibleWall.E
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Re: How long does the entire agar process take? [Re: PinkAlpha]
    #26921729 - 09/06/20 06:32 PM (3 years, 4 months ago)

A. As many as needed. I believe after 3 transfers it's considered an isolate or culture but I think my terminology is off. Depending on what you're going for you could theoretically swab once, get great growth with no contam and wouldn't have to transfer at all. The reason you transfer is to pull good mycelium you want away from the inevitable molds and other stuff on your print.

B. It usually takes a few days from the initial inoculation to see growth. What you're on looking for on what to transfer depends on what species you're working with. Cubes have distinctive rhizomorphic strands that look like roots or tendrils, as well as tomentose growth which looks like softer fuzzy mycelium. The agar envy thread has loads of pictures. Other species have different mycelium. I have pans and pleurotus going right now and they look different from cubes. It's just something you'll get an eye for.

C. You can put it to grain when you're satisfied. Some people have really clean plates, fast growth and get it on grain as soon as possible. Some people transfer and transfer and transfer looking for the exact precise growth they want. Its all up to you as you learn and read more and look at pictures. I recommend reading a few recent pages in the agar envy thread to acquaint yourself


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