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OfflineBucknaztee
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Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
No growth in live tissue LC
    #26921495 - 09/06/20 04:14 PM (3 years, 4 months ago)

Hey folks,
    About 3 weeks ago, I tried to make LC from live tissue because I've been having too many problems with LC from spores. I used several pieces in each jar lid via biopsy. I use 1/4 pint jars with about 1 Tablespoon light karo syrup, fill the rest with clean H2o,lid with SHIP and tyvek filter, and PC for 25 minutes. I'm not seeing anything happening here gang, what went wrong? Thanks


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OfflineForresterM
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Registered: 02/05/13
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Trusted Cultivator
Re: No growth in live tissue LC [Re: Bucknaztee]
    #26921649 - 09/06/20 05:51 PM (3 years, 4 months ago)

You really should run the mycelium across a few plates of agar first to make sure it's clean before throwing it in LC.

Just one unclean tissue biopsy is enough risk but then you put several pieces more?  That's just more chances at contamination. 

I'm gonna guess it's not growing mycelium because it's ridden with bacteria but that's just a guess.

I would strongly advise you to get a clean culture on agar if you want success and stop messing with LC until you know how to make it clean :thumbup:


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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