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InvisibleThomsen
I'm a teapot


Registered: 09/27/19
Posts: 364
APE on agar
    #26921475 - 09/06/20 04:06 PM (3 years, 4 months ago)

Hey guys.

I've been cleaning up some APE from a really shitty plate


And after the third transfer the plate looks like this.


I have multiple plates that I transfered from the shitty plate I hope to clean it. But they all look the same.
It has that kinda orange look like Lion's mane has when on agar.
Is that normal for APE or is bacteria tagging along?

If any of you have some pics of APE on agar you're welcomet to post them so I can see what a culture should look like in case of the morphology being special.

I dropped the plate right before the picture. That's why it looks so messed up..


Edited by Thomsen (09/06/20 04:07 PM)


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InvisibleThomsen
I'm a teapot


Registered: 09/27/19
Posts: 364
Re: APE on agar [Re: Thomsen]
    #26922993 - 09/07/20 12:39 PM (3 years, 4 months ago)

Noone, really?


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OfflineForresterM
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Re: APE on agar [Re: Thomsen]
    #26923040 - 09/07/20 01:07 PM (3 years, 4 months ago)

I haven't grown APE but that's pretty normal for a lot of stuff. :shrug:


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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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InvisibleJHOVA
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Re: APE on agar [Re: Thomsen]
    #26923050 - 09/07/20 01:11 PM (3 years, 4 months ago)

it looks like you shot too much of your syringe on the first plate. all you need is a single drop and then let it sit without spreading across the entire surface. you can try using a sterile swab and a drop or two on it then z streaking a few plates. or you can try using josex brf pucks which makes it easy to get away from bacteria.

read this thread for how to streak agar.

how to make a diy loop
how to make sterile swabs
:peace:


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InvisibleThomsen
I'm a teapot


Registered: 09/27/19
Posts: 364
Re: APE on agar [Re: Forrester]
    #26923056 - 09/07/20 01:14 PM (3 years, 4 months ago)

Okay I was just in doubt because I think it looked so orange compared to regular cubes. :shrug:

The first plate is from a spore print, not a syringe.
And I was so tired of not getting any germination that I kinda went nuts when scraping/cutting/slicing the swab. :lol:


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OfflineForresterM
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Re: APE on agar [Re: Thomsen]
    #26923063 - 09/07/20 01:17 PM (3 years, 4 months ago)

Quote:

Thomsen said:
Okay I was just in doubt because I think it looked so orange compared to regular cubes. :shrug:




Yeah don't take my word that it's clean, I haven't grown these I just see it with a lot of other species.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Invisiblecoversall
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Re: APE on agar [Re: JHOVA] * 1
    #26923070 - 09/07/20 01:19 PM (3 years, 4 months ago)

Quote:

JHOVA said:
it looks like you shot too much of your syringe on the first plate. all you need is a single drop and then let it sit without spreading across the entire surface. you can try using a sterile swab and a drop or two on it then z streaking a few plates. or you can try using josex brf pucks which makes it easy to get away from bacteria.

read this thread for how to streak agar.

how to make a diy loop
how to make sterile swabs
:peace:




That BRF puck tek was a super interesting read! The tek I didn't know I needed to know. Thank you.


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InvisibleThomsen
I'm a teapot


Registered: 09/27/19
Posts: 364
Re: APE on agar [Re: Forrester]
    #26923074 - 09/07/20 01:21 PM (3 years, 4 months ago)

Fair enough. I'll give a couple transfers more and see how it goes. But they are less orange now that from the first and second transfer.


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OfflineA.k.aM
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Re: APE on agar [Re: coversall]
    #26923075 - 09/07/20 01:22 PM (3 years, 4 months ago)

Ape will look different for everybody, same for basically all myc.

I donโ€™t see any orange though unless you mean from the back but all my plates are like that.


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InvisibleThomsen
I'm a teapot


Registered: 09/27/19
Posts: 364
Re: APE on agar [Re: A.k.a]
    #26923530 - 09/07/20 06:16 PM (3 years, 4 months ago)

Quote:

A.k.a said:
Ape will look different for everybody, same for basically all myc.

I donโ€™t see any orange though unless you mean from the back but all my plates are like that.




Yeah from the back. My other plates doesn't do that, at least not with cube myc.
But I've made some test plates and will inoculate some bags next week.


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