Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder

Jump to first unread post Pages: 1
OfflineBucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
First time agar concerns
    #26921439 - 09/06/20 03:48 PM (3 years, 4 months ago)

Hi folks,
      I wanted to try my hand at agar since I have apparently run out of luck with LC. I ordered 10 pre-filled agar plates from Amazon and used a spore syringe purchased from a shroomery vendor. I tried to make streaks on the plate hoping I will have growth in  multiple areas.I started seeing white last night and was super stoked, but as of today they have morphed into the green bastard.
      Should I even keep them at this point? It sounds like you can have healthy myc and baddies on the same plate and transfer the goodies to a clean plate. But I aint got no goodies yet. Any advice would be appreciated, Thank You.


Extras: Filter Print Post Top
InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh 😛
I'm a teapot User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 6,067
Trusted Cultivator
Re: First time agar concerns [Re: Bucknaztee]
    #26921454 - 09/06/20 03:55 PM (3 years, 4 months ago)

If it's green that means it's sporulated in which case there is going to be spores everywhere in the plate. For being streak plates I would honestly just try again.

If it was like T4 or something and I really wanted to save the culture I would try to transfer, but for a spore master I'd try again.

I just fear that you'll be fighting contams and it'll get old after like T3 or so :shrug:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Extras: Filter Print Post Top
OfflineBucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
Re: First time agar concerns [Re: Tormato]
    #26921461 - 09/06/20 03:59 PM (3 years, 4 months ago)

Damn. Well, I have 5 plates left and 3 new syringes, so I'll try some new ones. I've obviously been reading that a spore syringe is inherently always going to have contams in it, so is getting a clean master plate just dumb luck then?


Extras: Filter Print Post Top
InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh 😛
I'm a teapot User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 6,067
Trusted Cultivator
Re: First time agar concerns [Re: Bucknaztee]
    #26921464 - 09/06/20 04:01 PM (3 years, 4 months ago)

Quote:

ZLeiterman said:
Damn. Well, I have 5 plates left and 3 new syringes, so I'll try some new ones. I've obviously been reading that a spore syringe is inherently always going to have contams in it, so is getting a clean master plate just dumb luck then?



Not necessarily but yes at the same time. Usually the contam in syringes is bacteria which I find is easier to clean up from as opposed to molds that have already begun to produce spores.


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder


Similar ThreadsPosterViewsRepliesLast post
* (Does anyone have) Step by step pics of spore print to agar and subsequent transfers maxmush 613 11 10/12/20 05:43 PM
by Churchill_85
* How to make agar 2 agar transfer? esp 6,652 7 01/08/04 11:08 AM
by YidakiMan
* transferring agar meatman 2,484 3 10/02/02 01:13 PM
by tripndicular
* agar to agar transfer ohmatic 15,620 11 01/12/21 09:00 PM
by bodhisatta
* using agar? downforpot 2,213 6 12/02/01 07:00 PM
by Anno
* Agar questions-I may have goofed blahblahblah 3,283 6 01/11/02 02:58 PM
by ar393
* Re: Agar w/ h202, any info?? 3Mshroom 2,151 8 05/08/00 04:25 AM
by mycofile
* Question about agar nd595 3,917 18 03/10/04 04:35 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
42 topic views. 21 members, 178 guests and 35 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.01 seconds on 14 queries.