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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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First time agar concerns
#26921439 - 09/06/20 03:48 PM (3 years, 4 months ago) |
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Hi folks, I wanted to try my hand at agar since I have apparently run out of luck with LC. I ordered 10 pre-filled agar plates from Amazon and used a spore syringe purchased from a shroomery vendor. I tried to make streaks on the plate hoping I will have growth in multiple areas.I started seeing white last night and was super stoked, but as of today they have morphed into the green bastard. Should I even keep them at this point? It sounds like you can have healthy myc and baddies on the same plate and transfer the goodies to a clean plate. But I aint got no goodies yet. Any advice would be appreciated, Thank You.
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: First time agar concerns [Re: Bucknaztee]
#26921454 - 09/06/20 03:55 PM (3 years, 4 months ago) |
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If it's green that means it's sporulated in which case there is going to be spores everywhere in the plate. For being streak plates I would honestly just try again.
If it was like T4 or something and I really wanted to save the culture I would try to transfer, but for a spore master I'd try again.
I just fear that you'll be fighting contams and it'll get old after like T3 or so
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Bucknaztee
Stranger

Registered: 11/21/09
Posts: 509
Last seen: 1 year, 9 months
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Re: First time agar concerns [Re: Tormato]
#26921461 - 09/06/20 03:59 PM (3 years, 4 months ago) |
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Damn. Well, I have 5 plates left and 3 new syringes, so I'll try some new ones. I've obviously been reading that a spore syringe is inherently always going to have contams in it, so is getting a clean master plate just dumb luck then?
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: First time agar concerns [Re: Bucknaztee]
#26921464 - 09/06/20 04:01 PM (3 years, 4 months ago) |
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Quote:
ZLeiterman said: Damn. Well, I have 5 plates left and 3 new syringes, so I'll try some new ones. I've obviously been reading that a spore syringe is inherently always going to have contams in it, so is getting a clean master plate just dumb luck then?
Not necessarily but yes at the same time. Usually the contam in syringes is bacteria which I find is easier to clean up from as opposed to molds that have already begun to produce spores.
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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