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OfflineCanebrake
Stranger
Registered: 05/01/20
Posts: 96
Last seen: 5 months, 12 days
Would you put this agar to grain?
    #26920559 - 09/06/20 07:02 AM (3 years, 4 months ago)

I seem to have hit or miss colonization when I put my cultures to grain. I've been transferring a lot and I'm want to check myself. To me these 2 look good to put to grain. I don't see any contams. I imagine it could look better but it looks good to me.

I'm seeing 2-3 week colonization times wether its with corn or oats. I'm also not getting full colonization. I get enough to create a small shoe box with half ass flushes. Basically I'm getting approximately 20 gram dry flushes out of 1 shoe box. Its also taking 4-6 weeks or more to get to that point.

I'm thinking of putting these two to grain.



The last ones I put to grain that looked like that was on 8-27. This is what it looks like after 10 days. Stored in a closet. Temps get about 78 degrees during the day and 72 at night when I get home. Zero light.





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OfflineEp1429
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Registered: 10/10/18
Posts: 800
Last seen: 2 years, 10 months
Re: Would you put this agar to grain? [Re: Canebrake]
    #26920566 - 09/06/20 07:05 AM (3 years, 4 months ago)

Maybe let the first plate grow out a bit more.  The clearly defined edges of the mycelium in the lower right side of plate 1 worry me a touch.  Usually when I see mycelium encircle a patch of agar like that, it’s because the agar has some bacterial contam.

Second plate looks great, though.


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OfflineCanebrake
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Registered: 05/01/20
Posts: 96
Last seen: 5 months, 12 days
Re: Would you put this agar to grain? [Re: Ep1429]
    #26920932 - 09/06/20 10:40 AM (3 years, 4 months ago)

Quote:

Ep1429 said:
Maybe let the first plate grow out a bit more.  The clearly defined edges of the mycelium in the lower right side of plate 1 worry me a touch.  Usually when I see mycelium encircle a patch of agar like that, it’s because the agar has some bacterial contam.

Second plate looks great, though.




Makes sense about the edges.


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OfflineSYF8
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Registered: 11/16/19
Posts: 189
Loc: The Mars Hotel
Last seen: 2 months, 30 days
Re: Would you put this agar to grain? [Re: Canebrake]
    #26920938 - 09/06/20 10:43 AM (3 years, 4 months ago)

Quote:

Canebrake said:
Zero light.





I would try and get a light cycle going


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OfflineCanebrake
Stranger
Registered: 05/01/20
Posts: 96
Last seen: 5 months, 12 days
Re: Would you put this agar to grain? [Re: SYF8]
    #26921854 - 09/06/20 07:41 PM (3 years, 4 months ago)

Quote:

SYF8 said:
Quote:

Canebrake said:
Zero light.





I would try and get a light cycle going



To colonize grains? Never heard of that.


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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: Would you put this agar to grain? [Re: Canebrake]
    #26922106 - 09/06/20 09:59 PM (3 years, 4 months ago)

I do grains in light or ambient light.


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