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 psilocyclops
Newb
 
Registered: 12/08/13
Posts: 607
Loc: PNW 
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 Accidental UV Mutagenesis on Psilocybe Cubensis KSSS 
#26920206 - 09/05/20 09:47 PM (3 years, 7 months ago) |
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Found a post in the r/UncleBen subbreddit where somebody grew out some rice spawn with KSSS from a multispore syringe and then spawned to bulk. The interesting part is that he used a blacklight to keep them lit while the mycelium colonized. I believe he only used the blacklight for 4 hours on the mycelium before switching to a regular light. Here is his description of what he did and a picture of the resulting fruits. I recall an experiment on here where somebody tried a few different methods to mutate cubensis fruits by utilizing UV but i don’t remember if there were any results worth writing home about. Anyway, thought you guys would appreciate the data here is what he did: “I kept my layered monotub under foil in the dark for 2 weeks, at which then I introduced FAE and Light. Just a Great Value 60 Watt LED Black Light, suspended 3.5 ft high between 2 monotubs. Had that on for 4 hours before switching to a Blue Great Value 60 Watt LED Light. Kept my light cycle at 12h On, and 12h off. With Misting and fanning for 2 min every 8 hours”
here’s the pictures of the resulting fruits. I messaged him to see if he has printed any, i want to know if these are true albinos or simply leucistic. Anybody that can link me to the old thread i’m talking about with the UV experiments i would greatly appreciate it.
 
First pic is first flush, second pic is second flush. If anybody has any information AT ALL about utilizing UV mutagenesis to produce mutated Cubensis PLEASE post it in this thread as i find this subject super interesting.
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You must have eaten, like, a hundred bucks worth of pot, and, like, 30 bucks worth of shrooms man.
 2020 mystery seed indoor LED grow https://www.growery.org/forums/showflat.php/Number/846644
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 Pliny_the_Elder
Stranger
Registered: 02/22/20
Posts: 13
Last seen: 3 years, 1 month
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 Re: Accidental UV Mutagenesis on Psilocybe Cubensis KSSS [Re: psilocyclops]
#26921064 - 09/06/20 12:13 PM (3 years, 7 months ago) |
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Mutagenesis seems unlikely, possibly just a reaction to low light. Black lights produce light in the UV-A wavelengths and I believe UV-C is required to mutate fungal cells.
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MolecularConcept
Old School Back Again
Registered: 05/24/10
Posts: 147
Loc: Denver, Co
Last seen: 11 months, 14 days
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Re: Accidental UV Mutagenesis on Psilocybe Cubensis KSSS [Re: Pliny_the_Elder]
#26921449 - 09/06/20 03:52 PM (3 years, 7 months ago) |
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UV C is what mutates and destroys cells cen get them cheap on that electronic auction site
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bodhisatta  
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Accidental UV Mutagenesis on Psilocybe Cubensis KSSS [Re: psilocyclops]
#26922538 - 09/07/20 07:25 AM (3 years, 7 months ago) |
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Shit like that happens all the time with normal lighting 
Especially in poor conditions.
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psilocyclops
Newb
Registered: 12/08/13
Posts: 607
Loc: PNW
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Re: Accidental UV Mutagenesis on Psilocybe Cubensis KSSS [Re: bodhisatta]
#26923394 - 09/07/20 04:59 PM (3 years, 7 months ago) |
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So there is probably no merit to using UV-A on mycelium for the most part? This sorta makes me want to experiment and see what kind of results i can get with the same isolate side-by-side. I will do my best to find info but for now it looks like i’ll just have to try it and see if it makes a difference.
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You must have eaten, like, a hundred bucks worth of pot, and, like, 30 bucks worth of shrooms man.
2020 mystery seed indoor LED grow https://www.growery.org/forums/showflat.php/Number/846644
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the man
still masked
Registered: 08/12/99
Posts: 6,697
Loc: C A N A D A
Last seen: 17 hours, 32 minutes
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Re: Accidental UV Mutagenesis on Psilocybe Cubensis KSSS [Re: psilocyclops]
#26930847 - 09/11/20 09:31 PM (3 years, 7 months ago) |
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those uv LED def can bleach.. but albinos happen and why we see them on the market another possibility is he got a albino var from a vendor by mistake it happens.
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Solipsis
m̶a̶d̶ disappointed scientist
Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands 
Last seen: 8 months, 3 days
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Re: Accidental UV Mutagenesis on Psilocybe Cubensis KSSS [Re: the man]
#26944241 - 09/19/20 02:00 PM (3 years, 6 months ago) |
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I think UV-A and (part of) UV-B just leads to mushrooms tanning cause like us they produce melanin (and vitamin D) to protect themselves.
The world is filled with more or less mild mutagenic factors and mutations are also an important fundamental part of the everyday going ons of genetics.
Dosing your relatively strong mutagenic factor is the whole game, to be in that ballpark range where its neither just doing effectively nothing statistically speaking, nor making your fungi killed dead or mutated in ways that makes it wish it were dead, but just good enough odds to give you something cool but viable and productive enough.
Don't know the further details of this but i can imagine a potential problem (well can also be a benefit) is depending how you go about it you might not notice various mutants if they just get outcompeted by the normies - to a certain degree you could just consider that screening so that you are not left with something annoyingly weak.
I've only seen one UV-C induced cube so far which i am trying to get back. It is one of the strains in blended MS myc LI i have (who knows exactly how many such mutants there are really in there), i gotta say it looked more Cronenberged than other mutant cubes I've seen, i think there were more things wrong with it than is typical for varieties we are used to.
UV-C mutagenesis is something I'm quite interested in and there is a thread of mine around on it, but unfortunately i have a ton of unrelated complications to deal with before i can get back to my experiments.
good luck!
By the way i'm also curious if there are possibly methods to modulate epigenetics artificially, part of it is just gene regulation by iirc exons in which mutations could possibly have far reaching consequences - not just leading to a protein being shaped a little differently but how much entire groups of genes are turned on...
am still learning myself, but perhaps methylating agents could achieve a lot more in that department vs point mutations.
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