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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
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sucking at taking clones
    #26917947 - 09/04/20 06:28 PM (3 years, 4 months ago)

heya buds, everytime i open a fruit, there's no mycelium inside it.

Its either hollow or has some kind of white fruity center.

where am i supposed to take the mycelium from?


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Willpower is the one true virtue



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OfflineMH5109
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Re: sucking at taking clones [Re: smalltalk_canceled]
    #26917975 - 09/04/20 06:42 PM (3 years, 4 months ago)

The hollow part or that white fruity center. Its all mycelium and it literally takes a cell to clone you don't need much. Iv scrapped the inside of the stipe with a needle and gotten enough tissue to put on a plate.


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Offlinethe.raven
it learns
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Re: sucking at taking clones [Re: MH5109]
    #26919164 - 09/05/20 12:12 PM (3 years, 4 months ago)

Peel open the stipe longways, scrape or cut some tissue from the interior or the stipe, transfer to a plate. It doesn't require much tissue to get growth. Tearing or peeling open the stipe can help to avoid a possible contamination vector from cutting.


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OfflineA.k.aM
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Re: sucking at taking clones [Re: the.raven]
    #26919356 - 09/05/20 01:31 PM (3 years, 4 months ago)

lol what’s a fruity center??

Lots of jokes there but no time.


But yeah just cut a piece out of the center, were you thinking there’d be like fuzzy myc inside?


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LAGM2020


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OfflineLittleBoard
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Re: sucking at taking clones [Re: A.k.a]
    #26919409 - 09/05/20 01:59 PM (3 years, 4 months ago)

I guess anything previously unexposed from the inside works. Just be clean, take a lot of samples and something will be viable for more transfers.

My first clone was simply dumped in honey LC. I had to toss 2 out of 3 jars but one was fine :laugh: With a bit of luck this  hobby can be ridiculously simple sometimes.


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Offlinesmalltalk_canceled
Babnik
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Re: sucking at taking clones [Re: LittleBoard]
    #26921791 - 09/06/20 07:11 PM (3 years, 4 months ago)

I guess I was fooled by all the hollow stems where i took small aerial myc form, and thought it "had to be like that"

I realize now that cubes can have full stems, in addition to hollow stems,
and that ive had tons of material to choose from; i just didnt understand what I was looking at

Everytime I see a new flush, the apperance of the cube can surprise me


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OfflineYumyumyumyum
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Registered: 12/21/19
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Re: sucking at taking clones [Re: smalltalk_canceled]
    #26921797 - 09/06/20 07:15 PM (3 years, 4 months ago)

I use two sets of tong tweezers to pull apart the ends without disturbing the end I am pulling a sample out of. That is what the center looks like. You take a loop and scrape the top part interior of what you separated with only making contact with a sterile loop and the interior of the stem.


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