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InvisibleLenz
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Registered: 08/17/20
Posts: 692
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Feet=dipped in agar. How do first time transfers look?
    #26916612 - 09/03/20 11:34 PM (3 years, 4 months ago)

Getting into agar, these are transfers from some of the first plates I'd inoc'd with
with a homemade Golden Teacher spore print from a third flush fruit. Excuse some of the lumpy ass ugly plates lol, didn't realize it'd cooled way too much midpour.

All are T2, 3 days in. Looking pretty decent to my eye, but hard to tell at this stage of growth. Regardless, they are all very white and fuzzy so far, don't look like cobweb or other similar looking white molds as far as I can tell which are my main worry beyond the obvious contams.

Agar recipe was 20g agar, 15g LME, 1 L water.



The pics aren't the best, I like the backlight but need to take some with overhead 45 degree light as well. Some of what appear to be odd spots in the agar are from the plastic lid underneath the plates. I'll be keeping this updated best as I can and posting to my journal occasionally.

#1


#2


#3


#4


#5


#6


#7


#8


#9



Bit early to say I think but I like 1-3, 7, and 9 look promising. The uneven half growth in 8 is interesting, probably a bad thing though right?

What do you guys think? I imagine there's a lot I'm not seeing compared to more experienced eyes.
Looking forward to answering any questions n talking about agar with yall.


--------------------
:chems:


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OfflineBiscuits
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Lenz] * 1
    #26916656 - 09/04/20 12:36 AM (3 years, 4 months ago)

They all look fine honestly, if given a bit more time... 8 is just a rhizo sector and a tomentose sector.

When I first read the title I thought you had literally dipped a foot in agar and were looking at what grew...

It looks like you've been cooling your scalpel in your receiving plate instead of the origin plate, any reason for this?


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Offlineredhandmat
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Biscuits]
    #26916713 - 09/04/20 02:23 AM (3 years, 4 months ago)

OP all your plates look good IMO. But there are some weird things, for instance number 3, what is that dot just to the right of the myc growth? Is that just a bubble, condensation or a contam colony? It could be just that many of your plates seem to have been cooled at an angle or moved while cooling. And since this is your first time I totally understand. But going forward, you want the surface to be glass clear and level so that you can spot any weird things early on.

Quote:

Biscuits said:
It looks like you've been cooling your scalpel in your receiving plate instead of the origin plate, any reason for this?




Thats definitely normal to do. Makes more sense to cool it in either a separate clean plate (for esthetics) or the receiving plate than to cool it in the origin because you may pick up shit from the plate with grows. There is nothing to pick up from a receiving plate since it has just been poured/PC'd @ 15PSI.


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OfflineBiscuits
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Re: Feet=dipped in agar. How do first time transfers look? [Re: redhandmat]
    #26920311 - 09/05/20 11:31 PM (3 years, 4 months ago)

I just figured it was one more time you had to open up the receiving plate, one more time you had to hover your hand over it... So more chances for something to drop into a completely clean plate.

I've always cleaned mine on the origin... The red hot scalpel will kill anything it touches on the origin plate (I've never had a plate without a clean area to cool in anyway), so I'm not worried about taking something over like that... but I am worried about opening the receiving plate more than I need to.


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Offlineverum subsequentis
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Biscuits]
    #26920323 - 09/05/20 11:51 PM (3 years, 4 months ago)

Once good with sterile tek, in a flow or sab, there need be no fear of opening a plate to cool the scalpel. Done correctly nothing will get in. It is absolutely recommended to cool in the receiving plate for the reasons stated above. While you may think the red hot scalpel will kill anything it touches, this is not true. I could explain why but don't feel like typing that much.

OP, your plates are looking great. I'd advice taking a transfer from the fastest growing section of each to new plates (poured a tad warmer this time) and then updating with pics about a week later. I'm fairly certain you'd do fine putting those to grain as is but another transfer would be a better idea.

The pic with the really obviously different sides is quite normal. It's not just "a rhizo sector and a tom sector". It's thousands and thousands of genetics doing a little dance and trying to figure out who's who in the zoo. While it's possible that the presence of some nasty is causing the one side to fluff up I don't think it's necessarily so. Any who, definitely take from the nice side of that one and not the fugly side.

Good work sailor. I love seeing a noob dip the proverbial foot in the boiling agar pool. Makes me proud


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OfflineBiscuits
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Re: Feet=dipped in agar. How do first time transfers look? [Re: verum subsequentis]
    #26920398 - 09/06/20 02:17 AM (3 years, 4 months ago)

Well I learnt something new today, thank you both :thumbup:


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Offlinethe.raven
it learns
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Biscuits]
    #26920760 - 09/06/20 09:15 AM (3 years, 4 months ago)

The plates look good. More importantly, you are observing how differences in the process effect end results. Good job. An IR thermometer is great for no-contact temperature measurements of your agar.

I ran an experiment with my agar bottles after a PC cycle. I recorded ambient temp, time, and took temp measurements of my agar bottles every 5 minutes. I loaded the data set into a spreadsheet and graphed it. It has been a helpful tool to assess how long I have to wait for my agar to be at pour temp. Now I take an initial read on my hot agar, check the graph, set an alarm, and go work on other things while I wait for pour temp. Perhaps you'll find it valuable.


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Offlinetryptkaloids
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Re: Feet=dipped in agar. How do first time transfers look? [Re: the.raven]
    #26920820 - 09/06/20 09:42 AM (3 years, 4 months ago)

You arent supposed to dip your feet in it :horrified:
:trololol:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Edited by tryptkaloids (09/06/20 09:42 AM)


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Offlinejcm4620
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Re: Feet=dipped in agar. How do first time transfers look? [Re: tryptkaloids]
    #26920935 - 09/06/20 10:43 AM (3 years, 4 months ago)

Quote:

tryptkaloids said:
You arent supposed to dip your feet in it :horrified:
:trololol:



you fucker

u beat me to it😂😂😂😂


--------------------
PANAEOLUS FRUITING MADE SIMPLE



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Offlinetryptkaloids
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Re: Feet=dipped in agar. How do first time transfers look? [Re: jcm4620]
    #26921073 - 09/06/20 12:16 PM (3 years, 4 months ago)

You fucker.
You brought her
You fuck her, you killed her


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleGoatrider
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Registered: 04/08/20
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Re: Feet=dipped in agar. How do first time transfers look? [Re: redhandmat]
    #26921111 - 09/06/20 12:39 PM (3 years, 4 months ago)

Quote:

redhandmat said:
OP all your plates look good IMO. But there are some weird things, for instance number 3, what is that dot just to the right of the myc growth?





You see marks in the plastic of the dishes :grin:


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OfflineWunFunGai
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Registered: 05/03/16
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Lenz]
    #26921212 - 09/06/20 01:34 PM (3 years, 4 months ago)

OP looking good! :thumbup:

I've recently started my first time with plates and have been logging it in another thread with my first attempts at isolating from spores from a fruit that came from a good performing clone from long ago.

I've only done a few PastyPlates w/ Glad cups prior to this and I was nervous about agar in a dish (shouldn't have) and I'm pretty sure I had some fugly looking agar the first couple times, but the frosty glad cups hid any cosmetic flaws. I wanted to see clearly and plates have literally been an eye opener...not sure why I was so hesitant and waited so long.

Look forward to seeing your progress!

WFG :smile:


--------------------
The Official TNF Reccomended Teks & Methods
Bod's Simplified Cultivation Methods <-- Awesome!!!
D3monics Perfect Transfers and Agar Tek

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You gotta be here, if you're not all there...


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Offlineverum subsequentis
seeker of truth
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Re: Feet=dipped in agar. How do first time transfers look? [Re: WunFunGai]
    #26921251 - 09/06/20 01:57 PM (3 years, 4 months ago)

i prefer petris for that exact reason. they're only intimidating at first but really really easy once you get the hang of it.


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Offlinejcm4620
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Re: Feet=dipped in agar. How do first time transfers look? [Re: verum subsequentis]
    #26921253 - 09/06/20 02:00 PM (3 years, 4 months ago)

i want glass pitris so bad but for as many as id like that shits fucking expensive


--------------------
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Offlineverum subsequentis
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Re: Feet=dipped in agar. How do first time transfers look? [Re: jcm4620]
    #26921377 - 09/06/20 03:13 PM (3 years, 4 months ago)

i feel ya


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Offlineverum subsequentis
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Re: Feet=dipped in agar. How do first time transfers look? [Re: verum subsequentis]
    #26921381 - 09/06/20 03:14 PM (3 years, 4 months ago)

It's honestly cheaper in the long run though. I did the math a while ago and can't remember exactly what i found but it was something around three years and they'd have paid for themselves.


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Offlineredhandmat
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Registered: 05/09/19
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Goatrider]
    #26922373 - 09/07/20 04:04 AM (3 years, 4 months ago)

Quote:

Goatrider said:
Quote:

redhandmat said:
OP all your plates look good IMO. But there are some weird things, for instance number 3, what is that dot just to the right of the myc growth?





You see marks in the plastic of the dishes :grin:




Hahahhaha! I just saw it. I usually work in glass petri so sometimes I forget to look for these things in peoples pictures.


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InvisibleLenz
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Registered: 08/17/20
Posts: 692
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Re: Feet=dipped in agar. How do first time transfers look? [Re: redhandmat]
    #26925539 - 09/09/20 12:10 AM (3 years, 4 months ago)

Hey ya'll I've had a busy couple of days but thanks for all the great responses! Appreciate it greatly :grin:

I had a bit of a stupid mishap with my agar prep recently and burned my torso lol. I popped the weight off my PC when it hit zero to tighten the lid on my 1L media bottle as per c10's agar guide but after doing so I fucking didn't think at all and just put it in a pot of room temp water to cool off which caused the bottle to explode. Luckily the glass didn't go far and the agar must've cooled quite a bit before hitting me lol but I have some gnarly burns now. That prep overall was a big disaster I was distracted the whole time and trying to do multiple things at once. Lesson learned.

Unfortunately, in the couple of days that have passed, some kind of fuzzy offwhite/orangeish cobweb like mold has appeared to have taken over almost all of the plates. I definitely assumed that all of these plates were going to be a-okay but I'm glad I waited a bit for further grow. Here are some pics:

#1


#2


#3


#4



#5



#6



#7


#8




Even the plates that are looking okay here, #1,3, and 4, all appear to have a very subtle thin hairy layer of growth on top of the myc. Very disappointing. I would try cleaning it up by transferring but I don't want to waste my time if it is indeed something like cobweb that has grown everywhere. You can see it much more clearly on 6-8, it's like an almost orange/grey fuzzy growth.

I'm not sure where exactly I went wrong. Everything was originally transferred from glad mini rounds inoculated with a sloppily made spore print but it's frustrating because I can't tell at which point things went bad. I suspect it's most likely the initial inoculation from the spore print and that I couldn't really distinguish the cobweb at all from the other myc growth because I did a very poor job of diluting the spores and streaking them correctly and because of the visibility of the minirounds. If that was the case I easily could have transferred a bunch of contam'd pieces. Otherwise I guess it could have been my sterile technique during the transfers but the fact that essentially all of plates show this same kind of contam makes me think that's not so likely.


Anyone have any tips?? Thoughts on the plates? I'm going to try transferring from some of the better looking plates like #4 but I don't have high hopes. I'll be posting some more pics later from a second batch of transfers that I did the day after these and those are looking even worse. Learning experience I guess, just sucks that I had to be hit with a contam like this so early on.


--------------------
:chems:


Edited by Lenz (09/09/20 12:23 AM)


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InvisibleLenz
Misunderestimated
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Registered: 08/17/20
Posts: 692
Loc: Fungistan
Re: Feet=dipped in agar. How do first time transfers look? [Re: WunFunGai]
    #26925546 - 09/09/20 12:18 AM (3 years, 4 months ago)

Quote:

verum subsequentis said:
Once good with sterile tek, in a flow or sab, there need be no fear of opening a plate to cool the scalpel. Done correctly nothing will get in. It is absolutely recommended to cool in the receiving plate for the reasons stated above. While you may think the red hot scalpel will kill anything it touches, this is not true. I could explain why but don't feel like typing that much.

OP, your plates are looking great. I'd advice taking a transfer from the fastest growing section of each to new plates (poured a tad warmer this time) and then updating with pics about a week later. I'm fairly certain you'd do fine putting those to grain as is but another transfer would be a better idea.

The pic with the really obviously different sides is quite normal. It's not just "a rhizo sector and a tom sector". It's thousands and thousands of genetics doing a little dance and trying to figure out who's who in the zoo. While it's possible that the presence of some nasty is causing the one side to fluff up I don't think it's necessarily so. Any who, definitely take from the nice side of that one and not the fugly side.

Good work sailor. I love seeing a noob dip the proverbial foot in the boiling agar pool. Makes me proud





Thank you for the kind words! Appreciate your presence here and elsewhere on the boards, I just posted an update and I think I messed up by not transferring these earlier. Oh well.


Quote:

the.raven said:
The plates look good. More importantly, you are observing how differences in the process effect end results. Good job. An IR thermometer is great for no-contact temperature measurements of your agar.

I ran an experiment with my agar bottles after a PC cycle. I recorded ambient temp, time, and took temp measurements of my agar bottles every 5 minutes. I loaded the data set into a spreadsheet and graphed it. It has been a helpful tool to assess how long I have to wait for my agar to be at pour temp. Now I take an initial read on my hot agar, check the graph, set an alarm, and go work on other things while I wait for pour temp. Perhaps you'll find it valuable.




Thanks! I am trying to keep the process and otherwise important variables in mind as I work but I may need to start taking more notes or something so I can more easily track contam's or such when they appear.

And the tip about the IR thermometer is a great one! I'm too broke to buy one now but I'll keep it in mind.


Quote:

WunFunGai said:
OP looking good! :thumbup:

I've recently started my first time with plates and have been logging it in another thread with my first attempts at isolating from spores from a fruit that came from a good performing clone from long ago.

I've only done a few PastyPlates w/ Glad cups prior to this and I was nervous about agar in a dish (shouldn't have) and I'm pretty sure I had some fugly looking agar the first couple times, but the frosty glad cups hid any cosmetic flaws. I wanted to see clearly and plates have literally been an eye opener...not sure why I was so hesitant and waited so long.

Look forward to seeing your progress!

WFG :smile:




Hey, thanks! I've actually seen your thread before, was a bit of an inspiration, all your pics and plates looked so good, was very cool. Keep it up!

I started with pastyplates too exactly because I was scared about pouring the agar without getting contam's but after making like 30 pastyplates I realized I can't keeping doing this lol. The lack of visibility was really bugging me as well, I wanted to train my eye and that was just way harder with pasty's. Glad I made the switch.


--------------------
:chems:


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Offlineverum subsequentis
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Registered: 03/22/16
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Re: Feet=dipped in agar. How do first time transfers look? [Re: Lenz]
    #26925591 - 09/09/20 01:20 AM (3 years, 4 months ago)

Disclaimer, I'm colorblind and have been dabbling in some wonderful whiskey BUT I'm not sure there's anything wrong with those plates. I'll check again tomorrow to find out.

What colors did you use to make up the agar? If you used any red at all I might know what's happening.


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