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OfflineAckso
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Registered: 08/15/20
Posts: 36
Last seen: 2 years, 11 months
Reinoculation
    #26916267 - 09/03/20 07:37 PM (3 years, 4 months ago)

Hey guys! So I inocculated 18 bags with with spore syringes of 2 sources. A controlled test if you will. 9 bags from spores101 ( yes I see a lot of people on here dont recomend them, but i know that now!) and 9 bags from a secondary vender. Anyways long story short.. 20 days after inocculation spores101 has no activity (2 moldy bags and 7 not active) while all 9 bags from the secondary vender look fantastic. My question is this; can i reinoculate the 7 bags because i know it was the spores and not my tech? Or is it too risky at this point for contams? Thanks for any insights!


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OfflineSpore211215
SporeGuy
Male

Registered: 06/06/16
Posts: 108
Last seen: 1 year, 8 months
Re: Reinoculation [Re: Ackso]
    #26916301 - 09/03/20 07:54 PM (3 years, 4 months ago)

I say you can try if you want but your risk of getting contammed out at this point is well into the 90's. If you have a lot of MSS burning a hole in your pocket then sure, otherwise don't waste your time.

*Edit* Spelling error


Edited by Spore211215 (09/03/20 08:31 PM)


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Offlinelookintolearn
Stranger becoming Strangest
I'm a teapot User Gallery


Registered: 06/02/20
Posts: 574
Loc: Up in the Cut
Last seen: 1 month, 18 days
Re: Reinoculation [Re: Spore211215]
    #26916305 - 09/03/20 08:03 PM (3 years, 4 months ago)

:whathesaid:

I agree. Chances are slim to none on a re-inoculation especially with MS syringe.


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Offline1kldude
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I'm a teapot


Registered: 05/15/08
Posts: 501
Loc: Somewhere
Last seen: 2 years, 10 months
Re: Reinoculation [Re: lookintolearn]
    #26916374 - 09/03/20 08:42 PM (3 years, 4 months ago)

Ive inoculated 1 BRF jar as a test. I poked it through the micropore tape and promptly put more tape over the hole and it worked without contamination. Now that is just 1 time so i don't know if that would work every time.


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OfflineAckso
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Registered: 08/15/20
Posts: 36
Last seen: 2 years, 11 months
Re: Reinoculation [Re: 1kldude]
    #26917743 - 09/04/20 04:15 PM (3 years, 4 months ago)

Thanks for the input guys! Heres another thought, i could just re steralize the bags right?


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Offline1kldude
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I'm a teapot


Registered: 05/15/08
Posts: 501
Loc: Somewhere
Last seen: 2 years, 10 months
Re: Reinoculation [Re: Ackso]
    #26917787 - 09/04/20 04:42 PM (3 years, 4 months ago)

Quote:

Ackso said:
Thanks for the input guys! Heres another thought, i could just re steralize the bags right?



You would be killing the mycellium.
Are you doing that because of mold? Wouldnt want dead mold in my substrate.
But in theory i think that would work. But why not just use new substrate?


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<<-----|||1kldude|||----->>

<<<----------|||1 knowledgeable dude|||---------->>>

<<<<----Any post I make is purely fictional and for informational purposes only---->>>>


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OfflineGan
Wielder of Narya
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Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 5 months, 27 days
Re: Reinoculation [Re: 1kldude]
    #26917852 - 09/04/20 05:25 PM (3 years, 4 months ago)

You could technically re-sterilize them. However, that will dry them out more than likely.


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OfflineAckso
Stranger
Registered: 08/15/20
Posts: 36
Last seen: 2 years, 11 months
Re: Reinoculation [Re: 1kldude]
    #26917855 - 09/04/20 05:27 PM (3 years, 4 months ago)

Im talking about re sterilizing the 7 bags that have no mold , no activity at all..


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