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OfflineYumyumyumyum
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Registered: 12/21/19
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Last seen: 4 months, 30 days
Some input on a FC design and a question about agar transfers.
    #26916123 - 09/03/20 06:04 PM (3 years, 4 months ago)

I had to reset everything last month just to rid myself of multiple problems. Now everything is back in great shape. One of the issues I had was opening and closing my FC. I got bugs and they caused all kinds of problems. Now I have tried to keep it enclosed. I took a humidifier and attached the other end via hose to the top of my FC with a gasket. Its running high 90's humidity and a low 70's temp. Can anyone think of any issues with this or adjustments? I use a USB fan to give it FAE 4+ times a day. Light cycle is 12h a day cakes looking good(from one of the samples below, a real work horse).




Second these were some dishes I had stored away in a box because they look toasted. They are a few months old and I noticed one even had aborts. Is it possible to pull a sample and transfer it to another plate to cultivate another cleaner sample which in turn can itself be transferred? I know most of these plates are busted 2 have been used over a 9 month period with good results. They have all been replaced by new cultures.

http://www.shroomery.org/forums/thumbs/20-36/917722584-thumb_P9020002.jpg[/image][/url] [url=https://files.shroomery.org/files/20-36/917723372-P9020007.jpg][/url


Edited by Yumyumyumyum (09/03/20 06:06 PM)


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Invisiblefilthyknees
no coincidence
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Registered: 03/08/13
Posts: 6,283
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Re: Some input on a FC design and a question about agar transfers. [Re: Yumyumyumyum]
    #26916829 - 09/04/20 06:08 AM (3 years, 4 months ago)

Why are you trying to make your own fruiting chamber when there are perfectly good teks that are tried and true like the monotub Tek or pastys ez dialed tubs. I'd scrap that to be honest.

The plate looks questionable. Take a transfer if you want but start fresh. I can send you fresh clean spores if you want, wouldn't place bets on that agar.

The environment needs way less controls that you think, these fc teks are mostly set and forget passive, no automation fans and pumped in humidifiers.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflineYumyumyumyum
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Registered: 12/21/19
Posts: 412
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Last seen: 4 months, 30 days
Re: Some input on a FC design and a question about agar transfers. [Re: filthyknees]
    #26919105 - 09/05/20 11:33 AM (3 years, 4 months ago)

Thank you for the input

That is my old fruiting chamber was trying something new with 2 cakes I had. I have a new box for fruiting but one of the issues I had this summer was A. Nats B. Cakes stalling and getting contaminated. C. going from 4-6 flushes to 1) Part of the problem is my FC was too close to my indoor plants. No they have a nice space running air filtration. What I was trying to do it have the humidity high and FAE out not in. I am just trying to limit the amount of times I need to open the case. The cakes are in great shape. I have a week or two before my project birthing is back on schedule after a disastrous July August. That chamber has evolved over 11 months(Bad,good,great,amazing,bad) you cannot regress the the modifications so I am trying one last thing before I toss and use a new one. 

The agar plates were ones I tossed in a box as toast and decided to check them to see what interesting things grew. Low and behold there was so really strong mic growth. Having failed at agar more than succeeded I was missing the step of procreating a good sample. I was basically one going on step on agar and not transferring. I have a ton of plates both glass and plastic I just did not feel the return and effort was worth more than some cool samples for my triscope.

I agree simple worked well but under certain conditions. That is what changes, ambient heat from summer, more particulates in the air etc etc.


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