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Offlinemanic231
Stranger
Registered: 05/11/04
Posts: 13
Last seen: 19 years, 1 month
Karo Tek Concerns
    #2691119 - 05/17/04 12:17 PM (19 years, 10 months ago)

I have been reading a lot latly about the Karo Tek, and it seems like quite frankly the greatest thing to come to growers since the PF TEK. I have however one concern. Many growers have stated that although growing with a clone is faster than with spores, repeated cloning eventually leads to the deteriorization of the strain. Basically, the question is, since you can innoculate another Karo jar with the mycilia from an already colonized Karo jar, does that lead to the deterioration of the strain like cloning, or can one spore syringe theoretically produce an unlimited amount of mycelia using the Karo Tek repeatedly. Any comments are appreciated as always.

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OfflineJiggly
still learnin'
Registered: 05/15/04
Posts: 92
Loc: CT, USA
Last seen: 19 years, 9 months
Re: Karo Tek Concerns [Re: manic231]
    #2691188 - 05/17/04 12:41 PM (19 years, 10 months ago)

im not sure because im a noob... pathetic, i know, but just make a spore syringe from the shrooms that grow, and use those spores to make more karo. if ur not sure how to make a spore syringe

Edited by Jiggly (05/17/04 12:44 PM)

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OfflineIfIWereARichMan
journeyman
Registered: 10/27/03
Posts: 84
Last seen: 12 years, 8 months
Re: Karo Tek Concerns [Re: Jiggly]
    #2691567 - 05/17/04 02:02 PM (19 years, 10 months ago)

I think Stamets wrote something about senescence occurring after 3 generations. So you innoculate a single karo jar with spores. The mycelium is then used to innoculate a dozen cakes, each of which are first generations. Then, you use a bit of mycelium from one cake to knock up a dozen more cakes. Generation 2. If you were to repeat the process, you'd be at the 3rd.

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Offlinemanic231
Stranger
Registered: 05/11/04
Posts: 13
Last seen: 19 years, 1 month
Re: Karo Tek Concerns [Re: IfIWereARichMan]
    #2691622 - 05/17/04 02:15 PM (19 years, 10 months ago)

What I mean is, can you use the mycelia from one Karo to innoculate another without having to worry about the genetic break down?

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Offlinehomelessheathen
newbie
Male
Registered: 01/29/04
Posts: 33
Loc: Florida
Last seen: 4 years, 8 months
Re: Karo Tek Concerns [Re: manic231]
    #2693279 - 05/17/04 08:36 PM (19 years, 10 months ago)

I think to reset the genetic clock, you have to start over from a spore print. it could be an old one, or a new one from a recent batch. or even the same original spore syringe if it's been stored well and hasn't gotten stale.

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OfflineHrethic
A Human, Being

Registered: 01/05/04
Posts: 2,397
Loc: Passing through the bulk Flag
Last seen: 3 years, 10 hours
Re: Karo Tek Concerns [Re: manic231]
    #2693332 - 05/17/04 08:45 PM (19 years, 10 months ago)

Run a search for it. I know i've read something like that here recently. :mushroom2:

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OfflineTodcasil
rogue DMT elf
Female User Gallery

Registered: 08/08/99
Posts: 16,381
Loc: Crawling on the floor...
Last seen: 9 years, 6 months
Re: Karo Tek Concerns [Re: manic231]
    #2693595 - 05/17/04 09:42 PM (19 years, 10 months ago)

i see what you are saying... you make a batch of karo mycelia...  you innoc a few jars but have leftover mycelia.

your qeustion: can i keep using the mycelia?

my answer: yes you can :thumbup:

or perhaps your question is can you make another batch of karo with some mycelia from the first? yes you can!

but id stick with the three generation rule of thumb that was stated by IfIWasARichMan from stamets. (as far as three tubs of karo mycelia which is a lot of syringes worth).

if those werent your Qs im sorry i misunderstood!

also IMO i dont think the genetic breakdown would happen as fast from the method you state, so just try it until something wierd happens... genetic breakdown isnth the end of the world, just a botched grow, but you will learn through the great teacher of experience : )


--------------------
Men look at themselves and they see flawed humans, we look at women and we see perfect
GODDESSES
Women look at themselves and they seem utterly human, when looking at men they see proud
GODS.


~Casil



:cactus:

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Offlinemanic231
Stranger
Registered: 05/11/04
Posts: 13
Last seen: 19 years, 1 month
Re: Karo Tek Concerns [Re: Todcasil]
    #2695411 - 05/18/04 09:44 AM (19 years, 10 months ago)

Thank you guys so much, and yea that was pretty much my question.

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