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Offlinebighead8181
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Registered: 10/09/19
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Failed Mycelium Growth
    #26915602 - 09/03/20 12:36 PM (3 years, 4 months ago)

I inoculated eight jars.  6 have no growth at all and 2 have mycelium growth on one side only.  The other 3 holes failed. 

I believe i heated the syringe needle and didn't let it cool sufficiently before adding the spores.  Question is can i reuse the failed jars?  I can sterilise the substrate in the jars again in a pressure cooker and inject spore again.  It been 15 days since inoculation.


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OfflineA.k.aM
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Registered: 10/27/19
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Re: Failed Mycelium Growth [Re: bighead8181]
    #26915642 - 09/03/20 12:51 PM (3 years, 4 months ago)

It’s probably not from the hot needle, they’re so thin they cool off almost instantly once water touches it and there’s a ton of spores moving through it.

The other jars have nothing growing no contams or anything? If that’s the case that’s pretty odd. I would say maybe there’s not many spores in the syringe but it would have to be crazy diluted for that.


You could try reusing them but I would be worried about moisture content from sitting for weeks and being cooked again.


--------------------
LAGM2020


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