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Offlinepitriot
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Agar vs Grain?
    #26915183 - 09/03/20 08:38 AM (3 years, 4 months ago)

How much longer does it take for grain to colonize when doing agar to grain vs grain to grain?


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OfflineGan
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Re: Agar vs Grain? [Re: pitriot]
    #26915223 - 09/03/20 09:12 AM (3 years, 4 months ago)

G2g will almost always be a good bit faster than a2g.


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OfflineBrian Jones
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Re: Agar vs Grain? [Re: pitriot]
    #26915230 - 09/03/20 09:18 AM (3 years, 4 months ago)

It takes significantly longer. I let agar to grain sit for a week before shaking, and then you still have a small level of contact points compared to grain to grain, which is an added delay. You might have to shake twice.

But you're trading that extra time for a confidence level that you didn't inoculate your jars with a contaminant. When doing G2G where did the donor jar come from? If made from a spore syringe you have low confidence. If made previously from agar, higher confidence but not as high as going from a petri to jars IMO.


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