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Offlinesmalldaddy
Mushroom Enthusiast
Registered: 12/03/19
Posts: 5
Loc: United States
Last seen: 3 years, 7 hours
Trich contamination issues, HELP PLS
    #26911647 - 09/01/20 10:51 AM (3 years, 5 months ago)

Hi all! So I am a biochemist by training, with 3 years experience in a microbiology lab. Basically, I consider myself versed in sterile technique. I recently moved to a new, bigger house in hopes to scale up my grow. However, I have now been here for four months with not one successful monotub. I grew at my old, crusty apartment just fine for about a year. All of the tubs at my new place get grossly contaminated with trich. I recently made a semi-sterile room with panda film, a 600sq ft HEPA air filter is in there now as we realized the house is basically infested with mold spores. The room is very small maybe like 30 sq ft, so the filter should get anything that comes in there. However, this morning, the first tub I made with the HEPA filter in there is growing trich. I feel very helpless at this point. I inspect all my jars, they all look very clean and healthy, PC for 2 hours minimum in quart jars. I bleach everything I come into contact with to spawn into the tub. I am now doing everything in the room to try to maintain sterility. Does anyone have any recommendations? I've invested so much time and effort into this at this point, it is starting to hurt my soul.I use Bod's monotub tek if you are wondering.Thank you in advance!


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OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 13 days, 2 hours
Re: Trich contamination issues, HELP PLS [Re: smalldaddy]
    #26911661 - 09/01/20 10:58 AM (3 years, 5 months ago)

Lets see some agar pics.

Lets see some tub pics.

Pretty much the only way is that your spawn wasn't as clean as you thought, now we have to figure out why?


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Offlinesmalldaddy
Mushroom Enthusiast
Registered: 12/03/19
Posts: 5
Loc: United States
Last seen: 3 years, 7 hours
Re: Trich contamination issues, HELP PLS [Re: The Mycologist]
    #26911666 - 09/01/20 11:02 AM (3 years, 5 months ago)

So I don't use agar yet, I have been doing g2g transfers for a while with great success. I recently ordered spores and they were contaminated/ did not germinate. I always order from the same website, but this batch I got was bunk. I barely ever get trich in my jars from the g2g transfers. I may need to order more spores to get a fresh set, but I just invested money in that and don't have any to buy more atm. What kind of pics would you like to see of the tub? I use 4-5 quart jars in a tub, have a black trash liner, and make sure the spawn is even once I am finished with it. I have millipore tape over the holes to allow for FAE. The only thing I could think of is the tub did get quite a bit of condensation, but I turned the fan onto it and that dissipated overnight.


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OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 13 days, 2 hours
Re: Trich contamination issues, HELP PLS [Re: smalldaddy]
    #26911673 - 09/01/20 11:06 AM (3 years, 5 months ago)

Well theres your problem.

Spores to grain arent a guarantee. I imagine you had a good lucky streak in the old house.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineSpirit-Crusher
DOOM HIPPIE


Registered: 04/24/19
Posts: 358
Loc: PNW
Last seen: 1 month, 2 days
Re: Trich contamination issues, HELP PLS [Re: The Mycologist]
    #26911744 - 09/01/20 11:46 AM (3 years, 5 months ago)

Quote:

The Mycologist said:
Well theres your problem.

Spores to grain arent a guarantee. I imagine you had a good lucky streak in the old house.



THIS


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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 2,872
Last seen: 5 hours, 53 minutes
Re: Trich contamination issues, HELP PLS [Re: Spirit-Crusher]
    #26911753 - 09/01/20 11:50 AM (3 years, 5 months ago)

its your lack of hygiene, you are to say, on the subatomic level,
Far too dirty, and need to go clean yourself throughly with soap

i recommend increasing the amount of showers per day, using shampoo on all your body hair, even the sex hair, and oil your forearms up with moisture cream real good up before doing the next SAB sterile work

wash up fam


Edited by smalltalk_canceled (09/01/20 11:52 AM)


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Offlinesmalldaddy
Mushroom Enthusiast

Registered: 12/03/19
Posts: 5
Loc: United States
Last seen: 3 years, 7 hours
Re: Trich contamination issues, HELP PLS [Re: The Mycologist]
    #26913713 - 09/02/20 12:40 PM (3 years, 5 months ago)

Thank you for the help! After discussing my spore source with a couple friends who grow, I figured out where I was getting them is not a consistent, reliable source. I'm glad this is the issue, as it means I was probably doing everything else right.


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InvisibleNigel Stephens
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Registered: 02/21/20
Posts: 103
Re: Trich contamination issues, HELP PLS [Re: smalldaddy]
    #26913733 - 09/02/20 12:56 PM (3 years, 5 months ago)

Quote:

smalldaddy said:
Thank you for the help! After discussing my spore source with a couple friends who grow, I figured out where I was getting them is not a consistent, reliable source. I'm glad this is the issue, as it means I was probably doing everything else right.




Everything else right except putting spores to grain. Stop that! Naughty mycologist. Bad


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OfflineYour Dudeness
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Registered: 11/10/18
Posts: 189
Last seen: 10 months, 11 days
Re: Trich contamination issues, HELP PLS [Re: Nigel Stephens]
    #26913757 - 09/02/20 01:16 PM (3 years, 5 months ago)

I don't know why you all are scared of spore to grain but if your work area ever had trich spores dispersed in air all your subsequent grows will get trich. 

If supplier sold crap gear that's problem #1


--------------------
RR sent me


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Offlinenormalperson
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Registered: 10/31/19
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Re: Trich contamination issues, HELP PLS [Re: Your Dudeness]
    #26914747 - 09/02/20 11:09 PM (3 years, 5 months ago)

Trich spores are in the air everywhere, no big deal. You,however, are growing them in the same jars as your spawn. How often/when do you shake your jars?


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OfflineForresterM
aspiring sociopath
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Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA Flag
Last seen: 1 month, 11 days
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Re: Trich contamination issues, HELP PLS [Re: Your Dudeness]
    #26914848 - 09/03/20 01:42 AM (3 years, 5 months ago)

Quote:

Your Dudeness said:
I don't know why you all are scared of spore to grain





People don't recommend spores to grain because it's a terrible idea, not because they're afraid.

Quote:

Your Dudeness said:
if your work area ever had trich spores dispersed in air all your subsequent grows will get trich. 





Not true at all.  If you have healthy spawn and substrates they will be fine.  Like mentioned, spores are everywhere, it's the health of your substrate that keeps it safe.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineYour Dudeness
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Re: Trich contamination issues, HELP PLS [Re: Forrester]
    #26914853 - 09/03/20 01:51 AM (3 years, 5 months ago)

According to RR once you go trich you can't go back.  I side with him because the man is from the mountains!  Do you seriously think you could scrub your grow room from trich spores if they were released indoor?  Assuming the air conditioner doesn't suck up the spores through your HVAC system first. 

Spore to grain is a fantastic idea but if your supplier sells crappy syringes I can see why people scoff at the notion.


--------------------
RR sent me


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OfflineForresterM
aspiring sociopath
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Re: Trich contamination issues, HELP PLS [Re: Your Dudeness]
    #26914854 - 09/03/20 02:00 AM (3 years, 5 months ago)

Quote:

Your Dudeness said:
According to RR once you go trich you can't go back.  I side with him because the man is from the mountains!  Do you seriously think you could scrub your grow room from trich spores if they were released indoor?  Assuming the air conditioner doesn't suck up the spores through your HVAC system first. 

Spore to grain is a fantastic idea but if your supplier sells crappy syringes I can see why people scoff at the notion.




I'm not going to debate with you all day because you watched some ancient RR videos and want to go around parroting what he said instead of speaking from experience, but in short:

- I never said anything about scrubbing your room of spores, but I did explain why you didn't need to.

- Again, spore to grain is not a fantastic idea.  No spore prints are 100% clean, since nobody grows mushrooms in 100% sterile conditions, no matter how good your supplier is.

Just because you've had good luck with it doesn't mean it's a good practice.


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineYour Dudeness
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Re: Trich contamination issues, HELP PLS [Re: Forrester]
    #26914874 - 09/03/20 02:57 AM (3 years, 5 months ago)

Did you have Castratikron playing when you wrote that about my man RR?  Think about this.  The insurance company allows up to a certain ppm atmospheric mold reading inside the home.  Hank Hill had to vacate his home due to mold I tell ya h'wat. 

  If some grower releases millions of trich spores from a bad grow into their grow room that's a lot different than the typical ppm levels in a non growing home right?  We agree there are spores all over the earth but someone releasing sporelated trich indoors is gonna have a bad time.  Rock on man!


--------------------
RR sent me


Edited by Your Dudeness (09/03/20 02:57 AM)


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Offlinemr. whothehell
Stranger


Registered: 08/31/19
Posts: 103
Last seen: 1 year, 14 days
Re: Trich contamination issues, HELP PLS [Re: Forrester]
    #26914909 - 09/03/20 03:45 AM (3 years, 5 months ago)

try agar in order an I'll see a difference in your contam rate, but what kind of substrate do you use and how you pasteurize it ?

Not true at all.  If you have healthy spawn and substrates they will be fine.  Like mentioned, spores are everywhere, it's the health of your substrate that keeps it safe.




no man your totally wrong, that not a good idea, personally anytime I'd any contamination, I changed my house if the contam persist in the new one I moved to another city.

spore to grain it's not mycology it's gambling


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Offlinenormalperson
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Re: Trich contamination issues, HELP PLS [Re: Your Dudeness]
    #26916558 - 09/03/20 10:44 PM (3 years, 5 months ago)

you say you inspect your jars, how? the best inspection method is to shake them, How often/when do you shake your jars?


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Offlinemr. whothehell
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Registered: 08/31/19
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Last seen: 1 year, 14 days
Re: Trich contamination issues, HELP PLS [Re: normalperson]
    #26916658 - 09/04/20 12:46 AM (3 years, 5 months ago)

I sake mine when I star to see some vigorous growth, generally 15 days after inculcation. if you sake a jar and the grains do not speared them that's a sing of contam plus if those jars take too long to ricover that's another sign


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Offlineredhandmat
Dude


Registered: 05/09/19
Posts: 463
Last seen: 2 years, 8 months
Re: Trich contamination issues, HELP PLS [Re: mr. whothehell]
    #26916706 - 09/04/20 02:13 AM (3 years, 5 months ago)

Quote:

mr. whothehell said:
I sake mine when I star to see some vigorous growth, generally 15 days after inculcation. if you sake a jar and the grains do not speared them that's a sing of contam plus if those jars take too long to ricover that's another sign




15 days after inoculation?? Damn, are those really clean? Mine are 100% colonized by day 10-15. Seeing signs of good grows by day 15 seems like something is wrong.


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Offlinemr. whothehell
Stranger


Registered: 08/31/19
Posts: 103
Last seen: 1 year, 14 days
Re: Trich contamination issues, HELP PLS [Re: redhandmat]
    #26916825 - 09/04/20 05:57 AM (3 years, 5 months ago)

yes they are I think it depend about the size of the agar wedge that I use, my jars take 3 weeks ti a month to fully colonized


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OfflineGan
Wielder of Narya
Male


Registered: 08/26/19
Posts: 927
Loc: Valinor
Last seen: 6 months, 14 days
Re: Trich contamination issues, HELP PLS [Re: mr. whothehell]
    #26916835 - 09/04/20 06:17 AM (3 years, 5 months ago)

Your using syringe to grain. there's your problem. Case closed. Taking more showers, or using shampoo everywhere, or lubing up your arms won't solve your problem. Agar will. Once you get to agar, then you can start figuring out what other things may need tweaking. Anyone that says syringe to grain is a good technique in this day and age is just lying to themselves and others. 

If you've had trich, that's fine. No reason to go and scrub everything down. Sure, you can if you want. But I've had tubs sporulate before I can remove them and the rest of my tubs flush just fine :shrug: provided I'm working with clean spawn


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