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OfflineCanebrake
Stranger
Registered: 05/01/20
Posts: 96
Last seen: 5 months, 12 days
Tips for cleaning a MS syringe
    #26912383 - 09/01/20 05:01 PM (3 years, 4 months ago)

I got 2 MS syringes of AA+. I put one syringe to BRF cakes and they are colonizing slowly as suspected. Hoping to be able to get some prints or clone something if they produce. The second syringe I put 1 drop onto 10 agar dishes I have. Hoping to try and transfer. After a week they pretty much all look like this.

Do I just keep dropping spores until they take? That fuzz doesn't look like myc to me.


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OfflineMolecularConcept
Old School Back Again
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Registered: 05/24/10
Posts: 147
Loc: Denver, Co
Last seen: 8 months, 29 days
Re: Tips for cleaning a MS syringe [Re: Canebrake]
    #26912391 - 09/01/20 05:06 PM (3 years, 4 months ago)

contaminated syringe. that white on the edge MIGHT be mycelium but im not sure of what Lol


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InvisibleDarkshadow
Waiting In The Shadows


Registered: 06/11/20
Posts: 55
Re: Tips for cleaning a MS syringe [Re: Canebrake]
    #26912629 - 09/01/20 07:23 PM (3 years, 4 months ago)

Looks like you have too much condensation in the dish with pooling water, I would keep trying on other plates you have made also look into getting or making a inoculation loop to spread around a drop from the MS Syringe. c10 has a good guide showing this technique, it can found on the lower part of their post I have linked.

https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=24330957&PHPSESSID=&fpart=1#24330957


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