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OfflineEnkidu
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Cant get conditions right..
    #26912227 - 09/01/20 03:37 PM (3 years, 4 months ago)



Been stressing me out trying to get these shoeboxes to fruit and having 0 luck then this mono starts pinning only to have the pins start to abort ?

I am having so much difficulty right now...

Spawned 16 qts and nothing is producing or showing good signs. Just fucking failing on me.


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InvisibleChRnZN
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Re: Cant get conditions right.. [Re: Enkidu]
    #26912242 - 09/01/20 03:45 PM (3 years, 4 months ago)

I don't see much water. Droplets should be covering the surface. A dunk might help as well. How long since you began?


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: ChRnZN]
    #26912273 - 09/01/20 04:04 PM (3 years, 4 months ago)

I think my camera just sucks because people keep saying it looks dry but id anything i think its too wet.

Its covered in beads of water and i almost thought maybe the water is causing them to abort

I think its been about 2 weeks since spawn


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OfflineHikeadellic
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Re: Cant get conditions right.. [Re: Enkidu]
    #26912287 - 09/01/20 04:17 PM (3 years, 4 months ago)

Maybe too much FAE/too little humidity?


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: Enkidu]
    #26912299 - 09/01/20 04:23 PM (3 years, 4 months ago)



I just took another photo trying to grt better quality

Idk im at a loss.

Idk wtf im doing wrong but i guess its a learning experience..

These tubs have been difficult as fuck for me to get to fruit


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: Hikeadellic]
    #26912306 - 09/01/20 04:25 PM (3 years, 4 months ago)

Quote:

Hikeadellic said:
Maybe too much FAE/too little humidity?





I dont think thats it because the surface is staying wet without me misting

Seems to me like if anything theres not enough air..


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InvisibleChRnZN
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Re: Cant get conditions right.. [Re: Enkidu]
    #26912326 - 09/01/20 04:35 PM (3 years, 4 months ago)

Could be. How many grows have you finished before?


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Offlinejuvec
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Re: Cant get conditions right.. *DELETED* [Re: Enkidu]
    #26912328 - 09/01/20 04:37 PM (3 years, 4 months ago)

Post deleted by juvec

Reason for deletion: D


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Invisiblefilthyknees
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Re: Cant get conditions right.. [Re: juvec]
    #26912332 - 09/01/20 04:40 PM (3 years, 4 months ago)

Try a 1/4inch casing at spawning.


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Offlinegabbk
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Re: Cant get conditions right.. [Re: Enkidu]
    #26912369 - 09/01/20 04:54 PM (3 years, 4 months ago)

I'm pretty sure the spawn was bacterial. All my bets on it.

Last week I had a tub that also was 2 weeks into birth and wasn't giving pins in spite of being 100% colonized for a good week, until it gave up on me turning green (I had a pretty big Trich contam 3 weeks ago that blew up my whole grow room and didn't know until then, when that tub turnt green and had to think why the fuck that was :sad::mad2:)
Maybe your tub won't turn green because your grow room maybe has little Trichoderma spores floating around, and if it's the case, chances are you will get at least some fruits; once, when I began growing, I had a tub that took 21 days from birth to pin. That clearly was when there wasn't the same quantity of Trich spores in my grow room like now, and the mycelium could grow. Took a blue moon but gave something.
By then I was also struggling with bacteria in the spawn but didn't know because I couldn't recognize the visual difference between the bacterial mycelium and the healthy mycelium. And I'm not talking about those black or colored nasty stuff that everyone can tell, but the type of bacteria that 'invades' or hitch-hikes into the mycelium and the only way to clean it is with antibiotic agar.
Let me illustrate what I'm talking about... Here it is a really good pic I took telling the difference. I took the nastiest jar for the photo. You can see some white 'branches' between the mycelium or even against the glass.  That's the bacteria I'm talking about and gotta pay close attention because it can go undercover, specially if you are managing lots of jars at the same time :rofl:. Sometimes it won't be as easy to spot, but hope it helps for the next runs!


Edited by gabbk (09/01/20 05:46 PM)


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: gabbk]
    #26912490 - 09/01/20 05:59 PM (3 years, 4 months ago)

Ive grown lbs over a couple years.

Like 10-20 lbs

But i used the same variety the entire time and it performed great.

New variety for me

Its possible the spawn was bacterial but at least 6 were from agar and the rest g2g


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: Enkidu]
    #26912498 - 09/01/20 06:03 PM (3 years, 4 months ago)

This was my spawn



This was my spawn. 1 pic was after a shale i think and the other was before full colonization

All smelled good

I put some straight verm over some tubs and watered it down, might try with the rest of the tubs

Any ideas on why the fruits i have are aborting ?


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Offlinetryptkaloids
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Re: Cant get conditions right.. [Re: Enkidu]
    #26912557 - 09/01/20 06:40 PM (3 years, 4 months ago)

You sure they are aborting? Maybe they are just slow. What are the temps? How long have all them knots been there?


--------------------
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OfflineEnkidu
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Re: Cant get conditions right.. [Re: tryptkaloids]
    #26912562 - 09/01/20 06:44 PM (3 years, 4 months ago)

Fucking days man. Probably lile 5 days.

Not sure they are aborting but the heads look like theyre turning blue and black..

The temps might be fluctuating a lot. They are not in an ideal location at all.

Im about to go smoke some weed. So much shit going on in my life stressing me the fuck out then having no mushrooms trying to get some in and people waiting and asking..

I shoulda just used some tissue from my old shit and took it to agar. Maybe ill go that route

Its fun to have a challenge but at the same time i would love to just have some fucking mushrooms


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OfflinePreparationH
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Re: Cant get conditions right.. [Re: tryptkaloids]
    #26912565 - 09/01/20 06:46 PM (3 years, 4 months ago)

When did you spawn to bulk?  That looks like it's about to pin and take off like any day dude

Edit: ok 14 days I didn't see that part, I think you're gonna be good, fane it a few times a day of that makes you feel better but I think that thing is about to pop off


Edited by PreparationH (09/01/20 06:48 PM)


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Offlinetryptkaloids
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Re: Cant get conditions right.. [Re: Enkidu]
    #26912568 - 09/01/20 06:50 PM (3 years, 4 months ago)

Give em a few more days. The best thing i have ever done for my conditions is make a proper mono and ditching unmodded ones.
Give em a little more air and make sure you keep an eye on the rh.
Have you ready comebackkid's surface condition write up?
It's gold


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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OfflineMolecularConcept
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Re: Cant get conditions right.. [Re: tryptkaloids]
    #26912679 - 09/01/20 07:52 PM (3 years, 4 months ago)

Dude I see knots all over the place your gonna be fine. Lock your Temps down.  Cubes are easy your just running slow for so.e reason


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Re: Cant get conditions right.. [Re: Enkidu]
    #26912694 - 09/01/20 07:58 PM (3 years, 4 months ago)

What temp is it running at? I think dropping it down to 70F would be helpful. And how's your air flow?  If the box is closed, could you have the lid rest on top of the closed latches so it creates a little gap?  Just make sure to keep the walls and lid well wetted with clean water.


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: Shroomboofer]
    #26916245 - 09/03/20 07:25 PM (3 years, 4 months ago)



Got some aborts and some pink sub and some tiny ass baby shrooms growin


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OfflineHikeadellic
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Re: Cant get conditions right.. [Re: Enkidu]
    #26916436 - 09/03/20 09:08 PM (3 years, 4 months ago)

Tiny ass baby shrooms are the first step to Big Ass super shrooms


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Offlinepesa
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Re: Cant get conditions right.. [Re: Enkidu]
    #26916447 - 09/03/20 09:17 PM (3 years, 4 months ago)

op do you think you got in to that part of gene pool that goes way to well on agar and grains but never fruits? i am just saying happened to me on amazon variety every thing went well till it reach to fruiting stage. nothing after three weeks i gave up toss the entire tray.


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: pesa] * 1
    #26916791 - 09/04/20 05:01 AM (3 years, 4 months ago)

Idk man.. Maybe

That would suck


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Offlinetryptkaloids
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Re: Cant get conditions right.. [Re: Enkidu]
    #26917092 - 09/04/20 09:42 AM (3 years, 4 months ago)

I think that has more to do with conditions than genetics


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Learn the tried and true way to grow mushrooms
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OfflineHikeadellic
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Re: Cant get conditions right.. [Re: tryptkaloids]
    #26917178 - 09/04/20 10:23 AM (3 years, 4 months ago)

I guess the only way to find out would be to pinch some mike of of the grow and put it back on agar, or maybe just get more spores going and hope it was just a genetic fluke :goodluck:


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: Hikeadellic]
    #26917260 - 09/04/20 11:11 AM (3 years, 4 months ago)

I think if i got the conditions right it would be good but i cant get anything dialed in and i only look at it like once a day cause of being busy and what not


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OfflinePreparationH
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Re: Cant get conditions right.. [Re: Enkidu]
    #26917994 - 09/04/20 06:48 PM (3 years, 4 months ago)

See where it goes, it's possible if you do a dunk maybe flush 2 will put out, I've seen it, how was your field capacity?


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: PreparationH]
    #26918659 - 09/05/20 05:33 AM (3 years, 4 months ago)

I thought my field capacity was good

I think my top layer was too dry though


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Offlinetravels_slightly
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Re: Cant get conditions right.. [Re: Enkidu]
    #26918922 - 09/05/20 09:35 AM (3 years, 4 months ago)

I just did a few tubs that looked like this about -4 days to fruit. The only one that failed was a b+ corn:coir/verm 1:1 mix that essentially was exactly the same as it's neighbor, except I decided to keep the tub sealed most of the time on that B+ and everything else I took the tops off entirely to see the difference between good fae and good humidity as far as triggering pins. the B+ looked great while everything else finished first flush... then I finally gave it a wiff... Bacterial destruction. straight mush to the touch but you'd never have known by looking.


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: travels_slightly]
    #26920517 - 09/06/20 06:26 AM (3 years, 4 months ago)

Ive always basically just spawned shoeboxes and left the lid latched and a huge pinset would develop then i would dub it

Always been pretty hands off


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InvisibleFooMan
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Re: Cant get conditions right.. [Re: gabbk]
    #26920726 - 09/06/20 08:59 AM (3 years, 4 months ago)

Quote:

gabbk said:
I took the nastiest jar for the photo. You can see some white 'branches' between the mycelium or even against the glass.  That's the bacteria I'm talking about and gotta pay close attention because it can go undercover, specially if you are managing lots of jars at the same time :rofl:. Sometimes it won't be as easy to spot, but hope it helps for the next runs!






The white branches in that pic on the right is rhizomorphic mycelium, not bacteria. Please don't encourage people to chuck their jars when they see that.


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Offlinetryptkaloids
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Re: Cant get conditions right.. [Re: FooMan]
    #26920734 - 09/06/20 09:02 AM (3 years, 4 months ago)

The random long branches of rhizos pressed up against the glass have been noted as signs of bacteria however.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Re: Cant get conditions right.. [Re: tryptkaloids]
    #26920925 - 09/06/20 10:37 AM (3 years, 4 months ago)

Source?


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Offlinetryptkaloids
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Re: Cant get conditions right.. [Re: FooMan]
    #26921074 - 09/06/20 12:18 PM (3 years, 4 months ago)

It was a discussion several of us had in general discussion years ago. Im not digging it up.
Either way that jar is bacterial


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Edited by tryptkaloids (09/06/20 12:18 PM)


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Offlinegabbk
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Re: Cant get conditions right.. [Re: FooMan]
    #26921155 - 09/06/20 01:07 PM (3 years, 4 months ago)

Thanks for coming by Foo.

I can't agree with you, as I've spawned both of these jars separated and the left one (the one I say it's clean) gave immensely more tidy and stronger flushes, with earlier pins. At the other hand, the one at the right took longer and gave lot less yield (at least a third less).
In fact it's not the first time it occurs to me. I had now a few grows (without knowing or telling the difference in the mycelium) and had tubs growing much better than the other. Both being from the same genetics (not MS).

Its a pity I can't prove it because I didn't record these grows with pictures but only with registering the final numbers and dates in my laptop. But that's my own meandering experience. I don't see the point on sharing nonsense or to chit chat.
When I began I thought it could be stronger mycelium, as it grew so fast, just in a matter of two days. Then I searched and found out blobs were from bacteria. And then found out all these tubs with fast growing blobs came from these jars with "mycelium pressing against the glass", which you say is rhizomorphic. At one point I thought it was maybe rhizomorphic too, but the fact that this particular "rhizomorphic mycelium" was impervious to water weirded me out, as these grows tended to flood pools of water. Eventually I began to pulling out better agar technique without knowing, and the more I got better grows I finally could tell the difference between bacterial and healthy mycelium. I concluded that in MY case that bacterial spawn was carried in the inoculum, the agar. You can see what I'm talking about in the following thread I've made, in which Pasty helped me to understand the issue: Wrinkles on agar?

And aside from that. Just an extra fun note: I saw a post some months ago in reddit, can't recall exactly which sub, in which someone shared a microscopic video (converted to gif) of bacterial activity replicating INSIDE the mycelium. At that moment I understood why some of my grows went better that others.
I screenshoted it with my phone to share it with some friends of mine that were interested on my updates. I know sharing a random photo proves nothing but I'm sure you can find the original post by somehow looking up the image:



I'm not encouraging to throw these bacterial jars. The opposite! They can be spawned and pump some fruitbodies, but know that there will be way less than an optimal spawn. Also, the condition of having an underlying competitor (the bacteria) weakens the fungi and make it more susceptible to contamination.

Just that. I'm just trying to promote better agar technique and the ability to recognize the correct mycelium :grin:.


Edited by gabbk (09/06/20 02:55 PM)


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InvisibleFooMan
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Re: Cant get conditions right.. [Re: tryptkaloids] * 2
    #26922562 - 09/07/20 07:46 AM (3 years, 4 months ago)

Quote:

tryptkaloids said:
It was a discussion several of us had in general discussion years ago. Im not digging it up.
Either way that jar is bacterial




I wasn't aware the council on contaminants had a discussion about it. That settles it then. My bad.


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Offlinejunk_f00d
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Re: Cant get conditions right.. [Re: FooMan]
    #26922884 - 09/07/20 11:50 AM (3 years, 4 months ago)

Quote:

FooMan said:
Quote:

gabbk said:
I took the nastiest jar for the photo. You can see some white 'branches' between the mycelium or even against the glass.  That's the bacteria I'm talking about and gotta pay close attention because it can go undercover, specially if you are managing lots of jars at the same time :rofl:. Sometimes it won't be as easy to spot, but hope it helps for the next runs!






The white branches in that pic on the right is rhizomorphic mycelium, not bacteria. Please don't encourage people to chuck their jars when they see that.



Quote:

FooMan said:
Source?



Quote:

FooMan said:
Quote:

tryptkaloids said:
It was a discussion several of us had in general discussion years ago. Im not digging it up.
Either way that jar is bacterial




I wasn't aware the council on contaminants had a discussion about it. That settles it then. My bad.





:whathesaid:


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: junk_f00d]
    #26923264 - 09/07/20 03:12 PM (3 years, 4 months ago)

Well... Either way..

I tried casing them with verm so im still trying

Will update..

Have some pins and some mushrooms but far far from the half lb i was hoping for..

More like 2g atm...


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OfflineHikeadellic
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Re: Cant get conditions right.. [Re: Enkidu]
    #26923900 - 09/07/20 09:51 PM (3 years, 4 months ago)

Never count your shrooms before they dry


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Re: Cant get conditions right.. [Re: Hikeadellic]
    #26954571 - 09/25/20 02:22 PM (3 years, 4 months ago)

Soo update.

All those tubs failed. Like 2g total harvest.

Replaced almost all of them. Have a mono and 6 shoeboxes.

Paid more attention to my sub prep and my top layer. I think i was maybe a bit rusty and lazy jumping back in.

Updated my mono and used Pasty's tek with the 1/4 inch holes. Seems to be working well

Good surface conditions and starting to pin

Everything this go is looking much better

Some of the shoeboxes are starting to pin too, but nothing really worth sharing yet


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Re: Cant get conditions right.. [Re: Enkidu]
    #26954598 - 09/25/20 02:38 PM (3 years, 4 months ago)

There are times when extreme rhizo sectors flying off grain have been identified as a symptom of bacteria but, the jars above do not qualify. The rhizomorphs need to be leaping uncolonized areas in a disorganized pattern. This seems to be a difficult distinction for people as I’ve had to correct a few people of late on it.


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Offlinetryptkaloids
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Re: Cant get conditions right.. [Re: Pastywhyte]
    #26954635 - 09/25/20 03:00 PM (3 years, 4 months ago)

Idk man, this looks bacterial to me


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Re: Cant get conditions right.. [Re: FooMan]
    #26954640 - 09/25/20 03:02 PM (3 years, 4 months ago)

Quote:

FooMan said:
Quote:

gabbk said:
I took the nastiest jar for the photo. You can see some white 'branches' between the mycelium or even against the glass.  That's the bacteria I'm talking about and gotta pay close attention because it can go undercover, specially if you are managing lots of jars at the same time :rofl:. Sometimes it won't be as easy to spot, but hope it helps for the next runs!






The white branches in that pic on the right is rhizomorphic mycelium, not bacteria. Please don't encourage people to chuck their jars when they see that.




It's certainly not perfect spawn, but the jar on the right just looks a little overgrown to me, that's all.  Some Trusted Regurgitators (TR) can think otherwise, though.


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Re: Cant get conditions right.. [Re: Josex]
    #26954791 - 09/25/20 05:00 PM (3 years, 4 months ago)

You guys are funny

Maybe i shouldnt have bumped this :lol:

I was excited for these new tubs though, looking promising

Thanks for the simple tub tek with the 1/4 in. holes, easy and seems to work much better than unmodified for me

Hopefully i can get a grow that does these RW justice. My GF took a microdose of .2, what she usually takes, said she felt like she was tripping and it was way too strong.

Hoping for a nice cluster to clone and if i can get that it will be my new go to for bulk

I dig the white a lot


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Re: Cant get conditions right.. [Re: Josex]
    #26954799 - 09/25/20 05:04 PM (3 years, 4 months ago)

Quote:

Josex said:
Quote:

FooMan said:
Quote:

gabbk said:
I took the nastiest jar for the photo. You can see some white 'branches' between the mycelium or even against the glass.  That's the bacteria I'm talking about and gotta pay close attention because it can go undercover, specially if you are managing lots of jars at the same time :rofl:. Sometimes it won't be as easy to spot, but hope it helps for the next runs!






The white branches in that pic on the right is rhizomorphic mycelium, not bacteria. Please don't encourage people to chuck their jars when they see that.




It's certainly not perfect spawn, but the jar on the right just looks a little overgrown to me, that's all.  Some Trusted Regurgitators (TR) can think otherwise, though.




When I have jars that look like the one on the right they're always trouble.


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Offlinetryptkaloids
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Re: Cant get conditions right.. [Re: Stipe-n Cap]
    #26955443 - 09/26/20 02:10 AM (3 years, 4 months ago)

I've had enough bacterial spawn to know that jar is bacterial.
The rhizos aside its a bit gooey in the corners
Id still spawn it tho.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineEnkidu
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Re: Cant get conditions right.. [Re: tryptkaloids]
    #26960706 - 09/29/20 05:01 PM (3 years, 3 months ago)



Well its better than the last round..

Seems to be wanting to blob up on me for some reason like the last try was doing

Making some agar right now, think im going to try and take a tissue sample from the cluster to the right in the mono


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Re: Cant get conditions right.. [Re: Enkidu]
    #26963996 - 10/01/20 02:26 PM (3 years, 3 months ago)



Definitely happy with this tub, looks like its going to give me a decent harvest

Probably wait till tomorrow and take a clone


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InvisiblePastywhyteMDiscord
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Re: Cant get conditions right.. [Re: Enkidu]
    #26964028 - 10/01/20 02:39 PM (3 years, 3 months ago)

I can’t see the whole tub configuration but it looks like much happier surface conditions. That’s going to be a nice flush.


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Re: Cant get conditions right.. [Re: Pastywhyte]
    #26964086 - 10/01/20 03:06 PM (3 years, 3 months ago)

Yeah this time around was a lot better.

Think it came down to my fucking up the sub prep and modifying the tub with those 1/4 in. holes

Much thanks to ya for your contribution :hatsoff:

Somehow made its way to me. And these guys will wind up in others hands too

Im excited to see how a clone from a cluster might perform.

Theres some nice clusters that popped up and i really dig the way these guys look and grow

My gf seemed to think they were potent as well

Seems like an overall winner


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