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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Spinoff from another thread - germinating stubborn spores
    #26911651 - 09/01/20 10:52 AM (3 years, 4 months ago)

Hey Everyone.

I have a bunch of prints that are incredibly stubborn but i dont want to give up on them yet.

Tried numerous times on PDYA carbon agar to germinate them most are incredibly contaminated and some are starting to look like "something" after a month. Tiny white fuzzy specks. Not large enough to know exactly whats going on yet.

I have also made some transfers from a clean plates (softer PDA) to the agar and they look "dry". Perhaps the carbon agar is to hard?

Anyway, i read on another thread that perhaps BRF Tek right be the way to go.

Thoughts?


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OfflineThe Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 29 days, 8 hours
Re: Spinoff from another thread - germinating stubborn spores [Re: maxmush]
    #26911652 - 09/01/20 10:53 AM (3 years, 4 months ago)

I have also heard of people putting a rubber band on the syringe plunger to force hydration into the spores.


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"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Invisiblealaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: Spinoff from another thread - germinating stubborn spores [Re: The Mycologist]
    #26911827 - 09/01/20 12:19 PM (3 years, 4 months ago)

Brf is a great tool for this,  indeed.  You can either make some brf pucks/prepours (just a paste of brf and water cooked until almost too thick to pour then tapped on a table/hand/counter/whatever lightly until flat-ish; or make some standard PF cakes and a syringe then after you get growth,  gently remove cake in SAB to snag a piece of myc.  Either way brf is king for cleaning up absolute trash spores.  If the issue was getting anything at all to germinate, just making a syringe and letting it sit at room temperature for a few days and trying either of the above is the way to go.


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"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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