Home | Community | Message Board

NorthSpore.com BOOMR Bag!
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Jump to first unread post Pages: < Back | 1 | 2 | 3  [ show all ]
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 22 hours, 35 minutes
Trusted Cultivator
Re: Petri dishes keep contaminating [Re: plutoniusspasticus]
    #26911371 - 09/01/20 08:08 AM (3 years, 5 months ago)

It’s gotta be either pouring technique or air can get into whatever you’re using as a plate.

If you have plastic petris the best method I’ve found is to take a stack of five and lift up everything but the bottom dish and pour the agar in, then set the stack back down and pick up the next one and do it again working your way up. Once I do the first five I stack more on top. I used to just start with a stack of 15 but it’s a pain in the ass lifting that many to do the first ones.


--------------------
LAGM2020


Extras: Filter Print Post Top
Offlineplutoniusspasticus
Stranger
Registered: 08/01/20
Posts: 61
Last seen: 6 months, 18 days
Re: Petri dishes keep contaminating [Re: A.k.a]
    #26911586 - 09/01/20 10:12 AM (3 years, 5 months ago)

Quote:

A.k.a said:
It’s gotta be either pouring technique or air can get into whatever you’re using as a plate.

If you have plastic petris the best method I’ve found is to take a stack of five and lift up everything but the bottom dish and pour the agar in, then set the stack back down and pick up the next one and do it again working your way up. Once I do the first five I stack more on top. I used to just start with a stack of 15 but it’s a pain in the ass lifting that many to do the first ones.




I've used a bunch of different containers i.e. glass petris,jars and plastic petris. There doesn't seem to be a difference with contamination so I don't think it's about air getting in.

I've tried your technique in the past which was the time it ended up 100% contam. Tbh, It seems strange that it would be my technique either considering the relatively lazy technique I've seen on youtube.


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,647
Loc: Exact Center
Last seen: 14 hours, 23 minutes
Trusted Cultivator
Re: Petri dishes keep contaminating [Re: plutoniusspasticus]
    #26911667 - 09/01/20 11:03 AM (3 years, 5 months ago)

Quote:

plutoniusspasticus said:
I've determined the problem isn't my pc because I make no pour jars 100% every time. I mist once and wait for 15-20 minutes for it to settle. For some reason I'm only getting around 40% success rate with my pours. Should I upload a video of me pouring to check my technique?
I'm really struggling to find the problem.



Would definately be a good idea

Check the filters on your no-pour


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3  [ show all ]


Similar ThreadsPosterViewsRepliesLast post
* Re: i this a picture of psilocybe mexicana fruiting in a petri dish? mycofile 4,752 10 02/05/00 11:45 AM
by Mycelium5150
* Petri dishes and sterile procedure firsttimer101 1,306 5 01/30/05 09:19 PM
by ATWAR
* Pour agar into petri dishes before pc angelko 6,790 5 05/18/03 05:05 PM
by Prisoner#1
* pinheads on petri dish Senovio 1,713 7 07/27/03 04:53 PM
by Sev
* Some of my first petri dishes ever :) kykeon 3,129 7 10/26/02 12:24 AM
by kykeon
* petri dish sterilization ThumpaCap 13,049 18 03/17/16 06:59 AM
by Supalemonhaze
* How is the best way to pressure cook Petri Dishes?
( 1 2 all )
SafeHaven 15,886 20 12/09/23 10:27 PM
by vazap
* Petri dish condensation firsttimer101 1,030 3 01/30/05 04:11 PM
by scatmanrav

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
644 topic views. 34 members, 183 guests and 74 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.006 seconds on 13 queries.