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Offlinemanic231
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Registered: 05/11/04
Posts: 13
Last seen: 13 years, 3 months
Karo Tek Concerns
    #2691119 - 05/17/04 02:17 PM (14 years, 9 days ago)

I have been reading a lot latly about the Karo Tek, and it seems like quite frankly the greatest thing to come to growers since the PF TEK. I have however one concern. Many growers have stated that although growing with a clone is faster than with spores, repeated cloning eventually leads to the deteriorization of the strain. Basically, the question is, since you can innoculate another Karo jar with the mycilia from an already colonized Karo jar, does that lead to the deterioration of the strain like cloning, or can one spore syringe theoretically produce an unlimited amount of mycelia using the Karo Tek repeatedly. Any comments are appreciated as always.


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OfflineJiggly
still learnin'
Registered: 05/15/04
Posts: 92
Loc: CT, USA
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Re: Karo Tek Concerns [Re: manic231]
    #2691188 - 05/17/04 02:41 PM (14 years, 9 days ago)

im not sure because im a noob... pathetic, i know, but just make a spore syringe from the shrooms that grow, and use those spores to make more karo. if ur not sure how to make a spore syringe


Edited by Jiggly (05/17/04 02:44 PM)


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OfflineIfIWereARichMan
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Registered: 10/27/03
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Re: Karo Tek Concerns [Re: Jiggly]
    #2691567 - 05/17/04 04:02 PM (14 years, 9 days ago)

I think Stamets wrote something about senescence occurring after 3 generations. So you innoculate a single karo jar with spores. The mycelium is then used to innoculate a dozen cakes, each of which are first generations. Then, you use a bit of mycelium from one cake to knock up a dozen more cakes. Generation 2. If you were to repeat the process, you'd be at the 3rd.


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Offlinemanic231
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Re: Karo Tek Concerns [Re: IfIWereARichMan]
    #2691622 - 05/17/04 04:15 PM (14 years, 9 days ago)

What I mean is, can you use the mycelia from one Karo to innoculate another without having to worry about the genetic break down?


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Offlinehomelessheathen
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Registered: 01/29/04
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Re: Karo Tek Concerns [Re: manic231]
    #2693279 - 05/17/04 10:36 PM (14 years, 9 days ago)

I think to reset the genetic clock, you have to start over from a spore print. it could be an old one, or a new one from a recent batch. or even the same original spore syringe if it's been stored well and hasn't gotten stale.


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OfflineHrethic
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Registered: 01/06/04
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Re: Karo Tek Concerns [Re: manic231]
    #2693332 - 05/17/04 10:45 PM (14 years, 9 days ago)

Run a search for it. I know i've read something like that here recently. :mushroom2:


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OfflineTodcasil
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Re: Karo Tek Concerns [Re: manic231]
    #2693595 - 05/17/04 11:42 PM (14 years, 9 days ago)

i see what you are saying... you make a batch of karo mycelia...  you innoc a few jars but have leftover mycelia.

your qeustion: can i keep using the mycelia?

my answer: yes you can :thumbup:

or perhaps your question is can you make another batch of karo with some mycelia from the first? yes you can!

but id stick with the three generation rule of thumb that was stated by IfIWasARichMan from stamets. (as far as three tubs of karo mycelia which is a lot of syringes worth).

if those werent your Qs im sorry i misunderstood!

also IMO i dont think the genetic breakdown would happen as fast from the method you state, so just try it until something wierd happens... genetic breakdown isnth the end of the world, just a botched grow, but you will learn through the great teacher of experience : )


--------------------
Men look at themselves and they see flawed humans, we look at women and we see perfect
GODDESSES
Women look at themselves and they seem utterly human, when looking at men they see proud
GODS.


~Casil



:cactus:


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Offlinemanic231
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Re: Karo Tek Concerns [Re: Todcasil]
    #2695411 - 05/18/04 11:44 AM (14 years, 8 days ago)

Thank you guys so much, and yea that was pretty much my question.


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