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Offlineshorn253
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Registered: 04/24/18
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Storing Master Agar Plates vacuum sealed?
    #26908521 - 08/30/20 06:13 PM (3 years, 5 months ago)

So I realize that mycelium requires some air exchange however the only fridge access I have is my food fridge. There for I would need to double vacuum seal the plates loosely before storing in fridge to avoid contam. I find it hard to believe that the mycelium would need more air than what is in the tray to survive. (Once plates are fully colonized I'm speaking about). It seems that to avoid contam with Petri dishes, you need to basically seal off the edges anyway and I've done so with plain duct tape many times with no issues. Leading me to believe I would be ok to vacuum seal the plates for contam protection in the fridge perhaps with a bit of air left in the bag. Any advice is welcome , thank you!


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Offlineverum subsequentis
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Re: Storing Master Agar Plates vacuum sealed? [Re: shorn253]
    #26908554 - 08/30/20 06:26 PM (3 years, 5 months ago)

My preferred method is to clean with iso and vac seal in front of the flow hood. The myc isn't really growing when in cold storage and therefore does not breathe much.

Also, duct tape is a horrible way to seal petris. It works but is a pain in the ass to work with. Look into glad cling wrap or parafilm. I use glad but some of the fuckers around here still thing parafilm is better (they're wrong).


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InvisibleSmartattack
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Re: Storing Master Agar Plates vacuum sealed? [Re: verum subsequentis]
    #26908671 - 08/30/20 07:47 PM (3 years, 5 months ago)

Quote:

verum subsequentis said:
My preferred method is to clean with iso and vac seal in front of the flow hood. The myc isn't really growing when in cold storage and therefore does not breathe much.

Also, duct tape is a horrible way to seal petris. It works but is a pain in the ass to work with. Look into glad cling wrap or parafilm. I use glad but some of the fuckers around here still thing parafilm is better (they're wrong).





But thats what labs use, It has to be superior.

:lolsy:


--------------------
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Offlineverum subsequentis
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Re: Storing Master Agar Plates vacuum sealed? [Re: Smartattack]
    #26908766 - 08/30/20 08:58 PM (3 years, 5 months ago)

right. ha.
Dude, your planet of the apes globe is one of the coolest things i've ever seen.... and i've seen some shit. good work


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Offlinetryptkaloids
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Re: Storing Master Agar Plates vacuum sealed? [Re: verum subsequentis]
    #26908847 - 08/30/20 10:02 PM (3 years, 5 months ago)

Yea that's pretty fuckin badass :rockon:


--------------------
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InvisibleLotKid
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Re: Storing Master Agar Plates vacuum sealed? [Re: Smartattack]
    #26908880 - 08/30/20 10:32 PM (3 years, 5 months ago)

Quote:

Smartattack said:
Quote:

verum subsequentis said:
My preferred method is to clean with iso and vac seal in front of the flow hood. The myc isn't really growing when in cold storage and therefore does not breathe much.

Also, duct tape is a horrible way to seal petris. It works but is a pain in the ass to work with. Look into glad cling wrap or parafilm. I use glad but some of the fuckers around here still thing parafilm is better (they're wrong).





But thats what labs use, It has to be superior.

:lolsy:



Smart fella, there.

#ParafilmGang
:aliendance:
:goodburger:


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Offlineverum subsequentis
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Re: Storing Master Agar Plates vacuum sealed? [Re: LotKid]
    #26908889 - 08/30/20 10:39 PM (3 years, 5 months ago)

:shitty:


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InvisibleLotKid
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Re: Storing Master Agar Plates vacuum sealed? [Re: verum subsequentis]
    #26908891 - 08/30/20 10:42 PM (3 years, 5 months ago)

:wonka:


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InvisibleSmartattack
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Re: Storing Master Agar Plates vacuum sealed? [Re: LotKid]
    #26910301 - 08/31/20 05:38 PM (3 years, 5 months ago)

I guess I understand it for those who have a few plates that they gingerly wrap and covet like gold in a museum showcase. Im a vendor of plates online and have to wrap hundreds. I can do a couple hundred per hour no problem with cling tight. Also invisible, reliable and very easy to undo for the end user. Not to mention a $4.00 roll will do around 1300 plates. My mind is un-swayable here.

Ill play with some para on my own plates again soon just to see if I can force myself to catch the sickness.


--------------------
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* :thumbup: Planet of the APES:thumbup:
 
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Offlineverum subsequentis
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Re: Storing Master Agar Plates vacuum sealed? [Re: Smartattack]
    #26910311 - 08/31/20 05:41 PM (3 years, 5 months ago)

I tried. And hated it.
And tried again, and hated it.
And tried again and.... decided to just talk shit to lotkid for the rest of my life.
#GladI'mNotRetarded


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InvisibleSmartattack
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Re: Storing Master Agar Plates vacuum sealed? [Re: verum subsequentis]
    #26910330 - 08/31/20 05:44 PM (3 years, 5 months ago)

HAHA. Im gunna put myself on your 4 step program.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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InvisibleLotKid
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Re: Storing Master Agar Plates vacuum sealed? [Re: Smartattack]
    #26910426 - 08/31/20 06:20 PM (3 years, 5 months ago)

If i got plates from a vendor and they were wrapped in cling wrap... i wouldnt take that vendor seriously.

This is how big a piece i use to wrap a plate...



Thats a fuck ton of plates from a single roll.


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InvisibleSmartattack
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Re: Storing Master Agar Plates vacuum sealed? [Re: LotKid]
    #26910438 - 08/31/20 06:23 PM (3 years, 5 months ago)

Luckily there are a lot of folks who aren't you. :shrug:

Most of the folks I sell to care about whether or not they are clean as opposed brand of material used on the edge.


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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InvisibleSmartattack
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Re: Storing Master Agar Plates vacuum sealed? [Re: Smartattack]
    #26910442 - 08/31/20 06:25 PM (3 years, 5 months ago)

I also cut mine on my lathe with a razor attachment into a dozen rolls 3/4" width. Maybe your idea of a cling wrapped dish is when people wrap a 12" square around the plate?


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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InvisibleLotKid
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Re: Storing Master Agar Plates vacuum sealed? [Re: Smartattack]
    #26910450 - 08/31/20 06:28 PM (3 years, 5 months ago)

Quote:

Smartattack said:
Luckily there are a lot of folks who aren't you. :shrug:

Most of the folks I sell to care about whether or not they are clean as opposed brand of material used on the edge.



The world couldnt handle more me. :grin:


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InvisibleSmartattack
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Re: Storing Master Agar Plates vacuum sealed? [Re: LotKid]
    #26910454 - 08/31/20 06:30 PM (3 years, 5 months ago)

You are not alone there.

Oh by the way O.P, sorry about your thread.

:judyfacepalm:


--------------------
* Smarts videos :teacher:
* :thumbup: Planet of the APES:thumbup:
 
I'm a fungal white supremacist.


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InvisibleLotKid
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Re: Storing Master Agar Plates vacuum sealed? [Re: Smartattack]
    #26910458 - 08/31/20 06:32 PM (3 years, 5 months ago)

:jacked:


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Offlineverum subsequentis
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Re: Storing Master Agar Plates vacuum sealed? [Re: LotKid]
    #26910483 - 08/31/20 06:47 PM (3 years, 5 months ago)

Judging a plate by what it's wrapped in is just.... well

#GladI'mNotRetarded


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Offlinejcm4620
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Re: Storing Master Agar Plates vacuum sealed? [Re: verum subsequentis]
    #26910492 - 08/31/20 06:52 PM (3 years, 5 months ago)

Quote:

verum subsequentis said:
right. ha.
Dude, your planet of the apes globe is one of the coolest things i've ever seen.... and i've seen some shit. good work





i always thought it looked like a giant covid 😂😂😂


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InvisibleLotKid
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Re: Storing Master Agar Plates vacuum sealed? [Re: jcm4620]
    #26910496 - 08/31/20 06:53 PM (3 years, 5 months ago)

Usually when i get a plate wrapped in cling; the culture needs a good cleaning.


All joking aside. I dont think cling ships as well as para.


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