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lookintolearn
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Registered: 06/02/20
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My first time Agar Adventures! R8 it don't H8 it
#26904702 - 08/28/20 05:54 PM (3 years, 5 months ago) |
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I'm on my way to making some clean agar plates finally! I've been reading alot and trying to learn what is the best time to get these bad boys to grains but I would like some advice on my plates. LOTS of pictures of different one's, just let me know what you think!
Any advice, comments, or concerns are always appreciated on this pursuit of mushy knowledge.
So here is my best looking transfers from GT and Argentinan pins that I pulled from BRF cakes in the SAB. These are T2. I made these onto Holy Grail containers for better viewing than my pastyplates.
Do any of these look good enough to be put onto grain? 
  
  
   
Here are some of my original starter agar plates and a couple of transfer plates. Bare with me I know they are hard to see but I was curious if they still look pretty viable for extra transfers? Alot of them have tomentose growth which i'm not to sure about. Also they have been stored in the fridge to keep from extra growth and I noticed the condensation has them looking rather wet, is this a problem?
  
  
And finally a blooper picture of mycellium seemingly growing right over some big booger contam with no problem The mycellium on this one (not near the contam) still looks really viable, wondering if I should keep for at least one more transfer or toss this guy?
And also as you noticed i'm sure most of these are touching the edge of the plate already, can you still take a fairly decent transfer NOT from the leading edge? I know the edges touching the cup are more prone to being contaminated.

Thanks for looking!
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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Roger Clemency
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Registered: 03/23/20
Posts: 2,005
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Re: My first time Agar Adventures! R8 it don't H8 it [Re: lookintolearn]
#26904717 - 08/28/20 06:03 PM (3 years, 5 months ago) |
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Nice man. All those top plates look pretty good. A few have spots that are concerning, like areas where the myc just isn’t growing but there’s plenty of good looking myc around to transfer.
As far as going to grain I say F yeah. I wouldn’t LI any of them or anything that uses a whole plate until you’re absolutely sure it’s clean but if T1 or T2 look clean I’ll throw a few wedges to grain while I’m doing transfers to ensure whole clean plates for LI.
Then you’ll end up with some random grain jars to mess with.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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lookintolearn
Stranger becoming Strangest



Registered: 06/02/20
Posts: 574
Loc: Up in the Cut
Last seen: 2 months, 6 days
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Re: My first time Agar Adventures! R8 it don't H8 it [Re: Roger Clemency]
#26904732 - 08/28/20 06:09 PM (3 years, 5 months ago) |
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Thanks buddy! I'm glad I am getting this down finally. Not perfect by no means but I have an understanding at least so far. I'm thinking part of the reason the myc isn't growing uniform is my cuts for transfers have been.. less than perfect to say the least. It sticks alot of the time to the xacto knife i use so a little struggle putting it mycellium down first. I actually bought a leather punch tool as par D3's transfer tek and i'm going to be trying that later tonight I think!
But I do see some of those spots that look sketchy 
My plan is to get them clean enough to do Munch's blenderless route, I was hoping maybe one of them was good enough to try that but you're right I should probably get at least one or two more transfers just to make sure. Don't want to fuck up 5-10 jars because of some ninja contams.
How big of wedges would you say to be adequate for a quart size oat jar?
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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Roger Clemency
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Registered: 03/23/20
Posts: 2,005
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Re: My first time Agar Adventures! R8 it don't H8 it [Re: lookintolearn]
#26905844 - 08/29/20 11:10 AM (3 years, 5 months ago) |
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So I can’t see as clearly as you irl obviously but if it was me I might risk LI with plates #1,6,8 right now. It just depends on the little things.
The first time I tried Blenderless LI I had a T3 plate ready, when I went to actually do it I noticed the wedge was bruising and it was worrying me so the only other full plate I had was a germination plate. I shook that thing up and poured to qts. It worked out well and I got at least first flush with everything, a few tam’d out after that but it was all good. So stuff can work unexpectedly...sometimes ; )
When I do wedges I like 3 of them, each about the size of a dime if it were triangular.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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lookintolearn
Stranger becoming Strangest



Registered: 06/02/20
Posts: 574
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Re: My first time Agar Adventures! R8 it don't H8 it [Re: Roger Clemency]
#26907001 - 08/30/20 12:35 AM (3 years, 5 months ago) |
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I'm going to give it a shot here in a couple days, I'm pc'ing 10 quarts of oats at the moment and probably tomorrow or monday I'm going to try LI with maybe 5 of them, and do some wedges in the others and see how it goes. Just a little test
Will take transfers from them before hand just in case they work out well like you suggested. I'm hoping the test run goes good as it will be my first attempt at using grains as well as agar on anything Excited and scared at the same time haha.
And you use 3 wedges per jar? Or just take three wedges from the agar in general?
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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Roger Clemency
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Registered: 03/23/20
Posts: 2,005
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Re: My first time Agar Adventures! R8 it don't H8 it [Re: lookintolearn]
#26907446 - 08/30/20 10:29 AM (3 years, 5 months ago) |
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I do 3 in a single jar if there’s enough clean myc for it.
Good luck on your journey. Along with using a germ plate for my first LI I also used an unmodded metal lid, I figured it’d be fine since people can vegetables all the time and the jars just seal up nicely but not too crazy. Well after the pressure dropped on PC I took the jar of sterile water out, still real hot, and the water started bubbling like it was boiling. I left it like that for a min or so but it wasn’t stopping so I set the jar back in the hot PC water and that stopped it but I think that bubbling somehow created a super vacuum.
So the lid ended up stuck on so hard there was no way to pry it off with my fingers, pliers, ghetto crow bars, or by shoving knife and scalpel blades under it. I ended up sterilizing a real thin drill bit and then just drilling a hole in the lid to relieve the pressure. The jar was in my SAB, I was drilling through my arm hole.
I heard all the air suck in and figured it was toast but I was trying no matter what. I put a damn bandaid over the hole since that was the only sterile kinda thing I had.
So I dropped the agar puck in and shook the jar, holding onto the bandaid area. Water was sloshing out all over and I was convinced of epic mold in jars but I poured like 20ml a jar and those Beatrice colonized in 10 days and eventually turned into these 
You should always try to be super clean but shit can work sometimes lol. You just don’t wanna be depending on shit working.
I think you’d have a decent chance with a few of those plateses.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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lookintolearn
Stranger becoming Strangest



Registered: 06/02/20
Posts: 574
Loc: Up in the Cut
Last seen: 2 months, 6 days
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Re: My first time Agar Adventures! R8 it don't H8 it [Re: Roger Clemency]
#26907904 - 08/30/20 01:22 PM (3 years, 5 months ago) |
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Thanks for the clarification, If I put wedges in 5 jars (15 wedges total) do you think I should do each of those jars separately since they will be from different agar? (But same type of mush, Argentinian) Or since they are same type it would be fine to combine? I was thinking 1 jar per 20qt container with those. And then the 5 with LI I would try one big 66qt monotub.
I appreciate it, the journey has been a fun one for sure so far. I love the mush hobby and the culture that comes with it. Everyone (almost) has been so great on here and I love the information sharing that comes along with it.
That story is great and makes me feel a little better about my soon to be attempts on this, I can't believe it worked even after you putting a hole in the sterile water jar that sucked air in! That's crazy but hey everything else must have been on point because those are some very nice tubs considering it to be a test haha
I always try to get my sterile procedure down, I still have lots of improvement (I suck at getting wedges off the xacto knife all the time, sometimes wave my hand over agar by accident )
But again, thanks for the advice Roger you've been a big help on a lot of my questions i've had, excited to get this grain game going!
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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Roger Clemency
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Registered: 03/23/20
Posts: 2,005
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Re: My first time Agar Adventures! R8 it don't H8 it [Re: lookintolearn]
#26908122 - 08/30/20 03:06 PM (3 years, 5 months ago) |
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No problem man, the more people growing mush the better spread the fungus, inoculate the minds.
You can mix the jars from different plates, each jar has many strains in it already anyway if it’s MS. And I would probably use more than 1 for each 20qt. I use 1 qt for each 6qt shoe box with a 1:1.5 spawn to sub ratio.
I did a 16qt with 2 qts of sketchy spawn. I wanted 3 but the last was too obviously gone. It still tam’d out first flush but I like how it was going. 2-3 qts is perfect I think for a tub that size. 
I have trouble with wedges sometimes as well. I’ve often just used the lip of the jar or the lid itself. I think that’s the lesser of two evils when the other is roughing up the air trying to shake the wedge off lol.
-------------------- Sour grapes, sweet revenge Heaven starts right where hell ends
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lookintolearn
Stranger becoming Strangest



Registered: 06/02/20
Posts: 574
Loc: Up in the Cut
Last seen: 2 months, 6 days
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Re: My first time Agar Adventures! R8 it don't H8 it [Re: Roger Clemency]
#26908929 - 08/30/20 11:38 PM (3 years, 5 months ago) |
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Agreed, everyone should have access to these lovely little things 
All my agar plates so far have not come from syringe, the mushrooms that I got pins off of did however. The agar has been made from pins in BRF jars that I have transferred a few times. Did it that way so I knew they would be viable But did come from MS in a way, I guess?
And sounds good I'm thinking i'll shoot for 2 jars per 20qt and see what happens as a test, just to see how reliable these agar can be.
Yeah i'm going to attempt to scoop the agar wedge rather than poke it and see if that will help me in my attempts. I got my sterile water cooling down now so will be trying it out here soon!
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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