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Offlinedothedew69
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This doesn’t look right to me...
    #26906531 - 08/29/20 07:26 PM (3 years, 9 months ago)

I’ve been playing with lc in addition to grain with me and I’ve isolated this one jar because it just doesn’t look right. Gotta be some sort of contam and probably need to get rid of it ASAP.



Then I have this one grain jar that doesn’t look right as well, pictures are really hard because it has built up a lot of condensation but doesn’t look like typical mycelium. Gotta excuse my noob ness as I get used to cultivating again.


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Offlineblindsighted
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Re: This doesn’t look right to me... [Re: dothedew69]
    #26906555 - 08/29/20 07:47 PM (3 years, 9 months ago)

Grain looks like bacteria and to much moisture and maybe some other stuff. How was the liquid culture made and what did u use for incoculation and procedures?


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OfflineSpore211215
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Re: This doesn’t look right to me... [Re: blindsighted]
    #26906597 - 08/29/20 08:11 PM (3 years, 9 months ago)

That grain needs to be trashed ASAP, and idk much about LC but imo thats trash too. Get a second opinion on the LC but that grain is 100% bad bad no good

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Offlinedothedew69
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Re: This doesn’t look right to me... [Re: Spore211215]
    #26906620 - 08/29/20 08:22 PM (3 years, 9 months ago)

Gotcha grain is out the door. I followed Agar’s LC recipe 1 quart of water with 1 table spoon of lme and 1 of dextrose. Inoculated in a sab, swabbed needles with 70% alcohol, heated with propane torch to red, then inoculated In the injection port. I recently got 2 square meters of h14 hepa filter material so I’m going to be swapping out the Polyfil and such. That one jar had a sfd but didn’t realize it was like 20 Microns. Trashing that grain jar right now far in my woods. If I wash it with soap and water is it still good to use or just trash the jar period?

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OfflineSpore211215
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Re: This doesn’t look right to me... [Re: dothedew69]
    #26906630 - 08/29/20 08:26 PM (3 years, 9 months ago)

Oh no you can still use the jar after you wash it bud, just get all that gross shit out of it and rinse it out with some water and soap away from where you work on your shrooms. Ive never seen clean LC that looked like that though so idk. I'm literally in my second ever attempt at LC rn and im only on day 1 so thats all gonna be learning to me

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Offlineblindsighted
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Re: This doesn’t look right to me... [Re: dothedew69]
    #26906636 - 08/29/20 08:29 PM (3 years, 9 months ago)

Boil the jar to kill whatever. Was the lc made from a multispore solution or a wedge? Was the jar sterilized in a pressure cooker? I personally wouldn't ever wipe the needle, just flame sterilize and you should be good. Just squirt out a drop to cool off needle also.


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Offlinedothedew69
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Re: This doesn’t look right to me... [Re: blindsighted]
    #26906642 - 08/29/20 08:32 PM (3 years, 9 months ago)

Got it, boiling the jar already cleaned it with soap and water and mold/mildew killer. As for the lc that was a ms inoculation. The lc was prepped and then put in the lc for 30min at 15-16 psi. And notes I’ll just flame sterilize the needle going forward. Thanks for all your help!

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Offlineblindsighted
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Re: This doesn’t look right to me... [Re: dothedew69]
    #26906647 - 08/29/20 08:37 PM (3 years, 9 months ago)

Maybe longer pressure cook time, and the mutltispore solution is like a 99.999% chance of contamination. Better to do agar wedge transfer or a live tissue needle biopsy transfer.


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OfflineSpore211215
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Re: This doesn’t look right to me... [Re: blindsighted]
    #26906655 - 08/29/20 08:42 PM (3 years, 9 months ago)

Get into agar if you can my dude, its pretty cool and keeps spawn WAYY cleaner

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Offlinedothedew69
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Re: This doesn’t look right to me... [Re: Spore211215]
    #26906901 - 08/29/20 11:17 PM (3 years, 9 months ago)

I just poured 20 sleeves of mea agar about 20 minutes again :laugh: btw after they are poured how long do I have to wait to inoculate with spores after pouring them? I’m also got another 500ml of agar I’m the pressure cookers as we speak. I’m waiting on a box of 500 it should be here this week or so.

Edit: I’m also ordering another one of those flasks should be here in a couple days I want to have one to pour and one to be ready to pour. I just ditched a bunch of grain that was way too wet so I want to prep some rye berry but I don’t want to be watching a PC on an outside burner and another on the kitchen stove lol.

Edited by dothedew69 (08/29/20 11:20 PM)

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OfflineA.k.aM
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Re: This doesn’t look right to me... [Re: dothedew69]
    #26907106 - 08/30/20 05:00 AM (3 years, 9 months ago)

That jar is soaked, either prepped too wet or way too much lc.  Like a small spoonful is plenty for a quart. I read the recommendations but still had to see for myself and anything more than that slows things down even with grain prepped a little dry.


The lc looks like myc got stuck to the wall and grew out. Idk how you made it but I’m not sure what to think of it. The myc looks ok but it also shouldn’t be floating in chunks like that.


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LAGM2020

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OfflineSpore211215
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Re: This doesn’t look right to me... [Re: A.k.a]
    #26907273 - 08/30/20 08:54 AM (3 years, 9 months ago)

Are you doing a pasty plate or hot pour? Either way I'd say just wait till they solidify and give it another 30mins ish and they should be ready to go. Just once they're under like 80ish degrees you should be able to work on em

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Offlinedothedew69
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Re: This doesn’t look right to me... [Re: Spore211215]
    #26907854 - 08/30/20 01:02 PM (3 years, 9 months ago)

I have the agar a few hours to solidify, used like two drops of spore solution and did zig zag.
As for my grains I agree they were way too wet, I’ve been cooking and soaking a shit ton lately to perfect my grains, I have a great set of oats that are dry but moist same with sorghum. Soaking some rye berries now as well, I now understand the concept of moist and dry, learning curve! I’m basically relearning to do this all again after 8-10 years. As far as the LC I’m not sure what’s going on. It floats, it doesn’t break up, it’s white, and now it’s kind of spotted in the middle. HOWEVER, mycelium is definitely growing around it which is so weird. I actually have 4 jars of LC that are coming along very nicely. I tossed out a crap ton of grains in my woods, I’m going to take that LC and just pour it over the grains, take a handful of hpoo and straw mixed up, give it a good swirl with rubber gloves. Who knows something may grow, but it’s 1000 meters from my home lmao and I’m going to bring alcohol to clean the shit out of my gloves and arms after I deal with it

Edit: I’m injecting grain with .5cc of spore solution as well. The grain was just too wet.

Edited by dothedew69 (08/30/20 01:03 PM)

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OfflineYour Dudeness
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Re: This doesn’t look right to me... [Re: dothedew69]
    #26907988 - 08/30/20 02:07 PM (3 years, 9 months ago)

I bet Joe Rogan would offer you $10,000 to eat that jar of grains.  Watch my main man RR video "lets grow mushrooms" grain prep video.  It'll change yer life!


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Offlinedothedew69
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Re: This doesn’t look right to me... [Re: Your Dudeness]
    #26909525 - 08/31/20 11:24 AM (3 years, 9 months ago)

Quote:

Your Dudeness said:
I bet Joe Rogan would offer you $10,000 to eat that jar of grains.  Watch my main man RR video "lets grow mushrooms" grain prep video.  It'll change yer life!




I actually just purchased the video (yeah I know you can find it for free but I support developers) and I’ve been watching it. I used to have it a long time ago but again it’s been long lost. I made a new batch of oats, used bods oat tek but modify it a bit. I put the grain in the pot, added few scoops of gypsum, mixed it very well then strained it, washed, rinsed, did it until the water was clear. Added one more scoop of gypsum and cooked for roughly 20min I kept testing the grains, biting into them to see when they were perfect. Dried them in the Texas sun, mixing every so often got a really dry product but could still tell they were moist. This was my end result:

Much better result than before. I also gave it a short soak about an hour before boiled which I know in the oat tek it doesn’t say to do so, just trying different things. I think these jars will do really well. I have a couple other jars colonizing now. I swapped to plastic lids with air tight seals, injection port put in with rtv, put extra rtv on the underside to fill any gaps. Then I purchased h13 hepa fabric cut them into squares and attached them with rtv to the lids for my filtration. Only thing I noticed was moisture for in through the filter material, very small amount so I ordered USPS tyvek envelopes. Going to cut tyvek squares and rubber and them around the top of the lid then put tin foil around the top which should negate that.

Edited by dothedew69 (08/31/20 11:29 AM)

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OfflineYour Dudeness
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Re: This doesn’t look right to me... [Re: dothedew69]
    #26909758 - 08/31/20 01:16 PM (3 years, 9 months ago)

You can add gypsum during the soak but adding it then straining and rinsing probably wasted gypsum.  You can support RR but I think he moved onto cannabis.  He's probably a millionaire anyway lol.


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Offlinedothedew69
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Re: This doesn’t look right to me... [Re: Your Dudeness]
    #26909771 - 08/31/20 01:24 PM (3 years, 9 months ago)

Lol probably, but I did add gypsum back into the cook so it’s all gravy. I just let it soak with some gypsum, then strained, then added some while I boiled it. The grain is not sticky at all, it’s really nice.

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OfflineYour Dudeness
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Re: This doesn’t look right to me... [Re: dothedew69]
    #26909820 - 08/31/20 01:49 PM (3 years, 9 months ago)

I think RR added gypsum before soaking overnight so it's incorporated inside the grain.  You can't rinse it out that way!  You bite into the oats?  Use yo hands son! Only time I bite into oats is oatmeal for breakfast


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Offlinedothedew69
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Re: This doesn’t look right to me... [Re: Your Dudeness]
    #26909837 - 08/31/20 01:56 PM (3 years, 9 months ago)

Eh when I tried to break them they just bent, they looked good on the outside but white on the inside, um nom nom. Didn’t taste too bad.

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OfflineYour Dudeness
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Re: This doesn’t look right to me... [Re: dothedew69]
    #26909856 - 08/31/20 02:05 PM (3 years, 9 months ago)

I don't have any oat experience except for oatmeal.  With grains they soak up water and expand.  I'd recommend trying grains like rye berries. 

  Your uncle Marsellus Wallace is rich.  You an Oat man Jimmy?  "Oats Nice"


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RR sent me

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