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OfflineMycoWill
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Registered: 01/26/20
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Last seen: 3 years, 3 months
Spotting contamination on agar
    #26902146 - 08/27/20 11:14 AM (3 years, 5 months ago)

Hello all,

I've been running up against it lately with contamination in both my grain bags and tubs. Some bags have been getting a yellow to brown slime on the grains and stall out, I'm assuming it's a bacterial infection. My tubs have been getting trichoderma. I'd say I'm at about a 20-30% contamination rate so obviously I'm doing something wrong. I have noticed it seems to be much more prevelant in some of my strains and some generations of specific strains which makes me think I'm not doing a good job at spotting contamination on my agar plates.

When a plate has nice rhizomorphic mycelium growth (like my aa+ pic) can I assume there's a good chance it's clean?



And when a plate gets fluffy, uneven mycelium growth (like my b+ pic) can I assume the mycelium is struggling and there's some kind of contamination going on in there?

Note: a different clone of my b+ produced quick to grow, nice rhizomorphic mycelium like the aa+ plate so I don't think it's a b+ thing to produce fluffy mycelium.

Thanks for any advice.


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Offline3SIXTY5
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Registered: 03/27/20
Posts: 62
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Re: Spotting contamination on agar [Re: MycoWill]
    #26902223 - 08/27/20 11:58 AM (3 years, 5 months ago)

If you're using a flow hood to do your transfers and you have a decent sterile technique it's pretty unlikely that your dishes are contaminated, are you going, clone --> agar --> grain, or are you doing multiple transfers in between the cloned dish and the grain bags?


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OfflineMycoWill
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Registered: 01/26/20
Posts: 97
Last seen: 3 years, 3 months
Re: Spotting contamination on agar [Re: 3SIXTY5]
    #26903627 - 08/28/20 07:24 AM (3 years, 5 months ago)

Quote:

3SIXTY5 said:
If you're using a flow hood to do your transfers and you have a decent sterile technique it's pretty unlikely that your dishes are contaminated, are you going, clone --> agar --> grain, or are you doing multiple transfers in between the cloned dish and the grain bags?




Well I do follow sterile procedures and have all necessary equipment. So the contamination is coming from somewhere. I go clone to agar, usually do a minimum of 3 transfers then go agar to grain jar and one grain jar will spread out to 4 5lb 3t unicorn bags.


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Invisiblegrownright
Duke of Lizzards
I'm a teapot


Registered: 10/06/14
Posts: 1,030
Loc: Deep South Flag
Re: Spotting contamination on agar [Re: MycoWill]
    #26903701 - 08/28/20 08:22 AM (3 years, 5 months ago)

Quote:

MycoWill said:
When a plate has nice rhizomorphic mycelium growth (like my aa+ pic) can I assume there's a good chance it's clean?





Never assume its clean. Some contams can hide in an agar dish as well, within the mycelium itself. I had a dish recently I had a feeling had some hidden contams, I transferred some other dishes that were taken around the same time and left it alone. 2-3 weeks later the dish was green.

Quote:

MycoWill said:
And when a plate gets fluffy, uneven mycelium growth (like my b+ pic) can I assume the mycelium is struggling and there's some kind of contamination going on in there?

Note: a different clone of my b+ produced quick to grow, nice rhizomorphic mycelium like the aa+ plate so I don't think it's a b+ thing to produce fluffy mycelium.





Any variety can provide tomentose or rhizomorhic mycelium, its all down to genetics. With that being said theres clear bacteria in the upper portions of these two dishes


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