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Mycostotle
Stranger

Registered: 11/11/19
Posts: 36
Last seen: 1 year, 7 months
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Snake venom agar tek?
#26903592 - 08/28/20 06:57 AM (3 years, 4 months ago) |
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Psilocybe semilanceatas growing here again, I need a tek for snake venom agar to attempt hybridization with Cubensis
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grownright
Duke of Lizzards



Registered: 10/06/14
Posts: 1,030
Loc: Deep South
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Re: Snake venom agar tek? [Re: Mycostotle]
#26903707 - 08/28/20 08:25 AM (3 years, 4 months ago) |
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Quote:
RogerRabbit said:
Quote:
I was movin some hay bales and found a 3 ft. rattle snake. So I busted a cap in his ass
I take it you'd rather have rats, mice, and skunks shitting and pissing in your hay? 
You need sterile snake venom if you're going to use it with agar. It's heat sensitive and ruined by autoclaving, so it has to be added as the agar cools below 100F. It's milked under sterile conditions in front of a laminar flow hood, and then freeze dried to a powder, and must be stored at -20C or below until use. It's also about $400 to $500 per gram.
Most strains will readily cross without using venom. It will help with non-compatible strains, and it's also been used to successfully cross different species.
If you want to cross two strains(I'm using 'strain' in the sense it's usually used here, that is naming a print and calling it a strain), simply print one cap right on top of the print left by another cap. By then using the resultant print, it would be nearly impossible to not get several crosses. You'd want to do it on agar and isolate the strains as they differentiate, carefully labeling each one. when you fruit them, you'll be able to tell which is a cross or not, provided you used two distinctly different parents. RR
Quote:
RogerRabbit said: I doubt you'll have success with inter-genus hybrids. RR
Good luck kid. You're looking at a pipe dream. Stick to the basics
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Thomsen



Registered: 09/27/19
Posts: 364
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Re: Snake venom agar tek? [Re: grownright]
#26903714 - 08/28/20 08:30 AM (3 years, 4 months ago) |
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If you manage to grow the liberty cap you're the man. I'd give it a shot with Cantharellus cibarius too if I were you.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,269
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Re: Snake venom agar tek? [Re: Thomsen]
#26903720 - 08/28/20 08:39 AM (3 years, 4 months ago) |
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Here’s a link to a research paper RR posted on hybridization. There’s a lot of stuff in the search function.. just search “snake venom” and click the “posted on” column header to sort by date if you’re not set up for it already
Faht
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