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OfflineKoala
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Aerial mycelium and solution
    #26903447 - 08/28/20 03:30 AM (3 years, 4 months ago)

Dear Friends,

After a lot of searching I couldn't find an answer to this specific question. I know aerial mycelium is a sign of lack of FAE, so that is why my "problem" confuses me.

Situation : I have two monotubs, with in total 10 16mm holes each. Two high on each short side, three low on each long side. The bottom holes have two layers, and the top holes one layer of micropore tape.



As you can see they are next to each other in a well lighted and ventilated room.
Both are showing healthy growth, but one of the two has a lot of aerial mycelium.







So I would say it is because of a lack of FAE but the tub next to it has almost no aerial mycelium.



The only two differences between the tubs is a slight difference in measurements and different genetics ( the tub without aeril myc is a monoculture, the one with AM is a multispore)

So what do you guys think the reason of difference is?

And second question is, how could I solve it? Bigger holes, less tape, flipping lid, etc.?

I would love to hear your thoughts, again thanks in advance!



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InvisibleGoatrider
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Re: Aerial mycelium and solution [Re: Koala]
    #26903451 - 08/28/20 03:49 AM (3 years, 4 months ago)

I believe it`s up to genetics.
With lack of air (you even don`t need that much for colonization)
there would be other issues.
I noticed it mats down as pins form.
Just look for proper surface moisture and you will see pins soon.


                    :cookiemonster:


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Offline3SIXTY5
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Re: Aerial mycelium and solution [Re: Goatrider]
    #26903508 - 08/28/20 05:39 AM (3 years, 4 months ago)

Just Genetics dude, it shouldn't matter whether it has aerial mycelium or not. So I'm assuming this, but you're thinking because the mycelium is reaching up it's looking for more FAE?

Like GoatRider said, as long as your surface conditions are good, you'll see pins soon.


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OfflineA.k.aM
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Re: Aerial mycelium and solution [Re: 3SIXTY5] * 1
    #26903548 - 08/28/20 06:21 AM (3 years, 4 months ago)

Yeah it just grows that way sometimes.

Your monoculture is still probably counted as ms unless it came from a clone. The whole ms/monoculture/isolate terminology is really confusing. I still haven’t found a solid agreed upon definition for monoculture and isolate.

The one agreed upon term seems to be ms meaning anything non clone.


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OfflineKoala
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Re: Aerial mycelium and solution [Re: Goatrider]
    #26905968 - 08/29/20 12:41 PM (3 years, 4 months ago)

Thanks for the response, I'll keep an eye on the surface moisture.

My bet also would have been genetics because the tubs are the same.


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OfflineKoala
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Re: Aerial mycelium and solution [Re: A.k.a]
    #26905975 - 08/29/20 12:46 PM (3 years, 4 months ago)

The monoculture tub is indeed from a clone(tissue sample on agar, agar to grain, grain to substrate) . And the ms tub came from spores on agar, agar to grain, grain to substrate.


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Offline3SIXTY5
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Re: Aerial mycelium and solution [Re: A.k.a]
    #26907800 - 08/30/20 12:30 PM (3 years, 4 months ago)

Tissue clones from a MS grow doesn't make it an isolate, there would still be multiple sets of genetics in the cloned tissue if it came from aMS grow. Maybe we are talking about a difference in definitions here, but an isolated set of genetics would be a monoculture, they're two different terms describing the same thing, a culture with one single set of genetics.


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OfflineKoala
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Re: Aerial mycelium and solution [Re: 3SIXTY5]
    #26909886 - 08/31/20 02:18 PM (3 years, 4 months ago)

Ah ok, learned something again.. I didn't know, one mushrooms is a collection of different genetics.. So it is possible to keep taking tissue samples from fruits that grow from a clone, without getting in to genetic trouble?


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Re: Aerial mycelium and solution [Re: Koala] * 1
    #26910766 - 08/31/20 08:53 PM (3 years, 4 months ago)

Cloning a clone weakens genetics over time,
like grain to grain.


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OfflineA.k.aM
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Re: Aerial mycelium and solution [Re: Goatrider]
    #26910877 - 08/31/20 09:55 PM (3 years, 4 months ago)

You can do it a few times with no problem though. I was told not to clone clones when I started but I’ve seen it done many times since then.


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InvisibleGoatrider
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Re: Aerial mycelium and solution [Re: A.k.a]
    #26911253 - 09/01/20 06:42 AM (3 years, 4 months ago)

Yep, could work for a while.
I remember a guy here doing 5-6 clone-clones in a row without problems.
If we only had a schedule for this  :wink:


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Offline3SIXTY5
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Re: Aerial mycelium and solution [Re: Goatrider]
    #26911322 - 09/01/20 07:36 AM (3 years, 4 months ago)

Quote:

Goatrider said:
I remember a guy here doing 5-6 clone-clones in a row without problems




No shit eh, I havent seen anything like that, pretty interesting. Fungi are just the weirdest little creatures lol.


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OfflineKoala
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Re: Aerial mycelium and solution [Re: A.k.a]
    #26911870 - 09/01/20 12:37 PM (3 years, 4 months ago)

So what would be the safest bet? To take a sporprint after 3 clones from clones, and start from there? Or just keep on going till I see diminishing returns?
Or what is your way of working?


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OfflineKoala
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Re: Aerial mycelium and solution [Re: Goatrider]
    #26911874 - 09/01/20 12:38 PM (3 years, 4 months ago)

And what is yours, Goatrider?


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InvisibleGoatrider
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Re: Aerial mycelium and solution [Re: Koala]
    #26911918 - 09/01/20 12:58 PM (3 years, 4 months ago)

Making a master slant :thumbup:


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OfflineA.k.aM
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Re: Aerial mycelium and solution [Re: Goatrider]
    #26911960 - 09/01/20 01:15 PM (3 years, 4 months ago)

I’ve got so many new varieties to do I haven’t done extensive work on clones yet. I would just mess around and see what happens, find a good clone and keep cloning it until you see consistent diminished returns.


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Re: Aerial mycelium and solution [Re: A.k.a]
    #26911998 - 09/01/20 01:38 PM (3 years, 4 months ago)

Lucky man :wink:
I wish i'd have more space.
Just doing about 5 grows a year :rolleyes:


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OfflineA.k.aM
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Re: Aerial mycelium and solution [Re: Goatrider]
    #26912042 - 09/01/20 01:54 PM (3 years, 4 months ago)

lol the plates take up the most space. I ended up with like 30 different cube prints so I’ve kinda been running each one then taking a clone and prints and moving onto the next print.

You can do a ton of shoeboxes with no room if you stack them up. I had like 20 in a corner at one point. Barely taking up any space.


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InvisibleGoatrider
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Re: Aerial mycelium and solution [Re: A.k.a]
    #26912097 - 09/01/20 02:23 PM (3 years, 4 months ago)

Didn't mean just 5 boxes for 5 grows.
If i do 20 at a time, i'd call it 1 grow.
Perhaps a bit old fashioned my points of view  :rofl:


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OfflineKoala
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Re: Aerial mycelium and solution [Re: A.k.a]
    #26913587 - 09/02/20 11:24 AM (3 years, 4 months ago)

Thanks for the info guys.. I am going to read up on making master slants :thumbup:

A question for you A. K. A
Are your grows from all these different prints, as consistent as your pictures? I was wondering in which degree such grows are determined by genetics or growing conditions. Sure enough those are some isolates/monocultures right?


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OfflineA.k.aM
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Re: Aerial mycelium and solution [Re: Koala] * 1
    #26913598 - 09/02/20 11:33 AM (3 years, 4 months ago)

lol no those pics are my best/favorite ones and then one of my very first grow. I wish they all came out like that.  The PE and the one next to it that’s close up are clones the rest were all from spores.

The lagm link has a bunch of grows I did.

For example here’s a pesa shoebox I did at like 33 days old.


:facepalm::lol:

Easily my worst ever. I think most people would’ve tossed it after a month with no pins but the surface still looked pretty good. It actually did eventually grow some decent size mushrooms a couple weeks later.

These are more of what my average ms shoeboxes look like


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Edited by A.k.a (09/02/20 11:42 AM)


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OfflineKoala
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Re: Aerial mycelium and solution [Re: A.k.a] * 1
    #26913674 - 09/02/20 12:14 PM (3 years, 4 months ago)

Lol.. You are indeed a trooper for keeping that shoebox.

But good to hear, so it is definitely genetics which makes a grow good or great..

What is the reason for you to grow in shoeboxes? Easier to handle or does it give you more shrooms per pound of substrate?

And what spawn ratio you use?
I've got 16 quarts of spawn and I am doubting to do 3, 4 or 5 44 quarts tubs.
Or make it all shoeboxes. What are your thoughts on that?


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OfflineA.k.aM
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Re: Aerial mycelium and solution [Re: Koala] * 1
    #26913745 - 09/02/20 01:07 PM (3 years, 4 months ago)

Just cuz I picked shoeboxes to start with and was originally planning on growing a couple ounces and being done with it. Like I was seriously hyped about the thought of having two ounces in the freezer. :lol:

Also a huge reason was I didn’t have a pc. I used an instant pot and could only do one quart at a time.

Once I got a pc I started doing a lot of underbed tubs. Much larger shrooms for sure.


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OfflineKoala
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Re: Aerial mycelium and solution [Re: A.k.a] * 1
    #26913805 - 09/02/20 01:52 PM (3 years, 4 months ago)

Lol.. The good old days. I'll just stick to the tubs.

Thanks for taking the time to reply! I am pretty good on my way, but it helps a lot to be able to ask someone about all these details.  :takingnotes:


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Re: Aerial mycelium and solution [Re: Koala] * 1
    #28182724 - 02/11/23 05:03 PM (11 months, 10 days ago)

Hey! I know I'm super late to the thread but was hoping you could tell me a little more about how you modified your mason jar lids? I recognize the blob of silicone glue but how does that big hole in the middle work? I'm trying to make a lid that functions like a self healing port but can accommodate the width of a pipette, and it looks like your lid might be capable of something similar?


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#1 Tek
With utmost care and precision,I work to achieve my fungal mission.
Sterilizing all my tools and space,to ensure not a single spore is out of place.
I carefully select the fungal strain and onto the agar, I make it rain.
With a steady hand and gentle touch, I inoculate the plate without a hitch.
Success is sweet when it's pure and true, and my agar plates are a testament too. A job well done, with no contamination, my fungi inoculation, a thing of  admiration.
JK NO
--Bacteriological Virtuoso aka Master of Mold


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