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mind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
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Ascomycete mycelium
#26902671 - 08/27/20 04:31 PM (3 years, 4 months ago) |
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So, I've been trying to find the answer to these questions for a few months now, with no success, and maybe y'all can help me out.
Earlier this year, I found some morels, Morchella importuna, and read something that said that species is one of the better morels to try and cultivate. Operating purely on habit, I did what I usually do with a new mushroom I want to cultivate, and that was to "clone" with a tissue sample on a few agar plates. Obviously, given that morels are ascomycetes, this did not work like it does with basidiomycetes. I'm sure many of y'all here in the advanced forum know about the differences between the life cycles of ascomycetes and basidiomycetes, but a quick recap:
Basidios spend the majority of their life cycle in a dikaryotic state, and when fruit body tissue reverts back to it's vegetative mycelial state, it stays dikaryotic. This is why cloning works, and why the tissue from a mushroom can still produce viable mycelium. Ascos spend most of their life cycle in a monokaryotic state, and only form dikaryons once it's time for fruit body production.
However, what I'm wondering is, once that dikaryon is formed, the ascocarp is produced, and the spores are ejected, what happens to that dikaryotic tissue? Does it revert back into two separate monokaryons, and become vegetative mycelium? Or does the tissue essentially die, given that it accomplished it's goal of reproduction?
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mind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
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tryptkaloids
Learner



Registered: 02/08/15
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I know pretty much nothing on the subject but i assume it produces spores until it gets broken down by something external
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
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Quote:
tryptkaloids said: I know pretty much nothing on the subject but i assume it produces spores until it gets broken down by something external
Yeah I'm sure other fungi/bacteria/molds will break down the fruit body, but the question is whether or not the ascomycete fruit body will revert back to a vegetative mycelium, like a basidiomycete fruit body will.
Any other thoughts on this topic?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
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Last seen: 2 days, 11 hours
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I doubt it.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Loc: Milky way
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Basidiomycetes mycelium doesn't revert back to vegatative growth after the fruit body produces spores. It rots away. Same for ascos. Cloning might inadvertently happen to asco and basidio species by accident in nature from fragmentation of fruit bodies. You can clone morels the same way as basidio species
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mind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
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So does fruit body tissue only revert back to vegetative mycelium when it’s fragmented? I’ve always been under the assumption that you can take any piece of fruit body from a Basidio to clone and create mycelium. Isn’t that how cloning works?
You can clone a morel just like basidios. That’s good to know. But is that resulting mycelium that would come from a cloned morel fruit body dikaryotic or monokaryotic?
Thanks for the response bod
-------------------- Mind's Easy Bag 2 Bag Grain Transfers Endless Sub Tek ...the doll's trying to kill me and the toaster's been laughing at me...
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