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Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures

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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26913467 - 09/02/20 09:51 AM (3 years, 4 months ago)

Something is up with your LC and/or grain prep if you cant get a 6-15cc LC to colonize a 4qt bag.


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Offlinemycopiss
Stranger
Registered: 06/01/19
Posts: 352
Last seen: 1 year, 9 months
Re: Liquid Culture or Liquid Innoculant [Re: maxmush]
    #26913535 - 09/02/20 10:53 AM (3 years, 4 months ago)

It colonized, just slow for LC.  Might be that my grains were too dry. I had wet bags so started prepping a bit more dry.

What i dont understand is how 6-15cc would colonize a 4qt bag at normal speed, when most people use 6-10 cc on 1qt jars.  Wouldnt I have to increase the amount of LI/LC used when doing 4qt vs 1qt?

How much LC Or LI per 1 qt are you using and what are you using LI or LC?



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Invisiblealaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26913577 - 09/02/20 11:18 AM (3 years, 4 months ago)

For both LI and LC I use 4-5cc max per qt.  I prep my grain on the dry side for a reason: I don't want jars fostering bacterial growth and stunted by too much moisture.  Going ham on LI/LC is a good way to get slow colonization.  I'd much,  much rather get quick 25-40% colonization and give a shake to finish it up than dump too much liquid in there and watch it crawl.  Both LI and LC work fine.  As was stated by previous posters,  it's all oriented around goals/needs and how comfortable you are with your sterile technique.  I like one time use LI.  Make up enough to noc up  dozen jars from one plate and expand my best performing jars via g2g so I don't waste further jars on shitty, slow growing underperformers.  LC is great too, but I love LI.  I trust it.  Never been screwed over by it.  But yeah... 4cc is my target for either per 1qt. For my half gallons I don't exceed 10cc, etc, etc.


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"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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Offlinemycopiss
Stranger
Registered: 06/01/19
Posts: 352
Last seen: 1 year, 9 months
Re: Liquid Culture or Liquid Innoculant [Re: alaskappalachian]
    #26913753 - 09/02/20 01:14 PM (3 years, 4 months ago)

Cool. how long does it take on average for your jars to colonize with 4cc per 1qt?

Do you use the blender method with the oster blender blades that fit on the jar? Or just shake the LI Jar up after placing agar inside? Curious as to which you prefer.

Im definitely taking another look at my grain prep since I had slow colonization at 6cc, 15cc, and 24cc of LC per 4qt bag. I previously used agar and g2g with good results.
Thanks for the info.


Edited by mycopiss (09/02/20 02:11 PM)


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 10 hours
Trusted Cultivator
Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26913872 - 09/02/20 02:39 PM (3 years, 4 months ago)

I prefer blended li


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Invisiblealaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: Liquid Culture or Liquid Innoculant [Re: tryptkaloids]
    #26913962 - 09/02/20 03:29 PM (3 years, 4 months ago)

Quote:

mycopiss said:
Cool. how long does it take on average for your jars to colonize with 4cc per 1qt?

Do you use the blender method with the oster blender blades that fit on the jar? Or just shake the LI Jar up after placing agar inside? Curious as to which you prefer.

Im definitely taking another look at my grain prep since I had slow colonization at 6cc, 15cc, and 24cc of LC per 4qt bag. I previously used agar and g2g with good results.
Thanks for the info.



About 8-10 days for quarts depending on the culture.  I reeeeaallly should get an Oster and blend because I could knock a couple days off that...  (I just shake, although I have used a razor and wire attached to a lid in a jar to ghetto blend cornmeal plates and soft agar with great results).  I'm supporting 4 people on one income so I can only buy so many cult supplies per month but I'm snagging a goddamn Oster...) Once I get an idea of how the jars from my LI perform, go heavy on the G2G and it's like lightning.  Grain prep is the difference between night and day.  Also grain choice/inoculation points. 
Quote:

tryptkaloids said:
I prefer blended li




Blended LI is king of the ring.  Fuckin aye.:biker:


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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Offlinemycopiss
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Registered: 06/01/19
Posts: 352
Last seen: 1 year, 9 months
Re: Liquid Culture or Liquid Innoculant [Re: alaskappalachian]
    #26921486 - 09/06/20 04:11 PM (3 years, 4 months ago)

Damn. the LC I used looked ok, but I think the grain may have been bad. I tried with as little as 5 to 15cc per 4 quarts and still very very slow growth. I was having some contamination issues with other bags and tubs.

I will retry and experiment, some bags will get 4 to 5 cc per 1qt. then some will get 7, some will get 10cc per qt.

My bags which i put 60cc LI per 4qts are not showing much growth yet at day 10, just one little white speck. Too much liquid I suppose.


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 10 hours
Trusted Cultivator
Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26921491 - 09/06/20 04:13 PM (3 years, 4 months ago)

Or the grain was too dry.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinedirtybirdx
getting better
 User Gallery
Registered: 07/28/20
Posts: 250
Last seen: 2 years, 3 months
Re: Liquid Culture or Liquid Innoculant [Re: tryptkaloids]
    #26921666 - 09/06/20 06:00 PM (3 years, 4 months ago)

Hi pardon me for not reading the whole thread, it may have been mentioned already. 

I came to extol the righteousness of Munchauzen’s Blenderless Liquid Inoculate TEK.

Original and unorthodox, single use, no syringe, and best of all - full colonization (wbs) in 9 days.

Link to TEK in signature 👍


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Offlinemycopiss
Stranger
Registered: 06/01/19
Posts: 352
Last seen: 1 year, 9 months
Re: Liquid Culture or Liquid Innoculant [Re: dirtybirdx]
    #26922064 - 09/06/20 09:38 PM (3 years, 4 months ago)

Im a bit confused at this point.

Everybody says 10 to 15cc LI per quart is too much, but this blenderless Tek says to use 25-50ml per quart.


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Invisiblealaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26922096 - 09/06/20 09:54 PM (3 years, 4 months ago)

25-50ml of water per jar you inoculate with agar to make LI that you will use to make syringe with/pour; not per qt. I see how you might read it that way but that's what Munch is referring to in the tek.


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"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


Edited by alaskappalachian (09/07/20 02:22 AM)


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Offlinedirtybirdx
getting better
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Registered: 07/28/20
Posts: 250
Last seen: 2 years, 3 months
Re: Liquid Culture or Liquid Innoculant [Re: alaskappalachian]
    #26922298 - 09/07/20 01:35 AM (3 years, 4 months ago)

The only amount that is too much is the amount that creates a pool at the bottom of your grain jar or bag.  That’s going to be slightly different depending on your grain type and prep.

If it does pool at the bottom, hopefully it doesn’t stall.  If it doesn’t, when you shake at 25-30% the moisture at bottom should evenly distribute and they should recover and colonize well.  Otherwise it’ll just stall and never colonize.

Of course the constant game of lowering risk of contaminations includes the common knowledge that wetter equals higher % of contam.  I have a super low contam rate anyway, so no big deal.  But if you’re having tons of contam problems and using this tek you may want to aim for 10cc or less.


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Need help?




Edited by dirtybirdx (09/07/20 01:36 AM)


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Offlinemycopiss
Stranger
Registered: 06/01/19
Posts: 352
Last seen: 1 year, 9 months
Re: Liquid Culture or Liquid Innoculant [Re: dirtybirdx]
    #26928731 - 09/10/20 05:56 PM (3 years, 4 months ago)

I had no contam. but very minimal growth after more than 10 days now. very small spots of mycelium that have not grown much.
I also prepped the grain too dry. because I was worried about wet bags I had previously.

This was at 60cc to 4qt grain. There was no pooling but I believe my water to agar ratio for the LI was off. I used 1 agar tray for 500ml LI.
I now realize I should have used 3 trays for this amount after reading the blender LI Tek. He uses 1 Agar tray for every 180ml water.


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