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Offlinemycopiss
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Liquid Culture or Liquid Innoculant
    #26898800 - 08/25/20 01:24 PM (3 years, 5 months ago)

Is liquid culture better than liquid innoculant in any way? It seems it  can likely be stored longer. that is the only advantage I have found.

Which do you prefer and why?

I like LI because you do not have to wait for it to colonize. I will be trying my first run of it shortly.


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OfflineThe Mycologist
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26898808 - 08/25/20 01:27 PM (3 years, 5 months ago)

I thought they were synonymous.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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Offlinemycopiss
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Re: Liquid Culture or Liquid Innoculant [Re: The Mycologist]
    #26898816 - 08/25/20 01:29 PM (3 years, 5 months ago)

Liquid Innoculant is when you use a sterile blender to blend up the agar in water, then it is ready use immediately to inoculate.  No wait time for colonization.


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OfflineThe Mycologist
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26898821 - 08/25/20 01:34 PM (3 years, 5 months ago)

I see, well the con of that would be an additional vector of contam.


--------------------
"That you are here—that life exists, and identity;
That the powerful play goes on, and you will contribute a verse.”
― Walt Whitman, Leaves of Grass
:acidfire::tmckenna:


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OfflineGan
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Re: Liquid Culture or Liquid Innoculant [Re: The Mycologist] * 1
    #26898882 - 08/25/20 02:12 PM (3 years, 5 months ago)

It depends what you're using it for. LC is fantastic for expanding a culture substantially. Drop a small wedge and in just a bit you have enough LC to inoculate dozens of jars. So theoretically, you could take one clean culture (say a clone that you love) and expand it into gallons of LC and use it on hundreds of jars.

LI is great for if you have some clean plates and jars you want to inoculate but you dont want to go the a2g route. LI will allow that same plate to inoculate more jars (although not as many as LC) and they will colonize at a faster rate than if you just dropped an agar wedge in since the liquid will coat substantially more grains with the myc.

That's a general version of it imo. Though that doesn't cover everything. So it depends on the situation and what you want to do with a culture.

Also, LC = nutrient broth that supports culture growth. LI = sterile water that serves as a medium to spread mycelium.


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Offlinemycopiss
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Re: Liquid Culture or Liquid Innoculant [Re: Gan]
    #26898932 - 08/25/20 02:48 PM (3 years, 5 months ago)

is LC faster than LI at colonizing grains?


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InvisibleMunchauzen
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26898989 - 08/25/20 03:29 PM (3 years, 5 months ago)

Quote:

mycopiss said:
Liquid Innoculant is when you use a sterile blender to blend up the agar in water, then it is ready use immediately to inoculate.  No wait time for colonization.



no need for a blender :wink:

https://www.shroomery.org/forums/showflat.php/Number/22833314#22833314


LI usually made to be used immediately. LC is made to be stored.


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Offlinemycopiss
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Re: Liquid Culture or Liquid Innoculant [Re: Munchauzen]
    #26899140 - 08/25/20 05:11 PM (3 years, 5 months ago)

Nice I was just actually reading your tek yesterday.
Do you know how long LC Is good for?

When you say in your tek you transfer LI to receiving jars, you are speaking about grain jars correct? not additional LI jars?


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Invisiblehobbyguy
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss] * 1
    #26899143 - 08/25/20 05:17 PM (3 years, 5 months ago)

I just used a LC I had in a syringe for 1.5 years in a drawer. I forgot about it! Worked good  :smile:


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OfflineGan
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26899189 - 08/25/20 05:45 PM (3 years, 5 months ago)

Quote:

mycopiss said:

When you say in your tek you transfer LI to receiving jars, you are speaking about grain jars correct? not additional LI jars?




Yeah to grain jars.


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Offlinemycopiss
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Re: Liquid Culture or Liquid Innoculant [Re: Gan]
    #26912330 - 09/01/20 04:39 PM (3 years, 4 months ago)

How many ML of Liquid innoculant per Quart should be used?
I am trying 60cc per 4 quart bag. Did that four days ago. No growth yet.


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Invisiblemaxmush
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26912386 - 09/01/20 05:02 PM (3 years, 4 months ago)

Quote:

mycopiss said:
How many ML of Liquid innoculant per Quart should be used?
I am trying 60cc per 4 quart bag. Did that four days ago. No growth yet.




Is that a typo? 60cc/ml? or 6cc/ml?

60cc/ml is an insane amount IMO. I use only 3cc/ml LC.

I haven't used an LI before so maybe you need that much? Seems like a lot.


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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Offlinemycopiss
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Re: Liquid Culture or Liquid Innoculant [Re: maxmush]
    #26912402 - 09/01/20 05:11 PM (3 years, 4 months ago)

60 cc LI for 4 Quarts of grain.


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Offlinetryptkaloids
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26912521 - 09/01/20 06:17 PM (3 years, 4 months ago)

That's a lot.
I use 100 split between 8-10 quart jars


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Offlinemycopiss
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Re: Liquid Culture or Liquid Innoculant [Re: tryptkaloids]
    #26912536 - 09/01/20 06:26 PM (3 years, 4 months ago)

I just had an idea. How would using 2 Agar Trays per 1 quart Liquid innoculant work out? 

Theoretically it would colonize faster correct? But would it be too thick to suck up into an 18gauge syringe? I might give this a shot.

Also how long before you normally see colonization for LI? Using 60cc per 4qts oats I would have thought something would show by day 4.


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Offlinetryptkaloids
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26912542 - 09/01/20 06:31 PM (3 years, 4 months ago)

Why do you need a full quart of li?

I pour my li like g2g because i hate syringes.

But yes the more inoculation points the better. Just don't pour too much. Standing water is a no-no

You probably havent seen anytjing yet because you used so much liquid. .


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinemycopiss
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26912696 - 09/01/20 07:59 PM (3 years, 4 months ago)

You are doing 8 to 10 CC per 1qt correct? So do you think 40cc would be ok for 4 to 5 quart bags?

Should I have seen some type of growth by day 4? or does it usually take around a week?

In a post somewhere bod mentions you can use 10-15cc Liquid culture per Quart jar. According to what he said 60cc should be ok and should colonize faster.


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Invisiblemaxmush
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26912783 - 09/01/20 08:52 PM (3 years, 4 months ago)

I dunno like i said im not a practicing LI user lol but that just seems like too much liquid. It may not be colonizing because its contaminated? If you dont see anything after 7 days it could be toast. I think less is more in this case. Try 4qt bag with "only" 10cc instead of 60cc and see what happens. I think you'll be surprised.


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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InvisibleRoger Clemency
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Re: Liquid Culture or Liquid Innoculant [Re: maxmush]
    #26912796 - 09/01/20 08:58 PM (3 years, 4 months ago)

For me LI always shows up like 50% colonized on day 7, nothing until then so you could still be fine.

I’ve done 20ml per qt jar and they worked out fine. It was a little too much but I was just pouring away to be sure. Did you shake/mix the grain up real well after you poured the LI?


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Offlinemycopiss
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Re: Liquid Culture or Liquid Innoculant [Re: mycopiss]
    #26913320 - 09/02/20 07:50 AM (3 years, 4 months ago)

Yep I definitely mixed it up well. I previously tried Liquid culture before Liquid innoculant. I attempted 6cc, that was not nearly enough, very slow colonization. 15cc was also very slow.

24 CC LC per 4-5 qt bag is still not fully colonized and its been about 3 weeks.  I might try 30-40cc next. Im tired of messing with this shit I might just stick with grain to grain.


Edited by mycopiss (09/02/20 08:10 AM)


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