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Offlinemaniac1886
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Registered: 10/05/11
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Using WBS soak water for Grain LC?
    #26898489 - 08/25/20 10:43 AM (3 years, 5 months ago)

Im looking to change my LC technique to something a little more reliable.  In my readings, I have learned that you can use grain soak water in substitution for honey water and I like the idea of there being less bacterial contam potential due to less sugars.

I also read agar's writeup on grain lc.  Which is just inject sterile water into a grain jar, aspirate and remove.  However, it stands to reason that since there is little to no nutrition in said water, the viability of storage is probably less than a month.

I propose, instead of sterilizing plain water, could one sterilize grain soak water and perform the same steps, therefore adding nutrients to the medium as well as add longevity to the store-ability of said liquid culture?


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