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Offlinefightingcherries
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Sectoring in Cloned Culture
    #26897949 - 08/24/20 11:10 PM (3 years, 5 months ago)

I recently took a clone of a 5 gang nice cluster from a tub.  When I grew it out on agar, it sectored into about 6 clearly defined sectors. I choose the fastest and nicest looking one and transferred it to another dish and it did not sector when it fully colonized the dish.  I then recently did some reading on here, and saw that some people have said the clone can have a certain “synergy” with the other isolates in there and they may be necessary to keep all the traits I was looking for when I originally cloned the 5 gang.  Is there a way to clone and keep all of the sectors without them separating?  Only way I see is to throw the whole dish in a grain jar or bag.  I have not grown it out yet and now I am worried because I was really banking moving forward with this new culture.


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OfflineA.k.aM
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Re: Sectoring in Cloned Culture [Re: fightingcherries]
    #26898160 - 08/25/20 05:39 AM (3 years, 5 months ago)

The best strategy is to make plates from each sector and fruit them out to see which ones have what you’re looking for.

A couple times I had an awesome clone that I didn’t want to alter and so instead of dropping the whole plate I cut a circle around the whole plate halfway down and then did it again about a fifth of an inch away from the first cut.

That made it so I removed a thin circle of agar with all the mycelium sections on it. It was like a big ring.

I’ve also grown a plate out and then cut the very center where the original transfer wedge or tissue was and transferred it to another plate. That way it will regrow identical myc to the first plate and the first plate can be used to knock jars up.

Really this is mostly unnecessary and the way to avoid it is to narrow down a bunch of plates first then grow them so you can pick one and not worry about losing genetics.


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Edited by A.k.a (08/25/20 05:53 AM)


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Offlinefightingcherries
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Re: Sectoring in Cloned Culture [Re: A.k.a]
    #26899182 - 08/25/20 05:41 PM (3 years, 5 months ago)

I tried cutting a ring, like you said today.  With the second technique you mentioned, that seems like a great way to keep it going for a while to preserve the culture.  I wonder how many times you can do that?  Have you noticed when you did, if ever, grow out one of the sectors, that it was significantly different from the original?  Is it possible, it can be a total waste?  Or is it only slightly different?


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OfflineA.k.aM
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Re: Sectoring in Cloned Culture [Re: fightingcherries]
    #26899362 - 08/25/20 07:20 PM (3 years, 5 months ago)

I don’t have a ton of experience doing a lot of work on a clone, I usually just fruit it once and if it’s good make a plate of it and move on because I’ve got a ton of different varieties going.

I have noticed a definite difference in early transfers though. I had a PE clone where I grew a tub from the first plate with the tissue on it and then I think from t2.  The first one right from the tissue had way more variety, the later plate was way more uniform with a dense pinset and mostly identical mushrooms.

The t2 tub was so uniform I can’t imagine more transfers making a big difference.


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Offlinetryptkaloids
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Re: Sectoring in Cloned Culture [Re: A.k.a]
    #26899455 - 08/25/20 08:24 PM (3 years, 5 months ago)

I wouldn't worry too much about getting nothing. Not what you expected sure, but its not as likely as the internet makes it seem to get "non fruiting" genetics. just don't isolate it too much you should be fine


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Offlinefightingcherries
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Re: Sectoring in Cloned Culture [Re: tryptkaloids]
    #26899603 - 08/25/20 10:11 PM (3 years, 5 months ago)

Thank you man, easing my mind for sure.  This had me anxious as hell.


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OfflineA.k.aM
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Re: Sectoring in Cloned Culture [Re: fightingcherries]
    #26899948 - 08/26/20 05:49 AM (3 years, 5 months ago)

Yeah i actually have read it’s not possible to get a non fruiting culture from a clone. I’ve definitely not done enough work on them to know for sure myself though.

Just keep in mind the more isolating you do the more consistent the grows will be, but that goes for shitty genes too.


I’ve always though of it like say there’s a bag full of money which represents genetics and you can either grab a handful and keep whatever the average amount is which would be like an ms grow, or you can pick one bill and keep it which would be like an isolate. If you happen to grab 100 then awesome but you could also get a 1.


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