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OfflineAwake_and_Away
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Registered: 02/08/18
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What went wrong?
    #26897619 - 08/24/20 06:14 PM (3 years, 5 months ago)

Hi all, just thought I would share a bit about my first attempt at cultivating mushrooms a while ago, and see if any of you might like to offer some advice should I make another attempt at it.



5 days post-inoculation I saw some condensation in one of the twelve jars near one inoculation site, which turned out to be the first sign of a trich contam. After 7 days the trich contam was obvious and one more jar had begun to show condensation, but not localised to the inoculation site (condensation covered a section of about 1/4 of the jar's walls). After 7 days NO jars showed any visible mycelium.

I know people on this forum freak out when anyone mentions opening contaminated jars indoors, but I wanted to see what was going on inside the contaminated jar before I binned it... for science. I opened it up inside a trash bag in my kitchen - where moldy jars of tomato sauce, jams etc. have been unassumingly opened before, so mold in the area was nothing new. Turned out the trich was localised to one inoculation port, just below the dry verm layer, in about a 15x15x5 millimetre area. I remember what struck me was how dry the cake felt. It had the texture of a soft, crumbly pencil eraser. Hardly felt moist at all - is that normal? How sensitive is the 2-1-1 ratio?

The trich growth seemed to have stalled in the days between initially appearing and the jar being opened - perhaps the cake was too dry and its growth was limited by the volume of the spore solution injected at that port?

Anyway, I tossed the cakes... my conclusion at the time was that I had made some dud cakes, as although I didn't crumble and inspect every inch of every cake, none except the two aforementioned ones showed any signs of life, and none showed any macroscopic evidence of mushroom mycelium or hyphae at all.

But perhaps the spores could have been hydrated in the syringe for an extended period of time prior to inoculation?

Or perhaps I might have simply been too impatient? Could the jars really have shown zero signs of life after 8 days, yet contain spores that would still conceivably have germinated had I waited longer?


Details about the TEK:

I had some spores of a commonly grown species obtained from a supposedly reputable spore exchange based in Spain and more or less followed Hippie3's tek when making the BRF + Verm Jars - wide mouth half pint, 2-1-1 verm/brf/water ratio (volumetric and by eye - no measuring jug or scale - mistake #1?), sterilised in crock pot (electric model, rated up to 10 PSI I think) on steam setting for 45 minutes at high pressure, removed foil under SAB and covered inoculation ports with micropore tape (maybe not tightly applied - mistake #2?).

SAB setup was just an inverted plastic container with two holes on a counter, and before use the inside was misted through the holes.

The spores themselves were dated 1-2 years old at the time, but when I got them it was winter and they may have sat in an outdoor mailbox at subzero temperatures for some time - could I have been doomed to fail from the start with dead spores?

I used Stro's Sterile Syringe/Spore Syringe TEKs to make the syringes - should I have let the spores hydrate inside the syringes for and extended period? Under pressure?

If I still had spores from the same shipment that were dated even older (e.g. 5-6 years old), but that also could have been subjected to subzero temperatures, would attempting a grow with them be a lost cause? Or would it be worth a shot to try something with them until perhaps obtaining fresher prints? (There needs to be a CA based spore trader called Fresh Prints of Bel Air, just throwing that one out there)

Does anything I wrote indicate a flaw in my methodology that caused my grow to completely fail to produce any results at all? Or do you think my spores were the issue?



I'm sure I haven't given enough information for anyone to be able to draw any definite conclusions, so please ask follow ups if you are willing to assist me and need more info!

Thanks to this great community,

-Awake_and_Away


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