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Need to get my agar right! Please help.
    #26897465 - 08/24/20 04:40 PM (3 years, 5 months ago)

I first got into this hobby with MSS to brown rice.
Then I tried agar and wasn't 100% if I was doing it right, had some funny looking plates.

I didn't bother for a while as my plates never looked as good as the ones online so I stashed them all away.

I recently found the plates, many of them survived. Clones to transfers to 2nd transfers survived. I then gave it another go to take one of 2nd transfer and do a 3rd but got green nasties. Looks easily recoverable if peroxide will work. How effective is 3% solution? Pour onto plate or cotton bud?

Cookie rack or no rack? Towel or no towel? Bleach water or warm soap to settle air? I used a mini kitchen gas jet with the blue flames to sterilise my scalpel but not sure if it got hot enough. It got red but would fade away quick.

I just want to get this shit right. I've ordered dextrose too to try LC. Any tips or sterile technique tips would be great. Arm hole cover recommendations? Any order of spraying and not spraying? Thank you


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Re: Need to get my agar right! Please help. [Re: Hide]
    #26897485 - 08/24/20 04:53 PM (3 years, 5 months ago)

You don't fix contamination you carefully transfer away from it as early as possible or best case just toss those dishes and work from non-contaminated ones.

don't try to kill contamination on a petri dish. It won't work and you'll have a bad time.


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