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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlinemycopiss
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Registered: 06/01/19
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Liquid Culture Questions
    #26897010 - 08/24/20 11:52 AM (3 years, 5 months ago)

I am attempting liquid culture for the first time and have several questions, some of these questions I can find by searching sure, but I seem to find mixed responses.

1. Are rubber gaskets needed for the white plastic jar lids or the Ball Leak Proof Lids?  I turned it upside down with water inside, no leaking, is the gasket necessary?

2. Are syringe filters the best for GE? does it matter if its PTFE or PVDF Filter?

3. How many ML per 1 Quart of Grains? I am using 5 Qt Grain bags. I tried 20ML , and this is not nearly enough as colonization is very minimal after 1 week.
 
4. How long is a LC jar good for if refrigerated? I was told you need to use LC ASAP, but other info I have found contradicts what I was told

5.  When I inoculate, should I shake or stir at that time?


6.  Are glass syringes ok? I would prefer this over using many syringes and creating more trash. I am aware that pouring into the bag may be easier but I want to find the right amount of LC, and be accurately measured for every bag.  I may grab 50ML Glass syringes.

7. What kind of Impulse sealer to use for 2mil Bags? 2mm seal width seems to not be working great.

Sorry for so many questions haha.


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