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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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Offlinemycopiss
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Did I kill the mycelium?
    #26896902 - 08/24/20 11:14 AM (3 years, 5 months ago)

I made some liquid innoculant last night and forgot to let the jar cool off before I dropped the agar inside.  I realized my mistake immediately and put the jar into the fridge.

The Jar  was 110F when I inoculated, is this hot enough to kill the mycelium? is my liquid inocculant ruined?


Edited by mycopiss (08/24/20 11:26 AM)


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OfflineMycochopper
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Re: Did I kill the mycelium? [Re: mycopiss]
    #26896946 - 08/24/20 11:28 AM (3 years, 5 months ago)

I believe that TMC says that thermal death occurs at 106 degrees.


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InvisibleGoatrider
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Re: Did I kill the mycelium? [Re: mycopiss]
    #26896951 - 08/24/20 11:29 AM (3 years, 5 months ago)

I`m afraid it`s above death temp, but just have a look what happens.

Edit: faster fingers by Mycochopper :wink:


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Offlinemycopiss
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Re: Did I kill the mycelium? [Re: Goatrider]
    #26896972 - 08/24/20 11:36 AM (3 years, 5 months ago)

I suppose we Shall test it on an agar plate. I Will let yall know what happens.


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InvisibleGoatrider
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Re: Did I kill the mycelium? [Re: mycopiss]
    #26896975 - 08/24/20 11:37 AM (3 years, 5 months ago)

Good luck :thumbup:


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Offlinemycopiss
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Re: Did I kill the mycelium? [Re: Goatrider]
    #26896980 - 08/24/20 11:38 AM (3 years, 5 months ago)

Im going to make another because I know its likely ruined, but shit why not test it.  I like LI as its faster than waiting for LC to colonize.

What are your preferences all got preference Liquid innocculant or Liquid culture? reasons? Pros, Cons?


Edited by mycopiss (08/24/20 04:54 PM)


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OfflineMycochopper
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Re: Did I kill the mycelium? [Re: mycopiss]
    #26897159 - 08/24/20 01:31 PM (3 years, 5 months ago)

Found this old thread that may give you some more info on Temps.  Apparently they are more durable than we think.

https://www.shroomery.org/forums/showflat.php/Number/6209334#6209334


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