|
dr s
Stranger

Registered: 07/04/20
Posts: 42
Last seen: 2 years, 9 months
|
Tubs colonizing in different ways
#26895687 - 08/23/20 04:41 PM (3 years, 5 months ago) |
|
|
I am doing 2 tubs right now (first grow) and they are colonizing differently - tub 1 is MS>oats>tub just for fun, and tub 2 is MS>agar>oats>tub.
They are different varieties, both cubes. Tub 1 variety was slow and tomentose on agar, Tub 2 variety was fast and rhizo on agar, and the tubs seem to reflect this. Tub 1 is 3qt spawn/6qt coir - Tub 2 is 3qt spawn/9qt coir. I used water amounts noted in teks and both appeared to be at correct field capacity
Tub 1 is at 5 days - while the top has continued visibly growing each day, none of the sides look very filled in
Tub 2 is at only 2 days - and while the top still looks very early, the sides actually look more filled in than Tub 1 does at 5 days
Pics are labeled - is something up with Tub 1 - or is it just a different kind of growth??
|
Spore211215
SporeGuy

Registered: 06/06/16
Posts: 108
Last seen: 1 year, 8 months
|
Re: Tubs colonizing in different ways [Re: dr s]
#26895768 - 08/23/20 05:47 PM (3 years, 5 months ago) |
|
|
You explained in all in the first line. MS is the reason why, if you isolated down on agar a bit both batches would likely see similar growth patterns. Going straight MS to grain led to this
|
jcm4620
Stranger


Registered: 05/26/19
Posts: 6,700
Last seen: 1 year, 11 months
|
Re: Tubs colonizing in different ways [Re: Spore211215]
#26895773 - 08/23/20 05:53 PM (3 years, 5 months ago) |
|
|
they are both a ms inoculation and the reason why is strictly a genetical issue if the substrates are identical and there is no signs of contam in either tub.
just cuz u use agar doesnt make it not a ms inoculation. unless its a clone or other actual known set of genetics its a ms inouclation. just cuz you transfered a sector from a spore inoculated plate to a new plate doesnt make that transfer any less of a ms inoculation than using the initial spore plate or shooting it up with a syringe or any other method from which the initial culture used is originated from when spores were used
Edited by jcm4620 (08/23/20 06:20 PM)
|
dr s
Stranger


Registered: 07/04/20
Posts: 42
Last seen: 2 years, 9 months
|
Re: Tubs colonizing in different ways [Re: jcm4620]
#26895782 - 08/23/20 06:01 PM (3 years, 5 months ago) |
|
|
Just to clarify - Tub 1 is MS > grain > tub Tub 2 is MS > cleaned up on agar > grain > tub
But you guys are saying the different growth in Tub 1 is kind of random MS growth? Just thought it was odd that the top was colonizing but the sides did not appear to be - or maybe sides are colonizing and it's just harder to tell. Just a big difference visually between the two so figured I'd ask
|
smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 12 days, 7 hours
|
Re: Tubs colonizing in different ways [Re: dr s]
#26895786 - 08/23/20 06:08 PM (3 years, 5 months ago) |
|
|
You cant really say that this ms or that ms does this or that, because genetic variety is already so big that it could swing low or high to begin with
-------------------- Willpower is the one true virtue
  
|
Tripglass420
Old Head back from hiatus


Registered: 01/27/20
Posts: 200
Loc: Southeast
Last seen: 2 years, 9 months
|
|
I mean you did use 3-6 in one and 3-9 (spawn to sub ratio)
So that would be an obvious reason for different looks comparing each tub.
And then yes using ms to agar is simply just germinating your spores on the agar versus the grain which helps with contamination but it's still growth from spores and also having less variety in each tub .
Maybe the tormentose type growth was kept out of tub 2 because of the agar clean up.
they look great either way just be patient considering the ratio used was greater than lots of tubs I've seen. Happy shroomin!!
|
dr s
Stranger


Registered: 07/04/20
Posts: 42
Last seen: 2 years, 9 months
|
Re: Tubs colonizing in different ways [Re: Tripglass420]
#26897744 - 08/24/20 07:33 PM (3 years, 5 months ago) |
|
|
Thanks for the responses - on the spawn yes it's just that I used 3/6 on the one that's going slower (or visually slower anyway) than the one I did 3/9 and that one is only 2 days in
Part of why I asked too though is I've never seen a fully colonized tub in person. Tub 2 it's pretty obvious to me watching it go, but for tub 1 I'm unsure if the sides etc are colonized as they have very little visual changes - I guess I should just go off the look of the top? I'm getting a dry corner so was going to mist soon, just didn't want to mist too early if it's not fully colonized - thoughts? Pics:
full tub today, 6 days in

spot that looks good moisture wise (I think)

this corner has looked dry for several days
|
smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 12 days, 7 hours
|
Re: Tubs colonizing in different ways [Re: dr s]
#26898853 - 08/25/20 01:54 PM (3 years, 5 months ago) |
|
|
the one you call dry is definitely dry, and wants to be more moist with beads of moisture on the surface
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 181 topic views. 27 members, 176 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|