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OfflinePhishgrrl
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another syringe question-pc'ing w/alcohol & water
    #2689475 - 05/17/04 01:21 AM (14 years, 7 days ago)

K- so a friend tried the karo tek and her syringe with the myc got contamned. So she rinsed the syringe out (since that was her only one and she needs to reuse it with the jar she thankfully innoculated with the karo tek) with hot water and alcohol, maybe 10-20% alcohol, lots of times, then filled the syringe about 1/2 full of the alcohol and h20 solution, since she couldn't fit the full syringe in the pc and pc'd it for about 25 min. She is going to squirt out the solution before she reuses the syringe but is worried that perhaps she made a mistake in not using just water- will the teeny remainder of alcohol in the syringe kill the mycelium she is going to suck into it?  Should she re-sterilize the syringe with just water rather than risking killing the myc? Sorry if this is confusing. :syringe:


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OfflineLateForTheFuture
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Re: another syringe question-pc'ing w/alcohol & water [Re: Phishgrrl]
    #2689523 - 05/17/04 01:29 AM (14 years, 7 days ago)

I reccomend, if you're worried about some sort of residue, to just boil some water and suck it through the syringe repeatedly. Alcohol usually dissipates very well if its in open air, and I used it all the time in my procedures and never had a problem.


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Invisiblemuse_sick
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Re: another syringe question-pc'ing w/alcohol & water [Re: Phishgrrl]
    #2689534 - 05/17/04 01:31 AM (14 years, 7 days ago)

how did you know yr syringe was contamed?


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OfflinePhishgrrl
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Re: another syringe question-pc'ing w/alcohol & water [Re: muse_sick]
    #2689545 - 05/17/04 01:33 AM (14 years, 7 days ago)

because her syringe had a black line appear around it after about a week of incubation. Looked like some kind of mold or mildew. I think this may have happened because there was a bit of air in the syringe (?) Could it have been something other than contamination?


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Invisiblemuse_sick
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Re: another syringe question-pc'ing w/alcohol & water [Re: Phishgrrl]
    #2689598 - 05/17/04 01:45 AM (14 years, 7 days ago)

no a black line def sounds like a contam
i usually suck boiled water up into my syringes between uses
and then between injections i clean with a flame (from a crack lighter)
and h2o2


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OfflinePhishgrrl
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Re: another syringe question-pc'ing w/alcohol & water [Re: muse_sick]
    #2689607 - 05/17/04 01:47 AM (14 years, 7 days ago)

Cool, thanks :smile:


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