Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Cultivation Supplies

Jump to first unread post Pages: 1 | 2 | 3 | 4 | 5 | Next >  [ show all ]
OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 9 months
How my Liquid Culture looks like? How long it takes bevore using?
    #26893744 - 08/22/20 03:26 PM (3 years, 5 months ago)

Good evening :smile: this is my first Liquid Culture make it 5 days ago and i can see the Mycelium Growing litte White Spots Cooool... How is the chance everything goes right this time? I take honey 4% and osmosis water sterelisit let it Cool down and Injecting Spores in it 1cc per Liquid. I hope i do it correct and the liquid is fine and clean.

How long it needs to Grow bevore i can using it? How much i need for that stuff also 1cc with mycelium ore litte bit more? Thank you





Extras: Filter Print Post Top
Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen] * 1
    #26893829 - 08/22/20 04:18 PM (3 years, 5 months ago)

I think you're gonna get the same  answer from most people here: don't go straight MS syringe to LC. Instead use agar first.

This is for a variety of reasons but 2 of the most important are:
1. to clean the spores from contamination
2. to sector/select the best myc growth

By going straight to LC, the chances of you wasting your time with contamination is very HIGH.


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


Extras: Filter Print Post Top
Offlinesendmehummus
AT Hiker
Male User Gallery

Registered: 08/03/20
Posts: 383
Loc: USA Flag
Last seen: 2 years, 3 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
    #26893841 - 08/22/20 04:25 PM (3 years, 5 months ago)

What he said: go agar to LC. Mt first ever LC batch has also been at it for a little over a week now. Solution is cloudy and can see nice whispy bits floating around. Im also curious when it is a prime time to transfer it. Im not going straight to grain jars instead Im just making a bunch of LC syringes. Was gonna give it one more week but if a pro could chime in with any visual queues to look for before transferring that'd be great!


--------------------
Be patient and keep it simple.


Extras: Filter Print Post Top
OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 9 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: sendmehummus]
    #26894725 - 08/23/20 03:12 AM (3 years, 5 months ago)

I have my Syringe empty so hopefully it was a success :frown: without Aggar tek. I see litte White Mycelium Growing so good sign?

Can i using Agar Plates now with the LC and after that transfer to Rye Jars?


Here you see a better picture how the LC looks




Extras: Filter Print Post Top
OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 9 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
    #26894726 - 08/23/20 03:14 AM (3 years, 5 months ago)

Quote:

maxmush said:
I think you're gonna get the same  answer from most people here: don't go straight MS syringe to LC. Instead use agar first.

This is for a variety of reasons but 2 of the most important are:
1. to clean the spores from contamination
2. to sector/select the best myc growth

By going straight to LC, the chances of you wasting your time with contamination is very HIGH.





Are the plates the only think i need? I found a pack on Amazon.

https://www.amazon.de/Servoplate-C3-10413-N%C3%A4hrb%C3%B6den-Sabouraud/dp/B00CS2IQOK/ref=mp_s_a_1_1?dchild=1&keywords=agar+platten&qid=1598173989&sprefix=Agar+p&sr=8-1


Extras: Filter Print Post Top
Offlinesendmehummus
AT Hiker
Male User Gallery

Registered: 08/03/20
Posts: 383
Loc: USA Flag
Last seen: 2 years, 3 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26894728 - 08/23/20 03:19 AM (3 years, 5 months ago)

A roll of parafilm is also super handy to have to seal your plates 👍 On amazon as well. Disposable scalpels are also really useful for agar work, but Im sure you could make other tools work that can easily sterilized. I just prefer scalpels.
And yes you can use your new LC on plates, in fact most ppl would tell you its a vital step of any homemade LC to test it on a plate before using it to inoculate jars. It will give you a clear picture of how clean your LC is. 😁🤞


--------------------
Be patient and keep it simple.


Edited by sendmehummus (08/23/20 03:22 AM)


Extras: Filter Print Post Top
Offlinedothedew69
Relearning
 User Gallery


Registered: 08/27/08
Posts: 624
Last seen: 3 years, 3 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: sendmehummus]
    #26894776 - 08/23/20 04:54 AM (3 years, 5 months ago)

That’s exactly what I was intending to do, made up 3 lme lc’s and just got all my agar supplies in today. Now I’m just deciding on which agar recipe to use. Already have lme and dextrose, or follow bod and use the water from my grains, what to do. Will use sab, but I’m trying to figure out a blower for my hepa filter, much rather do agar in a flow hood.


Extras: Filter Print Post Top
OfflineA.k.aM
Stranger
 User Gallery


Registered: 10/27/19
Posts: 16,816
Loc: Gaming the system
Last seen: 3 hours, 4 minutes
Trusted Cultivator
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: dothedew69]
    #26894896 - 08/23/20 07:41 AM (3 years, 5 months ago)

Well you’ll need plates, a media bottle, agar and nutrients. You can use almost anything for nutes though. And a torch.


The problem with lc is that sugary liquid is the perfect environment for bacteria and mold to grow in. Your syringe will almost definitely have them inside. If even one bacteria gets into the lc it will multiply rapidly because it’s a perfect environment. That’s why spores don’t usually work for lc.


When I first started I made lc from syringe too, but I was lucky and the place I got my syringes made them extremely clean.

This is how mine looked like a week in.


You can actually see the ungerminated spores stuck in it.


--------------------
LAGM2020


Edited by A.k.a (08/23/20 07:43 AM)


Extras: Filter Print Post Top
OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 9 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: sendmehummus]
    #26895016 - 08/23/20 08:58 AM (3 years, 5 months ago)

Quote:

sendmehummus said:
A roll of parafilm is also super handy to have to seal your plates 👍 On amazon as well. Disposable scalpels are also really useful for agar work, but Im sure you could make other tools work that can easily sterilized. I just prefer scalpels.
And yes you can use your new LC on plates, in fact most ppl would tell you its a vital step of any homemade LC to test it on a plate before using it to inoculate jars. It will give you a clear picture of how clean your LC is. 😁🤞





I don't have a pressure cooker yet also a mistake i make not buying it bevore i do anything... This one here ok https://www.amazon.de/gp/aw/d/B00HX3W2G2/ref=ox_sc_act_image_2?smid=A2RU6HKNJFBS8D&psc=1

OK, than i will give the Dishes a try but i need a air box to? Normally i do the work in the bath but i know it's not optimal!

Can i use a normal syringe to suck out the Mycelium ore did i need a special needle?


Extras: Filter Print Post Top
OfflineJohn in WI
Neo-Luddite
 User Gallery

Registered: 12/18/13
Posts: 358
Loc: On a hippy trail head ful...
Last seen: 1 day, 16 hours
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26895022 - 08/23/20 09:03 AM (3 years, 5 months ago)

Their was a TEK--and PLEASE if you remember what it was, let me know!--where one would inoculate spores straight to LC, let them germinate, then hit the LC with hydrogen peroxide to kill bacteria and any bacterial spores.  In theory, the mycellium should not be killed by the H2O2, but any spores (bacterial or fungal) should be chewed up.  I seem to remember using a very low concentration of sugar (1%?), letting it grow out, then blasting it with peroxide.

Does anyone remember the process for doing that?  I'm asking, because I was trying to figure out how to make a mixed LC from several impressive spore prints.  I've tried numerous times to go from spores straight to LC, and always ended up with cloudy, clearly unusable solutions.  Bacteria water with bits of mycellium in it.


Edited by John in WI (08/23/20 09:08 AM)


Extras: Filter Print Post Top
OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 9 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: A.k.a]
    #26895039 - 08/23/20 09:18 AM (3 years, 5 months ago)

Quote:

A.k.a said:
Well you’ll need plates, a media bottle, agar and nutrients. You can use almost anything for nutes though. And a torch.


The problem with lc is that sugary liquid is the perfect environment for bacteria and mold to grow in. Your syringe will almost definitely have them inside. If even one bacteria gets into the lc it will multiply rapidly because it’s a perfect environment. That’s why spores don’t usually work for lc.


When I first started I made lc from syringe too, but I was lucky and the place I got my syringes made them extremely clean.

This is how mine looked like a week in.


You can actually see the ungerminated spores stuck in it.






I blown away if my project fail again :frown: bought the 2 syringe the first was a fail but my stupidity know i make everything new but again without agar but the Syringe is maybe clean enough so my Liquid looks OK ore isn't? Bought it litte longer in the jar.


Extras: Filter Print Post Top
Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26895199 - 08/23/20 11:06 AM (3 years, 5 months ago)

Quote:

Fietchen said:
Quote:

sendmehummus said:
A roll of parafilm is also super handy to have to seal your plates 👍 On amazon as well. Disposable scalpels are also really useful for agar work, but Im sure you could make other tools work that can easily sterilized. I just prefer scalpels.
And yes you can use your new LC on plates, in fact most ppl would tell you its a vital step of any homemade LC to test it on a plate before using it to inoculate jars. It will give you a clear picture of how clean your LC is. 😁🤞





I don't have a pressure cooker yet also a mistake i make not buying it bevore i do anything... This one here ok https://www.amazon.de/gp/aw/d/B00HX3W2G2/ref=ox_sc_act_image_2?smid=A2RU6HKNJFBS8D&psc=1

OK, than i will give the Dishes a try but i need a air box to? Normally i do the work in the bath but i know it's not optimal!

Can i use a normal syringe to suck out the Mycelium ore did i need a special needle?




You should get a regular stove top pressure cooker that can go to at least 15psi. The electric ones dont work as well, but I have used an Instant Pot successfully and PC for 45min. Use a simple 4% karo (corn syrup) solution with distilled water. This is for the LC (which) you already made). I *think* using it for agar should work as well (although not ideal).

Another option is to but pre-poured agar. I am not sure if they ship to Germany (i am assuming this is where you are), but you can check with each supplier as some do send internationally.

Parafilm is best to keep them contam free and to help keep moisture content, but you can use micropore tape (3m) or any first aid paper tape as well. Use this in 2 layers. I have also used regular black electrical tape.

You will need a tool to cut the agar once it has colonized and re-introduced to the 4% LC solution. You can use an excato knife or a scalpel (amazon). You will first clean the entire tool with iso 70% let dry and then flame sterilize with a touch or even a simple lighter. This tool will also be used to cut and transfer the "clean" myc away from contams to a new agar plate. You will likely have to do this 2-3x to clean it.

Yes you will need a SAB for sure for agar work. Dont try this open air or you will be disappointed.

Steps:
1. make agar plates
2. swirl your LC mixture well and draw up the smallest amount of LC possible (1/10cc .1ml)
3. carefully open your agar plate avoiding putting your hand over it and squirt the smallest amount of LC on the agar (1-2 drops max)
3. close and seal
4. let sit at room temp (18-20c) and watch it grow out
5. when you see contams and/or healthy myc, cut the myc carefully (small piece furthest away from any contam and transfer to a new clean agar dish.
6. repeat until clean
7. use clean agar to inoculate your new freshly made LC solution of choice

Viola! clean LC ready to use in about 10-12 days


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


Extras: Filter Print Post Top
OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 9 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
    #26895286 - 08/23/20 11:52 AM (3 years, 5 months ago)

Quote:

maxmush said:
Quote:

Fietchen said:
Quote:

sendmehummus said:
A roll of parafilm is also super handy to have to seal your plates 👍 On amazon as well. Disposable scalpels are also really useful for agar work, but Im sure you could make other tools work that can easily sterilized. I just prefer scalpels.
And yes you can use your new LC on plates, in fact most ppl would tell you its a vital step of any homemade LC to test it on a plate before using it to inoculate jars. It will give you a clear picture of how clean your LC is. 😁🤞






I don't have a pressure cooker yet also a mistake i make not buying it bevore i do anything... This one here ok https://www.amazon.de/gp/aw/d/B00HX3W2G2/ref=ox_sc_act_image_2?smid=A2RU6HKNJFBS8D&psc=1

OK, than i will give the Dishes a try but i need a air box to? Normally i do the work in the bath but i know it's not optimal!

Can i use a normal syringe to suck out the Mycelium ore did i need a special needle?




You should get a regular stove top pressure cooker that can go to at least 15psi. The electric ones dont work as well, but I have used an Instant Pot successfully and PC for 45min. Use a simple 4% karo (corn syrup) solution with distilled water. This is for the LC (which) you already made). I *think* using it for agar should work as well (although not ideal).

Another option is to but pre-poured agar. I am not sure if they ship to Germany (i am assuming this is where you are), but you can check with each supplier as some do send internationally.

Parafilm is best to keep them contam free and to help keep moisture content, but you can use micropore tape (3m) or any first aid paper tape as well. Use this in 2 layers. I have also used regular black electrical tape.

You will need a tool to cut the agar once it has colonized and re-introduced to the 4% LC solution. You can use an excato knife or a scalpel (amazon). You will first clean the entire tool with iso 70% let dry and then flame sterilize with a touch or even a simple lighter. This tool will also be used to cut and transfer the "clean" myc away from contams to a new agar plate. You will likely have to do this 2-3x to clean it.

Yes you will need a SAB for sure for agar work. Dont try this open air or you will be disappointed.

Steps:
1. make agar plates
2. swirl your LC mixture well and draw up the smallest amount of LC possible (1/10cc .1ml)
3. carefully open your agar plate avoiding putting your hand over it and squirt the smallest amount of LC on the agar (1-2 drops max)
3. close and seal
4. let sit at room temp (18-20c) and watch it grow out
5. when you see contams and/or healthy myc, cut the myc carefully (small piece furthest away from any contam and transfer to a new clean agar dish.
6. repeat until clean
7. use clean agar to inoculate your new freshly made LC solution of choice

Viola! clean LC ready to use in about 10-12 days






Cool Man... Thx a lot!

Look at my Baby Mycelium looking sweet what you think?

You say 1-2 drops of LC this mean 1-2 drops of mycelium drops? It's hard know to suck in the litte white spots so how much i need to wait bevore i can incect the lq to agar dishes? I will buy plates tomorrow and built a sterile box and working there.

I will keep Updating here but everyone else is welcome here too with there stuff.


Extras: Filter Print Post Top
OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 9 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: John in WI]
    #26895292 - 08/23/20 11:53 AM (3 years, 5 months ago)

Quote:

John in WI said:
Their was a TEK--and PLEASE if you remember what it was, let me know!--where one would inoculate spores straight to LC, let them germinate, then hit the LC with hydrogen peroxide to kill bacteria and any bacterial spores.  In theory, the mycellium should not be killed by the H2O2, but any spores (bacterial or fungal) should be chewed up.  I seem to remember using a very low concentration of sugar (1%?), letting it grow out, then blasting it with peroxide.

Does anyone remember the process for doing that?  I'm asking, because I was trying to figure out how to make a mixed LC from several impressive spore prints.  I've tried numerous times to go from spores straight to LC, and always ended up with cloudy, clearly unusable solutions.  Bacteria water with bits of mycellium in it.





I need that TEK lol. Search it please :smile: i can buy here Peroxide with 3%


Extras: Filter Print Post Top
OfflineJohn in WI
Neo-Luddite
 User Gallery

Registered: 12/18/13
Posts: 358
Loc: On a hippy trail head ful...
Last seen: 1 day, 16 hours
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26895457 - 08/23/20 01:53 PM (3 years, 5 months ago)

I wasn't on this forum for around 8 years, and in that time  I forgot what to search for!  I'm thinking of messing around with it myself. To make an LC with spores directly to solution, then trying to kill bacteria/fungal spores with H2O2 afterwards.  I've never had success using spores or spore solution directly to LC, regardless of how long I pressure cooked the solution.  The problem (for me) is not infected LC solution--it's infected spores. Every spore print or solution are contaminated.  They grow in dirty environments.  Some do have some success going spores to grain, but that is very dicey too.

I'm following this thread and will try and find the peroxide tek.

It might be possible to take your mycellium solution, even if it's contaminated, and put it on agar and clean it up that way? Or perhaps you could go with your mycellium solution to PF cakes, and clean it up like that.  The brown rice flour on vermiculite is supposed to give bacteria a very rough time.  Something to think about.


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,647
Loc: Exact Center
Last seen: 18 minutes, 40 seconds
Trusted Cultivator
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: John in WI]
    #26895471 - 08/23/20 02:02 PM (3 years, 5 months ago)

Quote:

John in WI said:
I wasn't on this forum for around 8 years, and in that time  I forgot what to search for!  I'm thinking of messing around with it myself. To make an LC with spores directly to solution, then trying to kill bacteria/fungal spores with H2O2 afterwards.  I've never had success using spores or spore solution directly to LC, regardless of how long I pressure cooked the solution.  The problem (for me) is not infected LC solution--it's infected spores. Every spore print or solution are contaminated.  They grow in dirty environments.  Some do have some success going spores to grain, but that is very dicey too.

I'm following this thread and will try and find the peroxide tek.

It might be possible to take your mycellium solution, even if it's contaminated, and put it on agar and clean it up that way? Or perhaps you could go with your mycellium solution to PF cakes, and clean it up like that.  The brown rice flour on vermiculite is supposed to give bacteria a very rough time.  Something to think about.



Using h2o2 for mycology is largely outdated. Use clean innoculant via agar to inoculate your lc... Even then you want to test it before using it.

@OP
Your lc doesn't look horrible. I would give it more time. Like a week or 2. Then take it to grain and put a couple drops on a couple plates to keep the culture


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: John in WI]
    #26895477 - 08/23/20 02:05 PM (3 years, 5 months ago)

Just a caveat: it is very frowned upon here on Shroomery to use h202 (peroxide) for anything mycology related. I personally am still interested in peroxide teks for back up purposes only and not for primary use. 

OP and John in WI, the use of agar to clean spores and/or LCs in now standard procedure. I recommend NOT proceeding without this. It will actually save you time and definitely heartache.

OP your "LC" is not close to ready yet. You should wait until you see clouds or cotton ball like growths before using. Once at this stage swirl the LC very briskly to break up the myc into tiny pieces and then suck it up with a 14g-20g needle. You can get away with a 22g if your myc is broken up very well. Also next time put a glass marble or piece of broken glass before PCing to help agitate the solution. Better yet, put a magnetic stir bar in and use a stir plate. You can by these really cheap on Amazon.

Best of luck!


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


Extras: Filter Print Post Top
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,647
Loc: Exact Center
Last seen: 18 minutes, 40 seconds
Trusted Cultivator
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
    #26895498 - 08/23/20 02:11 PM (3 years, 5 months ago)

Shit man, that double negative confused the fuck out of me for a minute :lol:  :smokinacandycane:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
OfflineTstone
St.of Circumstance
 User Gallery


Registered: 10/16/18
Posts: 944
Loc: This Planet
Last seen: 25 days, 3 hours
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: tryptkaloids]
    #26895519 - 08/23/20 02:22 PM (3 years, 5 months ago)

Just cause it's white, doesn't mean it's myc. Agar my friend. your playing roulette.


--------------------
Wake now, discover that you are the song that morning brings, but the heart has it's seasons, it's evenings and songs of it's own"


Extras: Filter Print Post Top
OfflineJohn in WI
Neo-Luddite
 User Gallery

Registered: 12/18/13
Posts: 358
Loc: On a hippy trail head ful...
Last seen: 1 day, 16 hours
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush] * 1
    #26895540 - 08/23/20 02:36 PM (3 years, 5 months ago)

I realize the use of peroxide has fallen out of favor--at least on here.  But I don't think it's fair to call it "outdated".  Old data is still good, if it was collected carefully.

I totally agree it isn't the best way to get a good LC.  Cleaning up spores on agar is the best route.  But if the contamination isn't terrible, their seems to be some relevance to using peroxide.  I don't do it on a routine basis, and have never tried it in grain (as some old books suggested).

But in this particular case, it doesn't seem absurd.  I was thinking in my particular case, adding some of the LC/mycellium suspension to a dilute H2O2 solution, allowing it to "work" for a minute, then adding enough (sterilized) sugar solution to bring it up to maybe 2-3%.  It might slow down the myc. briefly, but the sugar solution would quickly consume the left over H2O2, and their would be enough sugar left over to feed the mycellium.

Again, I agree it wouldn't be my first approach--but it isn't a stupid approach either. Acrcane maybe.  Dicey, sure I would agree with that, but it isn't absurd.


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | 5 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Kraken Kratom Red Vein Kratom   North Spore Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* 1st Liquid Culture, arrrgh! Seril 14,347 10 02/28/06 08:35 PM
by Limelight_Liver
* Liquid culture inoculation of straw... possible? The FUnGal FiEnD 7,479 9 05/26/13 04:57 PM
by cronicr
* Easy Liquid Culture Container Tek SixTango 20,279 7 01/17/12 04:59 PM
by dubyastep
* Liquid Cultures to Bulk Substrate Prodijal_Son 17,851 7 05/05/20 03:40 AM
by poisoned
* Starting a simple liquid culture method Garret_Hill 9,137 3 03/11/02 09:49 AM
by YourHighness
* liquid culture failure gray1 5,973 3 07/19/01 09:37 PM
by Azure
* liquid culture using h2o2 baja 9,053 14 03/09/17 10:00 PM
by 3B3B4E
* Liquid culture for alkaloids?? Dr. Slavic 934 3 01/26/03 06:29 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,385 topic views. 27 members, 148 guests and 57 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.011 seconds on 14 queries.