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Fietchen
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
#26899841 - 08/26/20 03:15 AM (3 years, 5 months ago) |
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Cool, the litte Baby Mycelium Growing well... Now i understand why the steps bevore Grain are so important! Learning a lot can't wait to see the Mycelium Growing on Agar.
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maxmush
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
#26900252 - 08/26/20 10:28 AM (3 years, 5 months ago) |
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There you go! Thats what you want to see. You will then test and/or transfer to agar to make sure its clean.
-------------------- Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.
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Fietchen
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
#26904600 - 08/28/20 04:50 PM (3 years, 4 months ago) |
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They are bigger when it's time to jusing it? Can I transfer single baby mycelium to agar ore wait and suck parts from the mycelium in the Syringe?
This is how they looks today
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tryptkaloids
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
#26904628 - 08/28/20 05:08 PM (3 years, 4 months ago) |
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Give it a nice swirl and a couple more days
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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maxmush
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: tryptkaloids]
#26904654 - 08/28/20 05:23 PM (3 years, 4 months ago) |
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Yup wait a few more days and break up the mycelium by giving it a very good agitation by swirling it clockwise and then counter-clockwise (i noticed no magnetic stir bar or marble). You then suck it up with the largest gauge needle you have (16-18g recommended).
So to answer your question: dont use the entire piece of mycelium, break it up first and then use. Use only a very small drop to agar
-------------------- Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.
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A.k.a
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
#26904786 - 08/28/20 06:56 PM (3 years, 4 months ago) |
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I’ve used syringes to suck it up but it’s been a while, I switched to just opening the jar and pouring a little into the grain jar.
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LAGM2020     
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maxmush
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: A.k.a]
#26904869 - 08/28/20 07:57 PM (3 years, 4 months ago) |
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My understanding is he wants to clean it up on Agar first.
-------------------- Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.
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Fietchen
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
#26905486 - 08/29/20 05:53 AM (3 years, 4 months ago) |
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Quote:
maxmush said: My understanding is he wants to clean it up on Agar first.
Agar and maybe a rye grain bag not a big one to grow a few shrooms. My Agar plates arrived the next days
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WoolyMarmot
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: A.k.a]
#26905694 - 08/29/20 09:00 AM (3 years, 4 months ago) |
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Quote:
A.k.a said: I’ve used syringes to suck it up but it’s been a while, I switched to just opening the jar and pouring a little into the grain jar.
hijacking... but, how do you manage this without spillage? I did this and my hand/jar was soaking from all the side spilling
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A.k.a
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: WoolyMarmot]
#26905703 - 08/29/20 09:13 AM (3 years, 4 months ago) |
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Idk, nothing special. I’ve done it with 500ml media bottles and tall half pint jars.
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LAGM2020     
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Fietchen
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: A.k.a]
#26908273 - 08/30/20 04:14 PM (3 years, 4 months ago) |
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Is my LC ready? Looks great can't wait to inject this stuff in my Syringe I will not work with Agar i belive it is to risky to get comtam then LC but you guys maybe disagree but when it's looks good in a syringe why using extra Energie? The risk to get comtam are higher with more steps that i need in Agar. Maybe my Syringe is comtam than agar will to ok i can select clean mycelium but that steps between i don't want much work i do enough with my Plants lol... So LC is my way to go i have made the decision! When i fail my Faul.. Lot of people using this over many years with success too so.
Here is my LC today looks nice but when is ready for my Syringe to inject my rye bag? Thx
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A.k.a
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
#26908282 - 08/30/20 04:17 PM (3 years, 4 months ago) |
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Looks pretty good. You could definitely use it now but you need to get that myc broken up into small pieces.
I like to swirl it fast and then change directions and swirl it the other way suddenly. It kind of makes the myc blobs explode into tiny pieces which is what you want.
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LAGM2020     
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Fietchen
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: A.k.a]
#26908413 - 08/30/20 05:16 PM (3 years, 4 months ago) |
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Quote:
A.k.a said: Looks pretty good. You could definitely use it now but you need to get that myc broken up into small pieces.
I like to swirl it fast and then change directions and swirl it the other way suddenly. It kind of makes the myc blobs explode into tiny pieces which is what you want.
I have bought a magnetic sirere

But i need to put the magnetic in the Glas as sterile as possible than i hope it breaks the Mycelium in small pieces but i bet it will do the job. Cool than i can make my rye Grain socked today and tomorrow i will make my first bag with LC. The rest LC will Grow up again and again ore need to transfer in new LC honey solution?
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Fietchen
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
#26908473 - 08/30/20 05:46 PM (3 years, 4 months ago) |
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It's working
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tryptkaloids
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
#26908512 - 08/30/20 06:09 PM (3 years, 4 months ago) |
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you probably just contaminated your lc by putting the stir bar in it...
You are supposed to pc it in the lc... Or dont use it at all
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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A.k.a
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: tryptkaloids]
#26908541 - 08/30/20 06:22 PM (3 years, 4 months ago) |
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lol yeah better use it quick man before the bacteria multiplies too much.
It looks good though.
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Fietchen
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: tryptkaloids]
#26909038 - 08/31/20 03:32 AM (3 years, 4 months ago) |
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I using itQuote:
tryptkaloids said:
you probably just contaminated your lc by putting the stir bar in it...
You are supposed to pc it in the lc... Or dont use it at all
Rubbing in alcohol was not enough to sterilise? Fuck it
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Fietchen
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
#26909048 - 08/31/20 04:02 AM (3 years, 4 months ago) |
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Wish me luck.. But i have another LC what i not open it again lol
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Fietchen
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
#26909057 - 08/31/20 04:25 AM (3 years, 4 months ago) |
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Shit i suck a litte bit air in the Syringe where the Mycelium is. Is this Bad ore not dramatic? Good i have made another LC so if everything goes wrong i have another opportunity.
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A.k.a
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
#26909099 - 08/31/20 05:19 AM (3 years, 4 months ago) |
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lol 
Sucking air in isn’t good but it might be ok. Did you use the lc on grain yet?
Just remember that lc is very easy to contaminate. Little things that you might get away with for plates and jars will ruin lc. Everything grows much faster in liquid.
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LAGM2020     
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