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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: John in WI]
    #26895544 - 08/23/20 02:38 PM (3 years, 5 months ago)

Hey man try it and let us know how it works :wink:


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Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 10 hours
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: John in WI]
    #26895545 - 08/23/20 02:39 PM (3 years, 5 months ago)

Just sounds like adding fuel to the fire to me. The bottle isnt sterile so you shouldn't put it over your lc...

You said you havent been on the boards for 7 years. In that time nobody even suggests using it anymore. In fact, when i started 5 years ago, several trusted cultivator's suggested it has no use in the hobby


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineFietchen
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Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: John in WI]
    #26895558 - 08/23/20 02:49 PM (3 years, 5 months ago)

Quote:

John in WI said:
I wasn't on this forum for around 8 years, and in that time  I forgot what to search for!  I'm thinking of messing around with it myself. To make an LC with spores directly to solution, then trying to kill bacteria/fungal spores with H2O2 afterwards.  I've never had success using spores or spore solution directly to LC, regardless of how long I pressure cooked the solution.  The problem (for me) is not infected LC solution--it's infected spores. Every spore print or solution are contaminated.  They grow in dirty environments.  Some do have some success going spores to grain, but that is very dicey too.

I'm following this thread and will try and find the peroxide tek.

It might be possible to take your mycellium solution, even if it's contaminated, and put it on agar and clean it up that way? Or perhaps you could go with your mycellium solution to PF cakes, and clean it up like that.  The brown rice flour on vermiculite is supposed to give bacteria a very rough time.  Something to think about.





I hope the Seller i have bought the Syringe in the Netherlands have Clean stuff i think so hopefully.. My LC looks OK to today but i fear that step also. I have lost 1 of my 6 Jars with Rye and i inject Spores direct in the jars I have success with oyster 3 years ago with that technique but maybe with that species it will fail but hope is there.

I will try the Agar method tomorrow but how much cc drops on it at this early stage? Ore waiting a week for more colognising in the LC?


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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26895622 - 08/23/20 03:38 PM (3 years, 5 months ago)

Again OP, your LC isnt ready to do ANYTHING yet. When you see clouds form, thats when you can use it. You only need 1 drop of LC on the agar (.01ml). LESS is better, not more.

If you are getting impatient, this might not be the hobby for you. You can do it tomorrow, but i feel you chances aren't as good as waiting another 5-7 days.

My opinion, forget you got lucky 3 years ago with the oyster. Unlearn that lol. Move forward and use agar properly.


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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OfflineA.k.aM
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
    #26895676 - 08/23/20 04:29 PM (3 years, 5 months ago)

Oyster is a whole different thing too. It’s much more aggressive and contam resistant. Plus I’m pretty sure most places only sell lc to start with.


But yeah starting from spore is going to take longer because it has to germinate first. I’d wait another 4 days minimum and reassess.


--------------------
LAGM2020


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OfflineFietchen
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Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
    #26896324 - 08/24/20 02:13 AM (3 years, 5 months ago)

Quote:

maxmush said:
Again OP, your LC isnt ready to do ANYTHING yet. When you see clouds form, thats when you can use it. You only need 1 drop of LC on the agar (.01ml). LESS is better, not more.

If you are getting impatient, this might not be the hobby for you. You can do it tomorrow, but i feel you chances aren't as good as waiting another 5-7 days.

My opinion, forget you got lucky 3 years ago with the oyster. Unlearn that lol. Move forward and use agar properly.



Quote:

maxmush said:
Again OP, your LC isnt ready to do ANYTHING yet. When you see clouds form, thats when you can use it. You only need 1 drop of LC on the agar (.01ml). LESS is better, not more.

If you are getting impatient, this might not be the hobby for you. You can do it tomorrow, but i feel you chances aren't as good as waiting another 5-7 days.

My opinion, forget you got lucky 3 years ago with the oyster. Unlearn that lol. Move forward and use agar properly.



Quote:

maxmush said:
Again OP, your LC isnt ready to do ANYTHING yet. When you see clouds form, thats when you can use it. You only need 1 drop of LC on the agar (.01ml). LESS is better, not more.

If you are getting impatient, this might not be the hobby for you. You can do it tomorrow, but i feel you chances aren't as good as waiting another 5-7 days.

My opinion, forget you got lucky 3 years ago with the oyster. Unlearn that lol. Move forward and use agar properly.





OK Cool. I have bought this on Ebay later this Day i will buy Agar and everything i need to make this professionell as possible. Cool expiriment with that will learn a lot great!



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OfflineFietchen
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Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26896458 - 08/24/20 06:17 AM (3 years, 5 months ago)

I have bought agar agar than i need malt extract and yiest?


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Offlinesendmehummus
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Registered: 08/03/20
Posts: 383
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26896534 - 08/24/20 08:01 AM (3 years, 5 months ago)

I thought you were just buying a stack of pre made plates off Amazon which is why I only suggested buying those simple accessories. I get pre made plates cause I only need like 25 max a year for my needs.


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Be patient and keep it simple.


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OfflineFietchen
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Registered: 08/27/19
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Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26896684 - 08/24/20 09:35 AM (3 years, 5 months ago)

This the right stuff? Found only this Dry Malt extract call it "backing malt"



Edited by Fietchen (08/24/20 09:35 AM)


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26896879 - 08/24/20 11:04 AM (3 years, 5 months ago)

That should work. I omit the yiest.  Make sure the plates you buy come sterile


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: tryptkaloids]
    #26897266 - 08/24/20 02:30 PM (3 years, 5 months ago)

You can use lots of stuff. I guess dogfood agar used to be common. You could probably just add sugar to the agar also.


--------------------
LAGM2020


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OfflineFietchen
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Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: A.k.a]
    #26897375 - 08/24/20 03:36 PM (3 years, 5 months ago)

Quote:

A.k.a said:
You can use lots of stuff. I guess dogfood agar used to be common. You could probably just add sugar to the agar also.





Than i can bring the malt back for that money and take the cheaper honey :laugh: I thought malt is the?


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OfflineFietchen
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Registered: 08/27/19
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Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: tryptkaloids]
    #26897382 - 08/24/20 03:38 PM (3 years, 5 months ago)

Quote:

tryptkaloids said:
That should work. I omit the yiest.  Make sure the plates you buy come sterile





I hope if not i can drink them in alcohol 70%

Know the problem I don't have a pressure cooker. Can i use a normal boiling pot for the plates?

OK no yiest


Fuck i have bought unsteril plates... I check it to late idiot i am... Better send back ore can i steril them with Aclohol?


Edited by Fietchen (08/24/20 03:42 PM)


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 2 days, 10 hours
Trusted Cultivator
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26897387 - 08/24/20 03:43 PM (3 years, 5 months ago)

It is possible to steam sterilize agar. You'll have to do some digging to find it though. I would reccamend getting a pc asap


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: tryptkaloids]
    #26897550 - 08/24/20 05:33 PM (3 years, 5 months ago)

I think i will buy Glas Plates i can using them over and over again and put them in the oven so why plastic shit? How many Glas plates are recommended for litte Small mushroom cultivation expiriments?


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Invisiblemaxmush
Always learning...

Registered: 06/13/20
Posts: 440
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26897694 - 08/24/20 07:02 PM (3 years, 5 months ago)

You should have at least 20 on hand.


--------------------
Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.


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OfflineFietchen
Stranger
Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: maxmush]
    #26898052 - 08/25/20 02:46 AM (3 years, 5 months ago)

Quote:

maxmush said:
You should have at least 20 on hand.





Than Plastic is recommended? I will buy 20 pack of sterile plates


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OfflineFietchen
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Registered: 08/27/19
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Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26898264 - 08/25/20 07:24 AM (3 years, 5 months ago)

Hey which Size the plates needs? I found steril on ebay but diffrent sizes.


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OfflineA.k.aM
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Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: Fietchen]
    #26898267 - 08/25/20 07:30 AM (3 years, 5 months ago)

Any will work. I think 100mm is normal but not sure.

People use jars or little ketchup cups also.


--------------------
LAGM2020


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OfflineFietchen
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Registered: 08/27/19
Posts: 233
Last seen: 2 years, 8 months
Re: How my Liquid Culture looks like? How long it takes bevore using? [Re: A.k.a]
    #26898377 - 08/25/20 09:15 AM (3 years, 5 months ago)



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