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OfflinephysicalMadness
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Registered: 08/20/20
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Contam or spores germinating overnight?
    #26893141 - 08/22/20 09:15 AM (3 years, 5 months ago)

Hi all, I inoculated this plate by squirting a decently hefty amount from a spore syringe on this plate. Is it possible that they germinated overnight? Or is this a bunch of yeast/bacteria that was in the syringe or something. The plates have been in the fridge for a while and showed no signs of growth so I’d be surprised if that was already on the plate.


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OfflineSpore211215
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Re: Contam or spores germinating overnight? [Re: physicalMadness]
    #26893152 - 08/22/20 09:23 AM (3 years, 5 months ago)

Doesn’t look healthy to me, the plates I’ve ran that looked like that never turned out as myc. Give em another day and see how it goes


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InvisibleGoatrider
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Registered: 04/08/20
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Re: Contam or spores germinating overnight? [Re: Spore211215]
    #26893172 - 08/22/20 09:40 AM (3 years, 5 months ago)

Never store the plates in a fridge.
They`ll push moisture content out and will dry.
A fridge is also a place with lots of bacteria.
You mean you inoculated yesterday?
No chance to see mycelium growing.
It will take up to 3 weeks to see something.


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InvisibleChRnZN
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Registered: 12/21/08
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Re: Contam or spores germinating overnight? [Re: physicalMadness]
    #26893175 - 08/22/20 09:42 AM (3 years, 5 months ago)

:whathesaid:

Looks like contamination to me. It might be worth waiting a week to see if the spores germinate.


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InvisibleRoger Clemency
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Re: Contam or spores germinating overnight? [Re: ChRnZN]
    #26893279 - 08/22/20 10:47 AM (3 years, 5 months ago)

Keeping blank plates in the fridge can also hide contamination. Bacteria might not show itself because of the cold but once you take them out you’ll know.

It’s pretty common to have bacteria in a syringe in my experience so you want to use just a drop of solution and then spread it out with a loop or scalpel tip or just tilt the plate carefully to let the drop run to spread out the spores and hopefully give you a clean spot to transfer.


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