Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Mushroom-Hut Substrate Bags, Substrate Mix

Jump to first unread post Pages: 1
Offlineseand04
It is what it is
 User Gallery


Registered: 12/02/13
Posts: 1,457
Last seen: 15 hours, 18 minutes
Random question πŸ™ƒ
    #26891933 - 08/21/20 02:46 PM (3 years, 5 months ago)

Can you deposit a spore print directly on to an agar plate?


Extras: Filter Print Post Top
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: Random question πŸ™ƒ [Re: seand04]
    #26891937 - 08/21/20 02:49 PM (3 years, 5 months ago)

Thats the idea. Either run a sterile inoculation loop over the print, scrape with a scalpel, or use a sterile swab to get your spores onto agar.


--------------------


Extras: Filter Print Post Top
InvisibleThe Fresh PrintsS
Smell ya later
Male User Gallery


Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air Flag
Trusted Cultivator
Re: Random question πŸ™ƒ [Re: The Fresh Prints]
    #26891942 - 08/21/20 02:51 PM (3 years, 5 months ago)

Or do you mean print directly onto agar? Not sure how you would achieve that without contams.

Also a waste of spores. Print to foil and only use what you need.


--------------------


Edited by The Fresh Prints (08/21/20 02:53 PM)


Extras: Filter Print Post Top
Offlineseand04
It is what it is
 User Gallery


Registered: 12/02/13
Posts: 1,457
Last seen: 15 hours, 18 minutes
Re: Random question πŸ™ƒ [Re: The Fresh Prints]
    #26891943 - 08/21/20 02:51 PM (3 years, 5 months ago)

Quote:

Genghis Chron said:
Thats the idea. Either run a sterile inoculation loop over the print, scrape with a scalpel, or use a sterile swab to get your spores onto agar.



I mean just put a cap on a plate, spores drop and remove the cap. Probably would get contamination tho, just curious πŸ™ƒ


Extras: Filter Print Post Top
Offlineredbee
prole spore threat
Female


Registered: 03/30/20
Posts: 175
Last seen: 1 year, 7 months
Re: Random question πŸ™ƒ [Re: seand04]
    #26891979 - 08/21/20 03:07 PM (3 years, 5 months ago)

Bad ideaβ€”you're covering the plate in spores. Besides that obvious problem, the idea is to scrape absolutely as little as necessary, so you're not picking up contams. The more you deposit, the higher likelihood that you'll get bacteria or mold along with your spores.

Stick to Genghis's advice... unless you wanna run an experiment and show us! :lol:


--------------------
:sporedrop:  redbee's LAGM 2021  :sporedrop:


Extras: Filter Print Post Top
Offlineseand04
It is what it is
 User Gallery


Registered: 12/02/13
Posts: 1,457
Last seen: 15 hours, 18 minutes
Re: Random question πŸ™ƒ [Re: redbee]
    #26891987 - 08/21/20 03:10 PM (3 years, 5 months ago)

Maybe later. Inner stem tissue transfer seems more viable anyway. 🀣🀣🀣


Extras: Filter Print Post Top
OfflineOne of Us
Stranger
 User Gallery


Registered: 03/12/12
Posts: 1,140
Last seen: 6 days, 15 hours
Re: Random question πŸ™ƒ [Re: seand04]
    #26892000 - 08/21/20 03:18 PM (3 years, 5 months ago)

You can print a cap in an unused (no agar/medium) sterile petri if you wanted. That way you can admire it without having to open a foil in the SAB, or compromising its cleanliness in any way. You can also monitor how much is left without opening it.

I thought about doing this, but never did. I like the much smaller form factor of traditional foil prints.


--------------------
LAGM 2021
LAGM 2020
Trade List


Extras: Filter Print Post Top
OfflineMycochopper
Stranger than you
 User Gallery

Registered: 07/15/20
Posts: 82
Last seen: 1 year, 2 days
Re: Random question πŸ™ƒ [Re: seand04]
    #26892009 - 08/21/20 03:20 PM (3 years, 5 months ago)

Put a dab of Vaseline on the lid of the agar plate and the cap onto the Vaseline so it will stay suspended above the agar so only the spores land and the cap never touches.  After printed cap off the dish with a clean lid.


Extras: Filter Print Post Top
OfflineDrGreyThumb
GreyHat


Registered: 08/21/20
Posts: 15
Last seen: 3 years, 4 months
Re: Random question πŸ™ƒ [Re: One of Us]
    #26892018 - 08/21/20 03:25 PM (3 years, 5 months ago)

I've seen people use plates to collect spore drops. They didn't have any agar in the plate though. They just took a proper sized cap, threw it in and closed it for a day or two. Opened it back up and pulled the cap out and just resealed it like he would a regular petri dish. Like I said though, he didn't have agar in his spore print petri dishes.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Mushroom-Hut Substrate Bags, Substrate Mix


Similar ThreadsPosterViewsRepliesLast post
* random questions wind_song 814 2 07/27/01 06:45 PM
by Vitticeps
* Newbie CO2 question Santacloset 737 1 10/24/01 10:41 AM
by puscle
* 2 Questions about Shoebox Monotub (COLONIZATION PROCESS) LumpyNuzz 1,191 12 08/22/20 03:39 AM
by Goatrider
* Terrarium Question Newbie417 1,235 7 05/27/01 12:20 PM
by dump19
* A contam question which I cannot find addressed anywhere.... YSJ 3,892 9 02/08/03 04:45 AM
by BigJohnson
* Vaseline?
( 1 2 all )
Angry Mycologist 2,530 27 12/11/02 04:52 PM
by Angry Mycologist
* applying vaseline to rubber on pc's blood4blood 1,350 11 02/06/08 08:54 PM
by tahoe
* Vaseline for Ultrasonic Humidifing gonnadoit 800 1 06/30/02 03:04 AM
by cheesenoonions

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
95 topic views. 21 members, 160 guests and 47 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.009 seconds on 14 queries.