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seand04
It is what it is



Registered: 12/02/13
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Spore print to agar .
#26891561 - 08/21/20 11:28 AM (3 years, 5 months ago) |
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So I just received a Rustywhyte print and 20 petri dishes. Should I hydrate the spores or just put them straight to the agar. Also I was thinking about making agar mix and then pouring them all then using a flamed exacto knife to put the spores on each. I am waiting for some syringes as well that I want to put on these plates but I think opening the bag of plates would be a mistake before all the spores get here. I really want to do this right. Here is a picture of the petris.
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lookintolearn
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Re: Spore print to agar . [Re: seand04]
#26891564 - 08/21/20 11:32 AM (3 years, 5 months ago) |
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Do you have an inoculation loop? I would use that to put the spores to agar. If you don't they are easily made You usually have better luck spores -> agar than syringe -> agar. Although many people have had success with syringes, me personally they always contam pretty bad.
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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seand04
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Quote:
lookintolearn said: Do you have an inoculation loop? I would use that to put the spores to agar. If you don't they are easily made You usually have better luck spores -> agar than syringe -> agar. Although many people have had success with syringes, me personally they always contam pretty bad.
I don't have a loop🥺. How can I make one
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seand04
It is what it is



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Re: Spore print to agar . [Re: seand04]
#26891572 - 08/21/20 11:35 AM (3 years, 5 months ago) |
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I am going to use agar, food coloring, honey and potato flakes for the agar mix.
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Gan
Wielder of Narya



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Re: Spore print to agar . [Re: seand04]
#26891579 - 08/21/20 11:39 AM (3 years, 5 months ago) |
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Can make a loop like this: https://www.shroomery.org/forums/showflat.php/Number/24292526#24292526
Or you can use a sterilized scalpel blade as a last resort
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seand04
It is what it is



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Re: Spore print to agar . [Re: seand04]
#26891581 - 08/21/20 11:41 AM (3 years, 5 months ago) |
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Quote:
seand04 said:
Quote:
lookintolearn said: Do you have an inoculation loop? I would use that to put the spores to agar. If you don't they are easily made You usually have better luck spores -> agar than syringe -> agar. Although many people have had success with syringes, me personally they always contam pretty bad.
I don't have a loop🥺. How can I make one
Looked it up. Bodhisattva has a great one. Thanks for everything.
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seand04
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Re: Spore print to agar . [Re: Gan]
#26891587 - 08/21/20 11:43 AM (3 years, 5 months ago) |
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Quote:
Gan said: Can make a loop like this: https://www.shroomery.org/forums/showflat.php/Number/24292526#24292526
Or you can use a sterilized scalpel blade as a last resort
Thanks I just was reading this. Great stuff, I am going to make one now. Will copper wire work? I have a lot of thin copper wire.
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lookintolearn
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Re: Spore print to agar . [Re: seand04]
#26891706 - 08/21/20 12:51 PM (3 years, 5 months ago) |
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I think copper should work but don't quote me. Personally I bought one off amazon for like 10$ came with two loop holders and like 50 extra disposable pre-made loops. Great investment! And also you should look into MEA. (Light malt extract agar) I tried potato agar for my first go and the viability is mediocre at best. MEA always comes out super clear for me and since I use pastyplates I need all the clear I can get haha.
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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seand04
It is what it is



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Quote:
lookintolearn said: I think copper should work but don't quote me. Personally I bought one off amazon for like 10$ came with two loop holders and like 50 extra disposable pre-made loops. Great investment! And also you should look into MEA. (Light malt extract agar) I tried potato agar for my first go and the viability is mediocre at best. MEA always comes out super clear for me and since I use pastyplates I need all the clear I can get haha.
Awesome, I am going to look it up right now . The plates will stay sterile in the package until then. I wanna get everything ready so I can do it right💪💪💪
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dirtybirdx
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Re: Spore print to agar . [Re: seand04]
#26891769 - 08/21/20 01:26 PM (3 years, 5 months ago) |
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If you’re relatively new, you shouldn’t try making a syringe with your spores first.
Totally do it, for practice, but to give yourself the best chance at what you’re trying to accomplish, it’s simpler to put the spores directly to agar.
You could buy a pack of cheap sterile swabs at the pharmacy and use one to scrape a bit of spores onto your agar. I’m sure you could even just pour some out onto your agar although risk for contamination may be slightly higher with that method - the reasoning being you’re hovering your hand and a tin foil packet over your open media and shaking - contains canfall off and settle in the plate.
Using a tiny swab with concise deliberate movements leaves less of a window for that.
Just do a search with ‘spore to agar’ and you can find people giving step by steps with pics.
👍
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trippytimes
♡contamination queen♡



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Re: Spore print to agar . [Re: seand04]
#26891790 - 08/21/20 01:36 PM (3 years, 5 months ago) |
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Quote:
seand04 said:
Quote:
lookintolearn said: Do you have an inoculation loop? I would use that to put the spores to agar. If you don't they are easily made You usually have better luck spores -> agar than syringe -> agar. Although many people have had success with syringes, me personally they always contam pretty bad.
I don't have a loop🥺. How can I make one
You could use a copper wire. me personally I actually just use a spore syringe that's empty and heat up the needle to scrape the print. Or scalpel but I prefer the needle because it's easier to burn and sanitize. Plus It has a nice handle for grip.just make sure you wipe it down before you put it into your sab. Inoculation loops can be made from anything basic wire I have made them out of paper clips also for warning when it comes to spores a little goes a long way. I personally do not make syringes to agar. Now if I get my hands on a spore syringe I will put it in agar but it is hard to just barely get a drop on there. Hope this helps.
Edited by trippytimes (08/21/20 01:39 PM)
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maxmush
Always learning...

Registered: 06/13/20
Posts: 440
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I find i have better control with a sterilized swab. I just PC them in a mason jar.
If you want a quick and easy inoculation loop, just use a twist tie attached to a pen. Strip the plastic coating off and twist making a loop and the end. Its that easy.
-------------------- Disclaimer: all information presented is intended for educational purposes only. All photos are only representations and not directly from the user.
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lookintolearn
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Also you do have a pressure cooker correct? I use that to sterilize everything from tools to jars (of course), But one of my favorite uses is making pastyplates. They are glad minirounds modified to use agar in. You can pick those up at the store and play around with them till you are ready to do your pours in petris I personally haven't even moved on to petris because pastyplates are fool-proof and I haven't found a need to. Eventually, I will once I have 50 transfers I need to make at once haha.
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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dirtybirdx
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Quote:
lookintolearn said: Also you do have a pressure cooker correct? I use that to sterilize everything from tools to jars (of course), But one of my favorite uses is making pastyplates. They are glad minirounds modified to use agar in. You can pick those up at the store and play around with them till you are ready to do your pours in petris I personally haven't even moved on to petris because pastyplates are fool-proof and I haven't found a need to. Eventually, I will once I have 50 transfers I need to make at once haha.
No pours are cool, but you may finally decide to switch to petris once you want to start showing off 
-------------------- Need help?

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seand04
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Re: Spore print to agar . [Re: dirtybirdx]
#26891877 - 08/21/20 02:20 PM (3 years, 5 months ago) |
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I make pasty plates. I have 10 growing right now. I am gonna try the petris. I am doing spore prints and tissue transfer.
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lookintolearn
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Re: Spore print to agar . [Re: seand04]
#26891908 - 08/21/20 02:34 PM (3 years, 5 months ago) |
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Ahh very nice yeah I do want to show off eventually ! I have some pretty transfers but they aren't quite picture perfect yet. And seand make sure you get one of those 500ml media bottles for pouring. Bod recommended that and it's fucking awesome. I just make up like 1000ml between two bottles chuck it in the PC and it's good for a long time I just happen to use it for no pours because I don't feel like making up agar everytime I want to make plates.
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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seand04
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Awesome brother, I am going to check out the bottles. I was just thinking of using a clean grain jar. Is there a link for the bottles?
Edited by seand04 (08/21/20 02:43 PM)
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dirtybirdx
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When I first did agar 4 years ago I started with pasty plates too. Handy for sure. I may incorporate no pours again. I still can’t walk down that aisle in the store and not look at ziploc twist-locs and glad mini-rounds 🤣🤣. So weird.
I will say that d3’s perfect agar transfer tek (using the punch) is an instant level up. If you go petris, you won’t regret doing it his way, especially if you’re in it to get the eye-candy like me 😳
-------------------- Need help?

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dirtybirdx
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Re: Spore print to agar . [Re: dirtybirdx]
#26891951 - 08/21/20 02:53 PM (3 years, 5 months ago) |
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-------------------- Need help?

Edited by dirtybirdx (08/21/20 02:55 PM)
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seand04
It is what it is



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Re: Spore print to agar . [Re: dirtybirdx]
#26891964 - 08/21/20 02:59 PM (3 years, 5 months ago) |
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I just got this stuff. I will post updates
Edited by seand04 (08/21/20 03:29 PM)
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