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Offlinegman144
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Registered: 08/19/20
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Poly-fil is growing in my agar!
    #26890240 - 08/20/20 03:00 PM (3 years, 5 months ago)

I swear it looks just like poly-fil, but it ain't and its growing.  I have this growing in six agar plates and need to know what the F it is so I can decontaminate it and my procedures.  Surely there's an answer and surely this is the place to get it, I hope!

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Offlinemistermycelium
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Re: Poly-fil is growing in my agar! [Re: gman144]
    #26890286 - 08/20/20 03:51 PM (3 years, 5 months ago)

Cobweb mold. Esp if its growing really really fast.


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OfflineGastronomicus
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Re: Poly-fil is growing in my agar! [Re: mistermycelium]
    #26890349 - 08/20/20 04:31 PM (3 years, 5 months ago)

:whathesaid:

You can maybe snag a transfer off of the top right corner of the first pic but the others look screwed. Sorry


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Make my Funk the P Funk, I wants to get Funked up

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Offlinegman144
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Re: Poly-fil is growing in my agar! [Re: Gastronomicus]
    #26890437 - 08/20/20 05:20 PM (3 years, 5 months ago)

I hear ya, yet it was my understanding that cobweb mold was a casing/mushroom thing, not an agar thing.  I also have it on agar I poured with nothing else in it.  Just appeared.

The reason I need to know is because I am clearly doing something wrong or something is in the air and I'm not managing it very well.  Almost all my agar dishes have it to one degree or another.


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InvisibleRoger Clemency
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Re: Poly-fil is growing in my agar! [Re: gman144]
    #26890486 - 08/20/20 05:54 PM (3 years, 5 months ago)

It’s probably getting in while you’re pouring your plates if it’s showing up on blank plates. If it wasn’t sterilized enough I think there would be bacteria.

It looks cobwebby but that’s just one of the things floating around your area. Do you use a SAB?


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OfflineGastronomicus
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Re: Poly-fil is growing in my agar! [Re: gman144]
    #26890695 - 08/20/20 08:26 PM (3 years, 5 months ago)

I've been getting the same thing whenever I try to take clones. Pretty sure it's my SAB technique. Practice and make more plates. It'll get cleaned up eventually


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Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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Offlinegman144
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Re: Poly-fil is growing in my agar! [Re: Roger Clemency]
    #26890788 - 08/20/20 09:20 PM (3 years, 5 months ago)

Wow, been trying to review and replay how I may have screwed up the agar and never considered that the plates could have been compromised. That would explain everything! Time for another transfer sesh. Thanks!!


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