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OfflinephysicalMadness
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Registered: 08/20/20
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Interesting Contam Identification Question
    #26890134 - 08/20/20 01:48 PM (3 years, 5 months ago)

This is my first post. I squirted some of a spore syringe of GT on this plate about a week ago. There is no mycelium besides this. I am also wondering why spores haven’t germinated at all.
I am just wondering what this is. It is interesting because it’s kinda blue or green but it surrounded by white mycelium. Both of which are expanding.
Also, should I parafilm plates right when I inoculate or later?
Many thanks.





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OfflineJatzan
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Registered: 07/21/20
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Re: Interesting Contam Identification Question [Re: physicalMadness]
    #26890163 - 08/20/20 02:11 PM (3 years, 5 months ago)

Contams happen, you can remove them with transfers. The contam is not from the syringe, because its on the side of the plate.

What puzles me is how you got no mycelium at all. Did you shake the syringe well before use? What is your agar recipe, <2% agar <2% nutes (malt,potatoe etc)?

Also welcome to shroomery :laugh:


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OfflinephysicalMadness
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Re: Interesting Contam Identification Question [Re: Jatzan]
    #26890175 - 08/20/20 02:21 PM (3 years, 5 months ago)

Thanks for the response. I thought I shook it well. I see a tiny bit on my grain but not much. It makes me wonder if the syringe was garb.  Could have a very low infectious unit concentration.  Am I allowed to post the supplier?
I still just want to know what mold is greenish with white around the edge.
My recipe is janky as I scraped the materials together. But it’s 2% agar, 2% yeast extract, 2% purified dextrose. I have pink and king oyster growing well on them.


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Invisiblenatedawgnow
Rocky mountain hood rat
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Registered: 02/09/15
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Re: Interesting Contam Identification Question [Re: physicalMadness]
    #26890193 - 08/20/20 02:32 PM (3 years, 5 months ago)

Most contam molds are white with green spores. That one looks like aspergillus
but I am having a hard time making out definite features.

Also, there is no ruling out that the contam came from the syringe if you
just squirted it onto the plate. If you put a drop on a loop and swiped and the
contam was outside the swipe zone, then maybe you could rule it out but a
drop can travel across a plate and put contams anywhere including near the edge.


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OfflineJatzan
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Re: Interesting Contam Identification Question [Re: natedawgnow]
    #26890202 - 08/20/20 02:35 PM (3 years, 5 months ago)

Yeah, thats true. I assumed he put just a wee bit in the middle of the plate.

Also dont post the supplier.


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