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Offlinedebord
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G2G contaminated jars
    #26889471 - 08/20/20 05:38 AM (3 years, 5 months ago)

Been having lots of problems with contamination - both in tubs spawing with what seemed like healthy jars and now I just did 10 G2G from what I thought was a good jar and they all started going well and then went blue / green like these pictures.



Can anyone give me some advice please?
Start again with new spores / LC?


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OfflineMycochopper
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Re: G2G contaminated jars [Re: debord]
    #26889483 - 08/20/20 05:56 AM (3 years, 5 months ago)

Are you using proper sterile techniques via Flow hood and or SAB or just transferring in open air?


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Offlinedebord
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Re: G2G contaminated jars [Re: Mycochopper]
    #26889496 - 08/20/20 06:03 AM (3 years, 5 months ago)

Quote:

Mycochopper said:
Are you using proper sterile techniques via Flow hood and or SAB or just transferring in open air?




I am using a SAB and lots of oust spray and lots of alcohol to clean.


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Offlinelateforthafuture
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Re: G2G contaminated jars [Re: debord]
    #26889501 - 08/20/20 06:08 AM (3 years, 5 months ago)

How are you inoculating the master jar? If you are using a LC for the master jar...how are you inoculating the LC?


--------------------
"Not what could have been, or what should have been, but what will be" -Self
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OfflineMycochopper
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Re: G2G contaminated jars [Re: lateforthafuture]
    #26889519 - 08/20/20 06:17 AM (3 years, 5 months ago)

Your grain looks a little wet to me.  How are you doing your grain process?


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Offlinedebord
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Re: G2G contaminated jars [Re: lateforthafuture]
    #26889528 - 08/20/20 06:25 AM (3 years, 5 months ago)

Quote:

lateforthafuture said:
How are you inoculating the master jar? If you are using a LC for the master jar...how are you inoculating the LC?




Commercially bought spore syringes for the master jar.


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Offlinedebord
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Re: G2G contaminated jars [Re: Mycochopper]
    #26889529 - 08/20/20 06:27 AM (3 years, 5 months ago)

Quote:

Mycochopper said:
Your grain looks a little wet to me.  How are you doing your grain process?




Grain is soaked overnight, boiled for abut 10 minutes to get the heat, then colander and then put on trays to dry, then 90 mins in the PC.


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OfflineA.k.aM
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Re: G2G contaminated jars [Re: debord]
    #26889534 - 08/20/20 06:29 AM (3 years, 5 months ago)

It’s almost definitely the syringe.

Some contams move much quicker than myc, but seem to take a while to get started so even though the syringe is dirty the myc gets established first.

But then if you g2g it the contam has already woken up but is being held back by the myc, and once you dump it to a fresh jar the mold will spread faster than the mycelium.


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Edited by A.k.a (08/20/20 06:30 AM)


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Offlinedebord
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Re: G2G contaminated jars [Re: A.k.a]
    #26889550 - 08/20/20 06:43 AM (3 years, 5 months ago)

Thanks.
I will start again.
I've seen some LC online so I might go for that - it's quicker for spawning I believe?


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OfflineMycochopper
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Re: G2G contaminated jars [Re: debord]
    #26889565 - 08/20/20 06:51 AM (3 years, 5 months ago)

Quote:

debord said:
Thanks.
I will start again.
I've seen some LC online so I might go for that - it's quicker for spawning I believe?




LC online hopefully will be good but since you didn't do it yourself may also be contaminated.  Perhaps spore to agar and then transfer to get contams out and then to LC may be better for clean myc.  May take longer but worth the wait.


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Offlinedebord
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Re: G2G contaminated jars [Re: Mycochopper]
    #26889610 - 08/20/20 07:24 AM (3 years, 5 months ago)

Looks like it's time to learn Agar then!


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OfflineJoBloShow
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Re: G2G contaminated jars [Re: debord]
    #26889631 - 08/20/20 07:46 AM (3 years, 5 months ago)

Agar, agar, agar.
Like a lot of people, I used to be intimidated by it.
Now, I can't believe I went so long without it.
:mushdance:


--------------------
Following our will and wind we may just go where no one's been,
We'll ride the spiral to the end and may just go where no one's been...



FIND THE BEAUTY IN THE DISSONANCE


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OfflineMycochopper
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Re: G2G contaminated jars [Re: JoBloShow]
    #26889709 - 08/20/20 08:46 AM (3 years, 5 months ago)

So much you can do with agar and its really easy.  I truly enjoy the entire process.


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