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OfflineLoppy1512
Registered: 07/20/20
Posts: 186
Loc: Ommmmmm
Last seen: 6 days, 2 hours
Help identifying contams and myc on my agar plates
    #26884718 - 08/17/20 11:43 AM (3 years, 5 months ago)

Hi all,

I've recently inoculated my first agar plates. I used LC syringes as they were the only things i could get hold of. On a couple of the plates I squirted a few drops and it made a mess and on the others I squirted the LC into a sterile shot glass and then used a loop to smear my plates.

On the Aug 12 I nocced up 5 plates each of PE6, PESA, GT and Natal Super Strength. In case I had buggered things up, I nocced up another 5 plates of each on Aug 14.

I've started to see growth already but since it's my first time I'm not sure what I'm looking at. Could anyone help me out with regards to how things are looking?



Edited by Loppy1512 (08/17/20 11:44 AM)


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OfflineCanebrake
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Registered: 05/01/20
Posts: 96
Last seen: 6 months, 14 minutes
Re: Help identifying contams and myc on my agar plates [Re: Loppy1512]
    #26884775 - 08/17/20 12:15 PM (3 years, 5 months ago)

Too me it all looks horrible. Let it grow more.


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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 2,872
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Re: Help identifying contams and myc on my agar plates [Re: Canebrake]
    #26884804 - 08/17/20 12:36 PM (3 years, 5 months ago)

Take a wedge from the first picture, fast.


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OfflineTrust_d Cultivator
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Registered: 08/15/20
Posts: 92
Last seen: 10 months, 14 days
Re: Help identifying contams and myc on my agar plates [Re: smalltalk_canceled]
    #26885090 - 08/17/20 03:03 PM (3 years, 5 months ago)

The first GT plate is workable.


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OfflineLoppy1512
Registered: 07/20/20
Posts: 186
Loc: Ommmmmm
Last seen: 6 days, 2 hours
Re: Help identifying contams and myc on my agar plates [Re: Trust_d Cultivator]
    #26886917 - 08/18/20 02:53 PM (3 years, 5 months ago)

Thanks for the comments, so you reckon apart from that GT plate the rest is are contams and should be chucked or let them grow out more to see?


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Offlinedirtybirdx
getting better
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Registered: 07/28/20
Posts: 250
Last seen: 2 years, 4 months
Re: Help identifying contams and myc on my agar plates [Re: Loppy1512]
    #26887213 - 08/18/20 05:53 PM (3 years, 5 months ago)

Quote:

Loppy1512 said:
Thanks for the comments, so you reckon apart from that GT plate the rest is are contams and should be chucked or let them grow out more to see?





If it were me, I would probably work with these two plates.

In the pic with the red and purple, I would first look to take the red piece, but knowing that your other plates got contaminated in the agar trenches from your scalpel or loop, I might be weary taking a piece over there and may look at the purple. Or both.



--------------------
Need help?




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OfflineLoppy1512
Registered: 07/20/20
Posts: 186
Loc: Ommmmmm
Last seen: 6 days, 2 hours
Re: Help identifying contams and myc on my agar plates [Re: dirtybirdx]
    #26889460 - 08/20/20 05:23 AM (3 years, 5 months ago)

thanks everyone, I've done the transfers as suggested...


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