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Offlineboomer_beans
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First time grow - using PF Tek - seeking advice on potential contamination
    #26889251 - 08/19/20 10:14 PM (3 years, 5 months ago)

Greetings :mushroom2: -

As the subject line reads, this is my first attempt at cultivating mushrooms - I am following the PF Tek guidelines, and have run into some potential issues with two of my jars.

It has been a week since inoculation, and two of the jars have yellowish growth to them (presumably myc piss?).  I am asking 2 things:

1. Are these jars contaminated to the point of no return, or may I salvage them yet?

2. What can I do next time to prevent this yellowing (I followed the instructions essentially verbatim)?

I had an epic trip in Lake Tahoe this past weekend - it was pouring out and sunny at the same time - it was perfect.


http:// Appreciate the insight to come, and happy tripping!


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InvisibleRhizomorph
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26889266 - 08/19/20 10:48 PM (3 years, 5 months ago)

Those look beyond salvageable to me. The third picture looks fine if that jar is independent from the other two pictures. I'd toss them.

There's a good chance that your syringe was contaminated depending on where you got it. (of course no syringe is truly 100% contaminant-free) I highly recommend using syringes/prints from the sponsors on the shroomery and putting them to agar (A pressure cooker is highly recommended but not necessary to work with agar).

Additionally you can try steam sterilizing for longer if you're using a pot instead of a pressure cooker. A tight lid is essential for this but I found my lid wasn't quite tight enough and leaving it on the stove for an extra 30 minutes seemed to do the trick.

All of this is assuming you followed the pf tek to a T as you say you did.


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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Offlineboomer_beans
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: Rhizomorph]
    #26889307 - 08/20/20 12:08 AM (3 years, 5 months ago)

Thank you for the response - I left the jars covered on the stove for about an 1 hr 40 mins to be safe, but they do seem to be contaminated.

I'm going to take your advice and get rid of them - I will update with photos when I move on to the next steps with the jar that is not contaminated.

Stay tuned!


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InvisibleRhizomorph
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26889350 - 08/20/20 01:26 AM (3 years, 5 months ago)

That's probably your best bet :thumbup:

If you get a decent sized mushroom off that cake I recommend cloning it to agar to get good genetics for future grows.

Agar is the way to go though for real. You could take a culture that is mixed with 5 different kinds of bacteria & mold, and with some precision, you'd be able to clean the culture up and ensure it has no contamination after a transfer or two.

I hope your last cake works out. Third time is a charm - crossing my fingers for ya! :goodluck:


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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Offlineboomer_beans
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: Rhizomorph]
    #26890409 - 08/20/20 05:06 PM (3 years, 5 months ago)

Appreciate it - will update soon


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Offlinenaturespeaks2me
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26890859 - 08/20/20 10:37 PM (3 years, 5 months ago)

toss first 2 keep 3rd and look at lots of info on being sterile . gl. mushluv


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Offlineboomer_beans
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26923974 - 09/07/20 11:27 PM (3 years, 4 months ago)

Update:

Below are updated images of the 2 PF-tek jars that seem to be doing their thing. 

Jar 1 - (almost fully colonized, maybe 4-5 more days, pulling away from the sides of the jar, visible condensation)




Jar 2 - (doing well, but the back side has to connect still)







Stay tuned ... :mushroom2:


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InvisibleRhizomorph
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26924319 - 09/08/20 08:55 AM (3 years, 4 months ago)

Looking good!
:thumbsup:


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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Offlineboomer_beans
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: Rhizomorph]
    #26969967 - 10/04/20 11:43 PM (3 years, 3 months ago)




This is the first flush so far from the jars posted above - would love any feedback regarding how these look and when I should pick them

Cheers

:mushroom2:


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InvisibleRhizomorph
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26969987 - 10/05/20 12:08 AM (3 years, 3 months ago)

Pick them around the time the veil breaks for best potency. if you want to take a spore print or you just want pretty looking mushrooms you can wait till the cap flattens out though. It won't reduce the potency significantly if you do this.

I usually just harvest whenever is convenient once the veil breaks :shrug:


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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Offlinekarri0n
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: Rhizomorph]
    #26969990 - 10/05/20 12:13 AM (3 years, 3 months ago)

I think those are pint jars.

And I think they are the same knockoff brand pint jars(or lids anyway) that I have.

I believe this is why you have had not so stellar success - the pint jars are prone to contamination and I believe this is due to less GE than the 1/2 pint jars. I am having similar issues.

I was completely unable to find 1/2 pint jars myself. COVID has made finding mycology supplies tough.


Edited by karri0n (10/05/20 12:14 AM)


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InvisibleRhizomorph
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: karri0n]
    #26970023 - 10/05/20 12:59 AM (3 years, 3 months ago)

Quote:

karri0n said:the pint jars are prone to contamination and I believe this is due to less GE than the 1/2 pint jars. I am having similar issues.



Gas exchange is mediated by the modifications you make to the lid so the original lid design of the jars wouldn't impact gas exchange at all unless they were damaged from the get-go.

Half pint jars will be less contaminant-prone since they will colonize faster (giving contaminants less time/chance to take hold). However, if your sterile techniques are down you shouldn't have an issue either way. Many people inoculate quart sized jars which are 4x the size of a half pint.

OP's jar looks like a half pint to me but I could be wrong.


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


Edited by Rhizomorph (10/05/20 01:21 AM)


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Offlineboomer_beans
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: Rhizomorph]
    #26970238 - 10/05/20 08:02 AM (3 years, 3 months ago)

Appreciate the feedback - my main question now is regarding the mushrooms that have grown and whether or not they seem healthy in your experience (there is white fuzz growing at the base of every stem, but I’ve read that’s probably due to lack of FAE and could just be mycelium) - has anyone else run into this?


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InvisibleRhizomorph
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26970329 - 10/05/20 09:48 AM (3 years, 3 months ago)

They look spot on :rockon:

They will always have a bit of white fuzz growing at the stem. It is only when there is a lot of very thick fuzz or the fuzz looks like it is "reaching" out off the base of the mushroom that they need more FAE.

Search up pictures of "fuzzy feet" and you will see the difference. Your mushrooms are perfectly healthy!


--------------------

:cookiemonster: Major Issues in the Psychedelic Movement: Why 'Psychedelic People' and the Psychedelic Movement Sucks:elmo:

:awesomenod: Easiest No-Pour Agar Method: Alien's Holy Grail No pour Agar unmodified containers:awesomenod:


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Offlinekarri0n
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26970331 - 10/05/20 09:49 AM (3 years, 3 months ago)

Quote:

boomer_beans said:
Appreciate the feedback - my main question now is regarding the mushrooms that have grown and whether or not they seem healthy in your experience (there is white fuzz growing at the base of every stem, but I’ve read that’s probably due to lack of FAE and could just be mycelium) - has anyone else run into this?




Yeah that's what I have read as well. I'd say for your first attempt, you got fruits, that's a definite success story mate!

Fuzzy feet won't hurt anything, just means you aren't yet at the proficiency of a TC or someone with a professional grow room and a Martha and perfect conditions.


--------------------

Panaeolus Bisporus


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Offlinekarri0n
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26970338 - 10/05/20 09:55 AM (3 years, 3 months ago)

Quote:

boomer_beans said:
Appreciate the feedback - my main question now is regarding the mushrooms that have grown and whether or not they seem healthy in your experience (there is white fuzz growing at the base of every stem, but I’ve read that’s probably due to lack of FAE and could just be mycelium) - has anyone else run into this?




I meant from the shape, not the lid. TC's tend to say short and flat is better for pf cakes, and that it's because the air helps the myc grow while lack thereof is going to help anaerobic bacteria grow. Cron references this with his squat containers too.

I ended up needing to loosen the lids on my pf jars because my holes were too small for the needle and were thereby not providing enough GE for the tall jars. I used a thin drill bit instead of a nail. Didn't follow the tek and the simplest thing bit me in the ass.

Edit: you're seeing Quart size PF cakes? I've never seen that.


--------------------

Panaeolus Bisporus


Edited by karri0n (10/05/20 09:58 AM)


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Offlineboomer_beans
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: karri0n]
    #26971022 - 10/05/20 06:01 PM (3 years, 3 months ago)

Awesome - thanks for the feedback. I went ahead and pulled most of these off to start drying and am re-dunking the cakes for round 2


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OfflineWarezMyDinrBitc
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Re: First time grow - using PF Tek - seeking advice on potential contamination [Re: boomer_beans]
    #26971027 - 10/05/20 06:03 PM (3 years, 3 months ago)

Yeah if they have fuzzy bases you don't have enough fresh air exchange.


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