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OfflineDr. Greenthumb
HipHopHead 4Life

Registered: 12/15/99
Posts: 333
Last seen: 3 months, 8 days
What exactly do YOU do when you innoculate?
    #2688876 - 05/16/04 11:15 PM (12 years, 11 months ago)

List your steps in detail.  Anything from taking a shower to wipeing everything down with Lysol to doing a little dance or whatever you do.  Maybe I can pick up some tips. :laugh:


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InvisibleMykey
spectraltraveler

Registered: 04/07/04
Posts: 542
Re: What exactly do YOU do when you innoculate? [Re: Dr. Greenthumb]
    #2688937 - 05/16/04 11:27 PM (12 years, 11 months ago)

Shower. Lysol wipe the any work surfaces. Turn off any fans a while before. Use anti bacterial soap and preferably gloves. Spray oust into the air liberally to kill any airbourne bacteria and mold. Flame and alcohol wipe the needle between each jar. Wear a dust mask.

That is about it.
The oven tek is a good way to help reduce contam rates.

The very best IMO is a good glovebox. You can go a step further and make a positive pressure glovebox with a rubbermaid and a cheap little hepa/ion unit from walmart. Total cost is about 45 bucks ,but it is well worth it.

Good Luck!
MYKEY


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InvisibleATWAR
Connoisseur

Registered: 01/26/03
Posts: 1,640
Loc: #108768 in line...
Re: What exactly do YOU do when you innoculate? [Re: Mykey]
    #2689191 - 05/17/04 12:25 AM (12 years, 11 months ago)

Quote:

Mykey said:
The oven tek is a good way to help reduce contam rates.





Not sure if you have seen this, but it is worth a look...

Oven tek test...


--------------------
To give is to live...



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Anonymous

Re: What exactly do YOU do when you innoculate? [Re: Dr. Greenthumb]
    #2689684 - 05/17/04 02:13 AM (12 years, 11 months ago)

-spray lysol
-pray for contams to be eliminated/guided away(while lysol settles)
-suck sterile air into syringe over flame
-shake syringe
-push air out
-knock up jar
-wipe needle with alcohol/flame
-knock up jar....etc


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InvisibleTRS
The SneakyMastermind
Registered: 10/13/03
Posts: 374
Re: What exactly do YOU do when you innoculate? [Re: ]
    #2689713 - 05/17/04 02:25 AM (12 years, 11 months ago)

i dont go into too much trouble to keep everything clean so heres what i do. by the way, i buy packs of ten insulin syringes because i cant find anything else and they work fine for me.

wash hands. get a glass and put some boiled water in it and microwave it for about a minute. while thats cooling i get my jars and syringes ready. when the water has cooled i wash my hands again and scrape off some spores (from a print) with a knife that i wiped with disinfectant. the spores go into the water and i mix it a bit. i grab a jar, take off the foil and innoculate. ill usually use a syringe for a few jars, then ill grab another syringe. i do that till all jars are knocked up then i put them away for a few weeks. all of this is done in open air on the kitchen counter.


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Offlineel_dusto
consciousness-spelunker
Registered: 05/19/04
Posts: 6
Last seen: 12 years, 10 months
Re: What exactly do YOU do when you innoculate? [Re: Dr. Greenthumb]
    #2701894 - 05/19/04 04:47 PM (12 years, 11 months ago)

step 1: wash hands w/AB soap

step 2: remove tinfoil

step 3: inoculate

step 4: watch them all colonize successfully

(^_^)


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Amazon Shop for: ½ Pint Jars, HEPA Filter

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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